Efficient mechanism of DNA repair stabilizes genome of Arabidopsis thaliana from the Chernobyl zone

Efficient mechanism of DNA repair stabilizes genome of Arabidopsis thaliana from the Chernobyl zone

Tolerance to radiomimetics and heavy metals has been investigated in Arabidopsis thaliana plants collected in the Chernobyl zone. Decrease of root growth and DNA damage level of a single cell have been evaluated. Tolerance of A. thaliana from the zone to the growth on genotoxic medium has been rev...

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Дата:2017
Автори: Shevchenko, G.V., Talalaiev, O.S.
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Опубліковано: Видавничий дім "Академперіодика" НАН України 2017
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Цитувати:Efficient mechanism of DNA repair stabilizes genome of Arabidopsis thaliana from the Chernobyl zone / G.V. Shevchenko, O.S. Talalaiev // Доповіді Національної академії наук України. — 2017. — № 4. — С. 84-90. — Бібліогр.: 14 назв. — англ.

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spelling irk-123456789-1265642017-11-27T03:02:54Z Efficient mechanism of DNA repair stabilizes genome of Arabidopsis thaliana from the Chernobyl zone Shevchenko, G.V. Talalaiev, O.S. Біологія Tolerance to radiomimetics and heavy metals has been investigated in Arabidopsis thaliana plants collected in the Chernobyl zone. Decrease of root growth and DNA damage level of a single cell have been evaluated. Tolerance of A. thaliana from the zone to the growth on genotoxic medium has been revealed. It is noted that certain Arabidopsis plants recover genomic DNA faster than control ones collected outside of the zone. Screening revealed plant lines expressing various levels of tolerance to genotoxins. Досліджено стійкість проростків Arabidopsis thaliana із зони Чорнобильської АЕС до дії мутагенів (радіоміметиків і важких металів). Проаналізовано швидкість росту коренів і ступінь пошкоджень ДНК окремих клітин рослин. Виявлено стійкість росту A. thaliana із зони на генотоксичному середовищі і встановлено, що деякі рослини із зони ЧАЕС відновлюють свій геном швидше, ніж контрольні, зібрані поза зоною. За результатами скринінгу визначено рослини різного ступеня чутливості до дії генотоксинів. Исследована устойчивость проростков Arabidopsis thaliana из зоны Чернобыльской АЭС к действию мутагенов (радиомиметиков и тяжелых металлов). Проанализированы скорость роста корней и степень повреждения ДНК единичной клетки растений. Обнаружена устойчивость роста A. thaliana из зоны на генотоксичных средах и установлено, что некоторые растения из зоны ЧАЭС восстанавливают свой геном быстрее, чем контрольные, собранные вне зоны. В результате скрининга определены растения различной степени толерантности к воздействию генотоксинов. 2017 Article Efficient mechanism of DNA repair stabilizes genome of Arabidopsis thaliana from the Chernobyl zone / G.V. Shevchenko, O.S. Talalaiev // Доповіді Національної академії наук України. — 2017. — № 4. — С. 84-90. — Бібліогр.: 14 назв. — англ. 1025-6415 DOI: doi.org/10.15407/dopovidi2017.04.084 http://dspace.nbuv.gov.ua/handle/123456789/126564 577.215:582.683.2:621.311.25 en Доповіді НАН України Видавничий дім "Академперіодика" НАН України
institution Digital Library of Periodicals of National Academy of Sciences of Ukraine
collection DSpace DC
language English
topic Біологія
Біологія
spellingShingle Біологія
Біологія
Shevchenko, G.V.
Talalaiev, O.S.
