Assessment of the effect of wave device application on morphological changes in organs and cells of irradiated animals
Aim: To study the effect of the Device for wave influence on biological objects on the prevention of the development of acute radiation sickness and chronic radiation syndrome in vivo. Materials and Methods: The studies were performed on white rats irradiated at a dose of 8 Gy. The experimental grou...
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Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
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| Zitieren: | Assessment of the effect of wave device application on morphological changes in organs and cells of irradiated animals / V. Bebeshko, I. Homolyako, V. Grynchyshyn // Experimental Oncology. — 2017 — Т. 39, № 4. — С. 281–285. — Бібліогр.: 17 назв. — англ. |
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irk-123456789-1385872018-06-20T03:06:07Z Assessment of the effect of wave device application on morphological changes in organs and cells of irradiated animals Bebeshko, V. Homolyako, I. Grynchyshyn, V. Original contributions Aim: To study the effect of the Device for wave influence on biological objects on the prevention of the development of acute radiation sickness and chronic radiation syndrome in vivo. Materials and Methods: The studies were performed on white rats irradiated at a dose of 8 Gy. The experimental group of irradiated rats was treated with a wave Device (Patent of Ukraine No. 53568) once, for 2.5 min, 1.5 h after irradiation. Their organs were processed by standard histologic methods. Results: In the demagnetized rats, dystrophic changes in cells and tissues of liver, lungs, kidneys, brain, bone marrow and spleen were insignificant in 60 days compared to the control non-demagnetized group of animals. Conclusion: The Device reduced the magnetic charge of magneto-containing elements and their compounds in the organism of the irradiated animals, and decreased the formation of reactive oxygen species, which play a key role in the development of radiation-induced diseases. 2017 Article Assessment of the effect of wave device application on morphological changes in organs and cells of irradiated animals / V. Bebeshko, I. Homolyako, V. Grynchyshyn // Experimental Oncology. — 2017 — Т. 39, № 4. — С. 281–285. — Бібліогр.: 17 назв. — англ. 1812-9269 http://dspace.nbuv.gov.ua/handle/123456789/138587 en Experimental Oncology Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
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Original contributions Original contributions |
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Original contributions Original contributions Bebeshko, V. Homolyako, I. Grynchyshyn, V. Assessment of the effect of wave device application on morphological changes in organs and cells of irradiated animals Experimental Oncology |
| description |
Aim: To study the effect of the Device for wave influence on biological objects on the prevention of the development of acute radiation sickness and chronic radiation syndrome in vivo. Materials and Methods: The studies were performed on white rats irradiated at a dose of 8 Gy. The experimental group of irradiated rats was treated with a wave Device (Patent of Ukraine No. 53568) once, for 2.5 min, 1.5 h after irradiation. Their organs were processed by standard histologic methods. Results: In the demagnetized rats, dystrophic changes in cells and tissues of liver, lungs, kidneys, brain, bone marrow and spleen were insignificant in 60 days compared to the control non-demagnetized group of animals. Conclusion: The Device reduced the magnetic charge of magneto-containing elements and their compounds in the organism of the irradiated animals, and decreased the formation of reactive oxygen species, which play a key role in the development of radiation-induced diseases. |
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Article |
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Bebeshko, V. Homolyako, I. Grynchyshyn, V. |
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Bebeshko, V. Homolyako, I. Grynchyshyn, V. |
| author_sort |
Bebeshko, V. |
| title |
Assessment of the effect of wave device application on morphological changes in organs and cells of irradiated animals |
| title_short |
Assessment of the effect of wave device application on morphological changes in organs and cells of irradiated animals |
| title_full |
Assessment of the effect of wave device application on morphological changes in organs and cells of irradiated animals |
| title_fullStr |
Assessment of the effect of wave device application on morphological changes in organs and cells of irradiated animals |
| title_full_unstemmed |
Assessment of the effect of wave device application on morphological changes in organs and cells of irradiated animals |
| title_sort |
assessment of the effect of wave device application on morphological changes in organs and cells of irradiated animals |
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Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
