Expression profile of nuclear receptors upon epstein — barr virus induced b cell transformation
Background: Infection of human B cells with Epstein—Barr virus (EBV) induces metabolic activation, morphological transformation, cell proliferation and eventual immortalization. Aim: To identify the nuclear receptors, which are the cellular interaction partners of EBNAs, that will help to elucidat...
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| Datum: | 2009 |
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| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | English |
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Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
2009
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| Schriftenreihe: | Experimental Oncology |
| Schlagworte: | |
| Online Zugang: | http://dspace.nbuv.gov.ua/handle/123456789/135700 |
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| Назва журналу: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Zitieren: | Expression profile of nuclear receptors upon epstein — barr virus induced b cell transformation / S.P. Yenamandra, A. Lundin1, V. Arulampalam, M. Yurchenko, S. Pettersson, G. Klein, E. Kashuba // Experimental Oncology. — 2009. — Т. 31, № 2. — С. 92–96. — Бібліогр.: 23 назв. — англ. |
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Digital Library of Periodicals of National Academy of Sciences of Ukraine| Zusammenfassung: | Background: Infection of human B cells with Epstein—Barr virus (EBV) induces metabolic activation, morphological transformation, cell
proliferation and eventual immortalization. Aim: To identify the nuclear receptors, which are the cellular interaction partners of EBNAs,
that will help to elucidate the mechanism of B cell transformation. Methods: We have compared the nuclear receptor profile in the naïve
and EBV-transformed B-lymphocytes, using TaqMan LDA microfluidic card technology. Results: Out of 48 nuclear receptor, 17 showed
differential expression at the mRNA level. The expression of 5 genes was elevated in EBV-transformed cells, whereas 12 genes were downregulated
in lymphoblastoid cells (LCLs). 7 genes were studied at the protein level; 2 genes were up regulated (Nr2F2 and RARA) and
4 genes were down regulated (ERB, NUR77, PPARG, and VDR) in LCLs. Conclusion: The nuclear receptor profiling on EBV infected
B cells showed alterations of nuclear receptors expression at both mRNA and protein levels compared with non infected peripheral blood
cells. Further analysis on a possible role of each nuclear receptor in EBV induced cell transformation should be performed. |
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