Efficient mechanism of DNA repair stabilizes genome of Arabidopsis thaliana from the Chernobyl zone
Доповіді НАН України
description Tolerance to radiomimetics and heavy metals has been investigated in Arabidopsis thaliana plants collected in the Chernobyl zone. Decrease of root growth and DNA damage level of a single cell have been evaluated. Tolerance of A. thaliana from the zone to the growth on genotoxic medium has been revealed. It is noted that certain Arabidopsis plants recover genomic DNA faster than control ones collected outside of the zone. Screening revealed plant lines expressing various levels of tolerance to genotoxins.
format Article
author Shevchenko, G.V.
Talalaiev, O.S.
author_facet Shevchenko, G.V.
Talalaiev, O.S.
author_sort Shevchenko, G.V.
title Efficient mechanism of DNA repair stabilizes genome of Arabidopsis thaliana from the Chernobyl zone
title_short Efficient mechanism of DNA repair stabilizes genome of Arabidopsis thaliana from the Chernobyl zone
title_full Efficient mechanism of DNA repair stabilizes genome of Arabidopsis thaliana from the Chernobyl zone
title_fullStr Efficient mechanism of DNA repair stabilizes genome of Arabidopsis thaliana from the Chernobyl zone
title_full_unstemmed Efficient mechanism of DNA repair stabilizes genome of Arabidopsis thaliana from the Chernobyl zone
title_sort efficient mechanism of dna repair stabilizes genome of arabidopsis thaliana from the chernobyl zone
publisher Видавничий дім "Академперіодика" НАН України
publishDate 2017
topic_facet Біологія
url http://dspace.nbuv.gov.ua/handle/123456789/126564
citation_txt Efficient mechanism of DNA repair stabilizes genome of Arabidopsis thaliana from the Chernobyl zone / G.V. Shevchenko, O.S. Talalaiev // Доповіді Національної академії наук України. — 2017. — № 4. — С. 84-90. — Бібліогр.: 14 назв. — англ.
series Доповіді НАН України
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AT talalaievos efficientmechanismofdnarepairstabilizesgenomeofarabidopsisthalianafromthechernobylzone
first_indexed 2025-07-09T05:16:22Z
last_indexed 2025-07-09T05:16:22Z
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fulltext ISSN 1025-6415. Dopov. Nac. acad. nauk Ukr. 2017. № 484 ОПОВІДІ НАЦІОНАЛЬНОЇ АКАДЕМІЇ НАУК УКРАЇНИ БІОЛОГІЯ 30 years have passed since the explosion at the Chernobyl Nuclear Power Plant in 1986, and to- day, despite of heavy affection of radiation, flora is abundant even in the most contaminated sites of the Exclusion zone. This phenomenon proves plant adaptation to life in the chronic radiation environment and on soils polluted by heavy metals. Unfortunately, due to technological catas- trophes and devastating economics, there is a continuous contamination by heavy metals and radiation throughout the world, which affects animals and plants and threatens human health. At the same time, despite its notoriety, the Chernobyl area along with other anthropogenically contaminated places could be considered as the unique area allowing the investigation of genetic changes in organisms after a long-time impact of genotoxins. Such anthropogenically polluted areas provide scientific challenges to researches, and the outcome of the investigations will con- tribute significantly to the biotechnology of plant stress tolerance, soil remediation, and human health protection. Since plants cannot avoid environmental influences, their active vegetation in the Chernobyl zone evidences the application of extremely efficient mechanisms for minimizing harmful effects. As is known, radiation and heavy metals trigger a wide range of physiological and biochemical alterations causing DNA single- and double-strand breaks, and