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2017 |
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Original contributions |
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| citation_txt |
Assessment of the effect of wave device application on morphological changes in organs and cells of irradiated animals / V. Bebeshko, I. Homolyako, V. Grynchyshyn // Experimental Oncology. — 2017 — Т. 39, № 4. — С. 281–285. — Бібліогр.: 17 назв. — англ. |
| series |
Experimental Oncology |
| work_keys_str_mv |
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2025-07-10T06:07:24Z |
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2025-07-10T06:07:24Z |
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1837239000863080448 |
| fulltext |
Experimental Oncology 39, 281–285, 2017 (December) 281
ASSESSMENT OF THE EFFECT OF WAVE DEVICE APPLICATION
ON MORPHOLOGICAL CHANGES IN ORGANS AND CELLS
OF IRRADIATED ANIMALS
V. Bebeshko1,*, I. Homolyako2, V. Grynchyshyn1
1Institute of Clinical Radiology, National Center for Scientific Research of Radiation Medicine,
National Academy of Medical Sciences of Ukraine, Kyiv 02000, Ukraine
2O.O. Shalimov National Institute of Surgery and Transplantology, National Academy of Medical Sciences
of Ukraine, Kyiv 03680, Ukraine
Aim: To study the effect of the Device for wave influence on biological objects on the prevention of the development of acute radia-
tion sickness and chronic radiation syndrome in vivo. Materials and Methods: The studies were performed on white rats irradiated
at a dose of 8 Gy. The experimental group of irradiated rats was treated with a wave Device (Patent of Ukraine No. 53568) once,
for 2.5 min, 1.5 h after irradiation. Their organs were processed by standard histologic methods. Results: In the demagnetized rats,
dystrophic changes in cells and tissues of liver, lungs, kidneys, brain, bone marrow and spleen were insignificant in 60 days compared
to the control non-demagnetized group of animals. Conclusion: The Device reduced the magnetic charge of magneto-containing
elements and their compounds in the organism of the irradiated animals, and decreased the formation of reactive oxygen species,
which play a key role in the development of radiation-induced diseases.
Key Words: ionizing radiation, wave Device, magnetic charge, magneto-containing compounds, radiation-induced diseases.
In the development of cell radiation injury in the
dose range from 0.01 to 20 Sv and higher, an important
role is played by oxygen, because irradiation leads
to the formation of high concentrations of reactive
oxygen species (ROS) — nitric oxide, peroxynitrite,
primary lipid peroxidation products and toxic products
of oxidative adducts of DNA. ROS arise during the
process of radiation-induced hydrolysis, and begin
from the formation of nonstable particles free radicals
“carriers” of noncoupled electrons. They, actually,
conduct this reaction “by chain” [1–4]. The latter plays
the main role in the pathogenesis of radiation- induced
diseases (acute radiation sickness and chro nic radia-
tion syndrome), and their oncohematological (leuke-
mia, lymphoma, myelodysplastic syndromes, solid
tumors) [6–17] and pulmonary (pneumosclerosis)
complications and of more than 150 diseases, which
have in the base peroxide chain processes with the
formation of unstable active radicals-carriers of non-
coupled electrons.
Despite the essential achievements of radiobio-
logy and radiation medicine in diagnostics and treat-
ment of acute and chronic radiation syndromes [5–7]
and their complications, there is no effective remedy
or a Device to prevent the development of these dis-
eases. There is a lack of reliable treatment methods
as well.
Device, which is protected by patent № 53568,
operates from ~ 220 V, 50 Hz. The device emits
electromagnetic waves, which reduce the magnetic
charge of magnetized elements and their compounds
in irradiated animals and humans, resulting in de-
creased ROS formation in the body, which prevents
the development of diseases associated with the
effect of ROS.
The aim of this investigation was to assess possible
effect of the use of the patent Device for intracavital
influence on biological objects (Patent of Ukrainian
No. 53568, V. Grynchyshyn) in in vivo model.
MATERIALS AND METHODS
The investigation was performed on white inbred
rats (m = 250 g, n = 10) irradiated with a dose of 8 Gy.
The study was performed in accordance with the
ethical principles for experiments on animals. The ex-
perimental group of irradiated rats (n = 5) were treated
with a wave Device (Patent of Ukraine No. 53568) once
for 2.5 min, 1.5 h after irradiation. Control animals
(n = 5) received no treatment. 60 days after radiation
the animals were anesthesized. Their organs (liver,
kidney, brain, lung, heart, spleen, bone marrow) were
processed by standard histologic methods. The paraf-
fin slides were colored by hematoxylin and eosin. The
examination and photo documentation was carried
out using a BX-41 microscope (Olympus, Japan) with
a micro video system and the program Quick-photo
(Germany).