this results in the genome instabil- ity with potentially lethal consequences for the whole organism. During the first decade after the Chernobyl disaster (1986—1992), several studies have been performed to analyze plant genome © G.V. Shevchenko, O.S. Talalaiev, 2017 doi: https://doi.org/10.15407/dopovidi2017.04.084 UDC 577.215:582.683.2:621.311.25 G.V. Shevchenko, O.S. Talalaiev M.G. Kholodny Institute of Botany of the NAS of Ukraine, Kiev E-mail: g_shevchenko@botany.kiev.ua Efficient mechanism of DNA repair stabilizes genome of Arabidopsis thaliana from the Chernobyl zone Presented by Corresponding Member of the NAS of Ukraine E.L. Kordyum Tolerance to radiomimetics and heavy metals has been investigated in Arabidopsis thaliana plants collected in the Chernobyl zone. Decrease of root growth and DNA damage level of a single cell have been evaluated. Tolerance of A. thaliana from the zone to the growth on genotoxic medium has been revealed. It is noted that certain Arabidopsis plants recover genomic DNA faster than control ones collected outside of the zone. Screening revealed plant lines expressing various levels of tolerance to genotoxins. Keywords: DNA damage and repair, Arabidopsis thaliana roots, single cell DNA gel electrophoresis, Chernobyl. 85ISSN 1025-6415. Допов. Нац. акад. наук Укр. 2017. № 4 Efficient mechanism of DNA repair stabilizes genome of Arabidopsis thaliana from the Chernobyl zone changes in the Chernobyl zone [1, 2]. Along with chromosome aberrations in rye and wheat [3, 4] they revealed the increased occurrence of DNA single-strand breaks [1] and pronounced dose- dependent genome destabilization [2]. Studies of the frequency of somatic intrachromosomal homologous recombination events in Arabidopsis plants [2, 5] have shown its tendency to return to the control level during the sub- sequent years after the explosion [5], evidencing in this way that changes in plant genomes have important roles in the ongoing process of plant adaptation after a short period of radiation impact. Nevertherless, a lot of questions still remain about molecular mechanisms of genome stability and how it is preserved over a longer period. Therefore, further studies are clearly needed to predict the long-term impact of radioactive contaminations on plants. In connection with the above, we find it reasonable to check the adaptation ability of Arabi- dopsis plants from Chernobyl and to analyze to what extent the plant genome could be damaged and how fast it can be repaired. By this research, we would like to attract a wide attention of scientists and the social community to such an “open air laboratory” as the Chernobyl area and to promote investigations of the contamination impact on plants. Up-regulation of DNA repair is one of the possible mechanisms of adaptation. By this re- search, we will investigate how efficiently this mechanism is functioning in the areas contami- nated by radiation and heavy metals for thirty years. In particular, we investigated the ability of Arabidopsis to grow on a genotoxic medium (heavy metals and radiomimetics) and, in parallel, evaluated the kinetics of Arabidopsis genome recovery. For the latter task, we applied “comet as- say”, a versatile sensitive method for the evaluation of DNA damages and DNA repair capacity at a single-cell level [6]. The above method is used widely for checking the plant genome integrity after the influence of various toxins [7]. Our investigation has shown that Arabidopsis plants from Chernobyl tolerate the growth on a contaminated medium, and the genome of such plants is char- acterized by efficient repair capacity. Materials and methods. Seed collection and growth conditions. Native Arabidopsis plants have been collected in 2009—2015 from areas with different levels of contamination (from 0.5 to 9 μSv/h) around the Chernobyl nuclear power plant (village of Kopachi, towns of Yanov and Prypyat). Control plants were collected in the village of Koncha Zaspa (KZ), where the level of radiation was 0.15—0.20 μSv/h. Columbia0 strain was used as a laboratory control. Growth rate of Arabidopsis was assessed by germinating seeds for 10 days on square plastic plates with ½ MS medium including 0.5 % sucrose and cadmium chloride (CdCl2) in the con- centrations ranging from 100 to 300 μM (Fig. 1). Growth tests with bleomycin were performed with the application of a microphenotyping system [8], which is the extremely useful tool for screening a bulk of material. In the experi- Table 1. Sites of Arabidopsis plant collection in the Chernobyl zone Location of sites Doze of radiation Geographical coordinates 10 km Chernobyl Zone, Kopachi village ~0.7 μSv/h 51°20'59.3"N; 30°07'33.4"E 5 km Chernobyl Zone, town of Pryp'yat' ~5 μSv/h 51°24'04.1"N; 30°03'54.8"E 5 km Chernobyl Zone, town of Yanov ~15 μSv/h 51°23'29.2"N; 30°03'23.6"E Clean Control, Koncha Zaspa village, Kiev region ~0.20 μSv/h 50°29'N; 30°57'E 86 ISSN 1025-6415. Dopov. Nac. acad. nauk Ukr. 2017. № 4 G.V. Shevchenko, O.S. Talalaiev ments with growth inhibition, seeds were pre-germinated for 3 days on simple MS medium and then transferred to MS with bleomycin (1-7 μg/ml) for the next 3 days. Length of seedling roots has been measured by ImageJ programme (www.imagej.net), and the growth inhibition has been calculated using the formula I% = (μc − μt)/μc⋅100, where I% is the percentage of growth inhibi- tion, μc — mean value for root length in the control, and μt is value for root growth rate under the treatment. The average (from 100 plants for each type of treatment) size of the roots in cm is shown in Fig. 2. Comet assay method. DNA damage and kinetics of DNA recovery after treatment with radio- mimetic bleomycin are evaluated by the means of single cell DNA gel electrophoresis (SCGE) or so called “Comet assay” [7]. In particular, DNA-DSBs were detected by a neutral comet assay [7, 9]. For this, Arabidopsis seedlings were cut with a razor blade in phosphate-buffered saline (PBS, pH8), and nuclear suspension was dispersed in melted 0.7 % LMT (low melting point) agarose (15510-027, GibcoBRL, Gaithersburg, USA). Aliquots of LMT agarose with nuclear suspension were immediately pipetted onto each of two agarose coated microscope slides (two duplicates per slide). After solidification of agarose, the slides were put in a lysis solution (2.5 M NaCl, 10 mM Tris–HCl, 0.1 M EDTA, 1% N-lauroyl sarcosinate, pH 7.6) for at least 1 h to dissolve cellular membranes and to remove attached proteins. After lysis, electrophoresis was performed at 45V for 3 min. Then slides were dipped for 5 min in 70 % ethanol and 100% ethanol and air-dried. DNA ‘comets’ were visualized in epifluorescence with a Nikon Eclipse 800 microscope after staining with a SybrGold stain (Molecular-Probes Invitrogen, USA) and evaluated by the Comet module of the LUCIA cytogenetics software suite (LIM, Praha, Czech Republic). The fraction of DNA in comet tails (% of tail- DNA) was used as a measure of DNA damage. In Fig. 1. Decrease of root growth in seedlings of Arabi- dopsis plants from the Chernobyl zone grown on the MS medium with addition of Cd2+ (Col0 — Columbia, KZ — Arabidopsis plants from Kiev region, Chern1 and Chern2 — Arabidopsis plants from radioactively con- taminated sites) Fig. 2. Inhibition of root growth in Arabidopsis plants from the Chernobyl zone grown on a