The changes in the organs were assessed by de-
scription and by a half-quantity method using visual-
analogy scale with the step of 1 unit from 0 to 3+. In the
spleen and bone marrow we calculated the quantity
of megakaryocytes as the generalized indicator of the
proliferative activity in 10 visual fields at high magni-
fication (× 400).
In the rat organs of both groups we revealed mor-
phological changes of different intensity and disper-
sion; the individual variability of these changes was
observed.
Submitted: October 12, 2017.
*Correspondence: E-mail: vg.bebeshko@gmail.com
Abbreviations used: MALT — mucoso-associated lymphoid tissue;
ROS — reactive oxygen species.
Exp Oncol 2017
39, 4, 281–285
282 Experimental Oncology 39, 281–285, 2017 (December)
RESULTS AND DISCUSSION
The rats liver showed considerable protein-
hydropic dystrophy, which in the control group attained
point 3+ with separate small areas of hepatocyte lysis
and intralobular necrosis. Dystrophic changes and
considerable nuclear polymorphism with the preva-
lence of small nuclei, the phenomenon of pyknosis
and apoptosis testified the pronounced intoxication
and hypoxia. Inflammatory infiltration was insignificant
and might be observed only in separate portal tracts,
it was accompanied by a pronounced reaction of the
liver macrophages.
Sometimes slight manifestations of pyle phlebitis
were seen. The results of a half-quantitative analysis
in general showed, that the degree of liver damage
in the control group exceeded significantly the main
group (54 vs 31 points, respectively). So, the intensity
of degenerative processes in the liver under the influ-
ence of the Device decreased by 1.43 times (Fig. 1, 2).
Fig. 1. The liver slide of the radiation-exposed animal: sig-
nificant protein-hydropic dystrophia with hepatocyte nuclear
polymorphism (a); the liver slide of irradiated animal treated
with the Device structure of liver is close to the normal one (b).
Hematoxylin-eosin, × 400
0
0.5
1
1.5
2
2.5
3
3.5
4
1 2
Fig. 2. The significance of dystrophia in the liver of the irradi-
ated animals (1) is much higher than in the liver of the irradiated
animals treated with the Device (2)
Visible enough was also the reaction of the spleen.
The ratio of white and red pulp in the control rats was
0.78 according to point scoring. The volume of red
pulp was higher than the volume of white pulp. The
quantity of megakaryocytes calculated in the 10 visual
fields in high magnification was 0.38. This is evidence
of low proliferative activity of lymphoid tissue. More-
over, the devastation, atrophy, edema of red pulp and
sinuses were observed.
The Device contribute to a slight change of the ratio
between white and red pulp volumes (0.76). The volume
of red pulp was higher than the volume of white pulp,
but the quantity of lymphoid cells increased, the index
of the quantity of megakaryocytes which amounted
to 0.84 also increased considerably, which testified
the intensification of proliferative activity of cells in the
lymphoid and haemopoetic tissue (Fig. 3, 4).
Fig. 3. The slide of the spleen of the irradiated animal: swelling
and atrophic changes in the red pulp, plethora of sinuses of the
red pulp (a); the slide of the spleen of the radiation-exposed
animal treated with the Device: the increase of the cellularity,
group of functionally retained megakaryocytes in the spleen (b).
Hematoxylin-eosin, × 400
0
0.2
0.4
0.6
0.8
1
1 2
Fig. 4. The increase of the quantity of retained megakaryocytes
in the spleen of the irradiated animals (1) and in spleen of the
radiation-exposed rats with the use of the Device (2)
The comparison of the state of the bone marrow
was also carried out by the description of megakaryo-
cyte quantity. In rats of the main group under the
influence of the Device, we saw a decreased quantity
of dilated plethoric capillaries, increased cellularity
and safety of bone marrow cells; the middle quantity
of megakaryocytes stayed fixed — 14.6 (control),
17.2 (the main group) (Fig. 5).