me- dium with addition of bleomycin Table 2. Time for fast DNA recovery accounting less than 60 % of damaged DNA A.thaliana plants Time of recovery, min Col0 3—5 KZ 10 Chern1 3—5 Chern2 3 87ISSN 1025-6415. Допов. Нац. акад. наук Укр. 2017. № 4 Efficient mechanism of DNA repair stabilizes genome of Arabidopsis thaliana from the Chernobyl zone each experiment, the % T DNA damage was measured at time points of 0, 3, 5, 10, 20, and 60 min after the treatment (1 h with 30 μg/ml bleomycin). Untreated plants were used as control. Mea- surements included four independent gel replicas of 25 evaluated comets (Fig. 3, 4). Repair time (tx) is defined as % damage remaining (tx) = mean % tail-DNA (tx) — mean % tail-DNA (control) mean % tail-DNA (t0) — mean % tail-DNA (control)* 100. Dose-response was calculated as the percentage of free DNA moved by electrophoresis into comet tail (% T DNA) (Fig. 3). Maximum damage is normalized as 100 % at t = 0 for all plants (Fig. 4).The rate of DSB repair was deter- mined by measuring the proportion of fragmented DNA at intervals during a recovery period (Fig. 3). Data on the time of DNA recovery is presented in table 2. Results and discussion. Root length measurements. Decrease of growth rate. In the set of experiments with tolerance to mutagens, we have tested approximately 10 Arabidopsis plants col- lected in the Chernobyl Exclusion zone. Control plants were collected from outside of the zone, where the level of radiation and soil contamination were negligible (Table 1). All plants expressed a dose-dependent decrease of growth (Fig. 1). Among Chernobyl samples, there were plants more and less tolerant to genotoxins (Chern1 and Chern2). In general, Arabidopsis from Chernobyl grew better on the medium with cadmium chloride (Fig. 1). Calculation of relative growth inhibition under growth on bleomycin also has shown its lower percentage in plants from Chernobyl (Fig. 2). Thus, the above results confirmed our previous investigations proving the tolerance of Arabi- dopsis from Chernobyl to the growth on a genotoxic medium [10]. Comet assay results. Our results have shown that both Arabidopsis plants grown in the Chernobyl zone and outside of it expressed the dose-dependent DNA fragmentation, and there was no significant difference in DNA damage by bleomycin between plants (Fig. 3, time point 0). Fig. 4. Kinetics of genome repair in various strains of Arabidopsis plants after treatment with bleomycin Fig. 3. DNA damage (%) in A. thaliana seedlings after treatment with bleomycin at the consecutive stages of genome recovery 88 ISSN 1025-6415. Dopov. Nac. acad. nauk Ukr. 2017. № 4 G.V. Shevchenko, O.S. Talalaiev All tested plants exhibited the sensitivity to genotoxin to a various extent. There were plants with significant tolerance to bleomycin, as well as more susceptible to it (Fig. 3). Starting from the first minutes of the plant recovery, there was a difference in the percent- age of DNA damage (Figs. 3, 4). Chernobyl plants expressed a more efficient repair capacity than the controls (Fig. 4). Moreover, the rates of DNA repair in all tested plants have a characteristic biphasic profile: an initial rapid phase (1—5 min) accounting for less than 60 % of the fragmented DNA followed by a slower phase (5—60 min) accounting for the remainder (Figs. 3, 4; Table 2). To our surprise, control plants (KZ) were even more susceptible to DNA damage than A.thaliana from Chernobyl and Col0 (Fig. 3). Thus, it becomes evident that plants collected in the Chernobyl zone have efficient and fast repair capacity. As is known, genetic responses to irradiation mainly depend on the efficiency of DNA repair systems in restoring the integrity of genome and preserving the heritable informa- tion. Our previous investigations on the sensitivity of Chernobyl plants to genotoxic agents have shown the increased expression of marker genes connected with such DNA repair pathways as ho- mologous recombination (HR) and non-homologous end joining (NHEJ) [10]. Both these DNA repair pathways are known to operate in plants [11—13], and molecular components of these pathways are highly conserved amongst eukaryotes [14]. Up-regulation of several key enzymes like CyclB1:1, Rad 54, and Ku80 suggests the involvement of both HR and NHEJ, as well as cell cycle regulation, in promoting plant genome stability in genotoxic environment of the Chernobyl zone [10]. Fast recovery has been noted also by Kozak et al. (2009) [13] in their research of Arabidopsis mutants providing evidence for a variation in the rate of DSB repair during the first 5—10 min. Basing on our experience and emerging works in this field, we must stress out that the comet assay method can be efficiently used for the rapid monitoring of genotoxicity induced in Arabi- dopsis seedlings. To sum up, we would like to note that the stability of Arabidopsis genome in the Chernobyl zone depends upon the efficient work of DNA damage repair mechanisms. It is worth to mention that the research of long term impact of radiation and heavy metals on plants in the nature has been performed for the first time, and it is a great contribution into our understanding of the ad- aptation ability of plants. In addition, it also provides knowledge for the biotechnology of stress tolerant plants. Acknowledgement. The investigation has been supported by Marie Curie Actions – Inter- national Research Staff Exchange Scheme (IRSES) grant № 612587. Many thanks to our col- laborators Prof. John Doonan (IBERS, Aberystwyth, UK) and Prof. Karel Angelis (Institute of Experimental Botany, Prague, Czech Republic) who supervised the experimental work with growth tests and comet assay. 89ISSN 1025-6415. Допов. Нац. акад. наук Укр. 2017. № 4 Efficient mechanism of DNA repair stabilizes genome of Arabidopsis thaliana from the Chernobyl zone REFERENCES 1. Syomov, A. B., Ptitsyna, S. N. & Sergeeva, S. A. (1992). Analysis of DNA strand break induction and repair in plants from the vicinity of Chernobyl. Sci. Total. Environ., 112, pp. 1-8. 2. Kovalchuk, O., Kovalchuk, I., Arkhipov, A. Telyuk, P., Hohn, B. & Kovalchuk, L. (1998). The Allium cepa chromosome aberration test reliably measures genotoxicity of soils of inhabited areas in the Ukraine contaminated by the Chernobyl accident. Mutat. Res./Genetic Toxicology and Environmental Mutagenesis, 415, Iss. 1-2, pp. 47-57. doi: http://dx.doi.org/10.1016/S1383-5718(98)00053-9. 3. Ziablitskaia, E. I., Geras’kin, S. A., Udalova, A. A. & Spirin, E. V. (1996). An Analysis of the Genetic Sequelae of the Contamination of Winter Rye Crops by the Radioactive Fallout From the Chernobyl Atomic Electric Power Station. Radiats. Biol. Radioecol., 36, No. 4, pp. 498-505. 4. Kovalchuk, I., Molinier, J., Yao, Y., Arkhipov, A. & Kovalchuk, O. (2007). Transcriptome analysis reveals fundamental differences in plant response to acute and chronic exposure to ionizing radiation. Mutat. Res./Fundamental and Molecular Mechanisms of Mutagenesis, 624, Iss. 1-2, pp. 101-113. doi: https://doi. org/10.1016/j.mrfmmm.2007.04.009 5. Kovalchuk, I., Abramov, V., Pogribny, I. & Kovalchuk, O. (2004). Molecular Aspects of Plant Adaption to Life in the Chernobyl Zone. Plant. Physiol., 135, pp. 357-363. 6. Santos, C. L. V., Pourrut, B. & Ferreira de Oliveira, J. M. P. (2015). The use of comet assay in plant toxicology: recent advances. Front. Genet., 6. doi: https://doi.org/10.3389/fgene.2015.00216 7. Angelis, K. J., Duљinská, M. & Collins, A. R. (1999). Single cell gel electrophoresis: Detection of DNA damage at different levels of sensitivity. Electrophoresis, 20, Iss. 10, pp. 2133-2138. doi: https://doi.org/10.1002/ (SICI)1522-2683(19990701)20:10<2133::AID-ELPS2133>3.0.CO;2-Q 8. Forde, B. G. (2013). Glutamate signaling in roots. J. Exp. Bot., 65, No 2, pp. 779-787. doi: https://doi.org/ 10.1093/jxb/ert335 9. Olive, P. L. & Banath, J. P. (2006). The comet assay: a method to measure DNA damage in individual cells. Nat. Protoc., 1, pp. 23-29. 10. Shevchenko, G. V., Talaliev, A. S., Doonan, J. (2012). Arabidopsis thaliana seedlings from the Chernobyl NPP zone are tolerant to DNA-damaging agents. Dopov. Nac. akad. nauk Ukr., No. 12, pp. 157-162 (in Russian). 11. Puchta, H. J. (2005). The repair of double – strand breaks in plants: mechanisms and consequences for genome evolution. J. Exp. Bot., 56, Iss. 409, pp. 1-14. doi: https:// doi.org/10.1093/jxb/eri025 12. Pacher, P., Beckman, J. S. & Liaudet, L. (2007). Nitric Oxide and Peroxynitrite in Health and Disease. Physiol. Rev., 87, No. 1, pp. 315-424. doi: https://doi.org/10.1152/physrev.00029.2006 13. Kozak, J., West, C., White, C. E., da Costa-Nunes, J. A. & Angelis, K. J. (2009). Rapid repair of DNA double strand breaks in Arabidopsis thaliana is dependent on proteins involved in chromosome structure maintenance. DNA Repair, 8, Iss. 1, pp. 413-419. doi: https://doi.org/10.1016/j.dnarep.2008.11.012 14. Bray, C. M. & West, C. E. (2005). DNA repair mechanisms in plants: crucial sensors and effectors for the maintenance of genome integrity. New. Phytol., 168, pp. 511-528. doi: https://doi.org/10.1111/j.1469-8137. 2005.01548.xReceived 08.11.2016 Г.В. Шевченко, О.С. Талалаєв Інститут ботаніки ім. М.Г. Холодного НАН України, Київ E-mail: g_shevchenko@botany.kiev.ua ЕФЕКТИВНИЙ МЕХАНІЗМ ВІДНОВЛЕННЯ ДНК СТАБІЛІЗУЄ ГЕНОМ РОСЛИН ARABIDOPSIS THALIANA ІЗ ЧОРНОБИЛЬСКОЇ ЗОНИ Досліджено стійкість проростків Arabidopsis thaliana із зони Чорнобильської АЕС до дії мутагенів (радіо- міметиків і важких металів). Проаналізовано швидкість росту коренів і ступінь пошкоджень ДНК окремих клітин рослин. Виявлено стійкість росту A. thaliana із зони на генотоксичному середовищі і встановлено, що деякі рослини із зони ЧАЕС відновлюють свій геном швидше, ніж контрольні, зібрані поза зоною. За результатами скринінгу визначено рослини різного ступеня чутливості до дії генотоксинів. Ключові слова: пошкодження та відновлення ДНК, корені Arabidopsis thaliana, електрофорез ДНК окремої клітини, Чорнобиль. 90 ISSN 1025-6415. Dopov. Nac. acad. nauk Ukr. 2017. № 4 G.V. Shevchenko, O.S. Talalaiev Г.В. Шевченко, А.С. Талалаев Институт ботаники им. Н.Г. Холодного НАН Украины, Киев E-mail: g_shevchenko@botany.kiev.ua ЭФФЕКТИВНЫЙ МЕХАНИЗМ ВОССТАНОВЛЕНИЯ ДНК СТАБИЛИЗИРУЕТ ГЕНОМ РАСТЕНИЙ ARABIDOPSIS THALIANA ИЗ ЧЕРНОБЫЛЬСКОЙ ЗОНЫ Исследована устойчивость проростков Arabidopsis thaliana из зоны Чернобыльской АЭС к действию му- тагенов (радиомиметиков и тяжелых металлов). Проанализированы скорость роста корней и степень по- вреждения ДНК единичной клетки растений. Обнаружена устойчивость роста A. thaliana из зоны на ге- нотоксичных средах и установлено, что некоторые растения из зоны ЧАЭС восстанавливают свой геном быстрее, чем контрольные, собранные вне зоны. В результате скрининга определены растения различной степени толерантности к воздействию генотоксинов. Ключевые слова: повреждение и восстановление ДНК, корни Arabidopsis thaliana, электрофорез ДНК от- дельной клетки, Чернобыль.

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