Experimental Oncology 39, 281–285, 2017 (December) 283
Fig. 5. The bone marrow of the radiation-exposed animals —
intensive vascularisation (a); the bone marrow of the radiation-
exposed animals after the use of the Device the increase
of cellularity, retained megakaryocytes (b). Hematoxylin-eosin,
× 100 (a), × 400 (b)
The distinguished features of the reaction of the
pulmonary tissue on the radiation and on the radia-
tion with the use of the Device were intrinsic and
obvious.
After irradiation in the rats we have observed the
focal pneumonia, focal seats of stress reaction di-
lated plethoric capillaries of the intra-alveolar septa,
extravasates, the seats of edema, the diffuse and seat
thickening of the intra-alveolar septa with histiocytic
and macrophagal infiltration. The intensity of these
changes varied from moderate to pronounced. Mu-
coso-associated lymphoid tissue (MALT) had signs
of atrophy and a decrease of activity as a result of the
decreased volume of lymphoid tissue and absence
of germinal centers (Fig. 6).
Fig. 6. The slides of the lungs of radiation-exposed animals:
alveolar septae are thickened, infiltrated with histiomacropha-
gal elements, capillaries are dilated (a); focus of clear dilata-
tion of capillaries with haemorrhage (b). Hematoxylin-eosin,
× 200 (a), × 400 (b)
The employment of the Device lead to a decrease
of lung damage. The focuses of pneumonia had a small
size and of insignificant intensity, and were revealed only
in some animals. In the majority of the observations the
lung had an ordinary structure with single areas of at-
electases, dystelectases and emphysema. MALT also
returned to normal. In some animals the perivascular
lymphoid infiltration, which is evidence of the delayed
hypersensitivity, was seen (Fig. 7, Fig. 8).The kidneys
of the rats from both groups showed similar chang-
es — insignificant dilatation and congestion of the
vessels, some hemorrhages. The injure of glomeruli
was represented by hyper cellularity and the collapse
of the capillaries. Tubules were normal or dilated, with
the focuses of protein-hydropic or sometimes fatty
dystrophy. These changes were more typical for the
control group.
The general alignment of glomeruli injure in control
group was 3.36, in the main group 1.99 (Fig. 9, 10).
The structure of heart muscle and the structure
of the brain in both groups were in normal range.
It’s necessary to pay attention to the following.
In the rats irradiated by the 8 Gy dose (control group)
after the start of the experiment in all the organs were
observed noticeable pathologic changes, “vascular
reaction” (dilatation of vessels, plethoric, stasis,
hemorrhage) and noticeable dystrophic changes.
After the use of the Device the intensity of the vas-
cular reactions and dystrophy decreased. In the liver
the results of a half-quantity appreciation of intensity
of protein-hydropic dystrophy and other manifesta-
tions of dystrophy decreased by 1.43 times. In the
Fig. 7. The slides of the lungs of the radiation-exposed animals
treated with the Device: focuses of the slight thickening of alveo-
lar septae, infiltration with histiomacrophagal elements, capillar-
ies are dilatation (a); structure of the lung is close to the normal
one (b). Hematoxylin-eosin, × 100 (a) and × 40 (b)
Fig. 8. MALT of the radiation-exposed animals with the signs
of reduction (a); MALT of the radiation-exposed animals treated
with the Device; significant hyperplasia, reactive changes, large
reactive centers (b). Hematoxylin-eosin, × 200
Fig. 9. The slide of the kidney of the radiation-exposed animal:
protein-hydropic dystrophy of the tubular epithelium, increase
of glomerulus cellularity, the collapse of glomerular capillaries.
Hematoxylin-eosin, × 100
284 Experimental Oncology 39, 281–285, 2017 (December)
spleen the alignment of the white and red pulp in the
control and the main group had no significant dif-
ference (0.78 and 0.76, accordingly). But the index
of the quantity of megakaryocytes increased from
0.38 to 0.84. It reflects the essential intensification
of the proliferative reaction in the lymphoid and hemo-
poietic organs. Also, we could see the normalization
of structure of the red pulp.
The significant changes in the bone marrow were
observed. In the control group, mainly “fatty” bone
marrow was observed with middle intensity pathologic
changes in the elements of granulocytes and thrombo-
cytes lineage. In the bone marrow of the main group,
the restoration of the cells of these lineages and the
cells of erythroid lineage was higher in the “niche” (3+),
the intercellular connections were not destroyed and
the morphologic characteristics corresponded to the
normal state in intact rats. The quantity of megakaryo-
cytes in the marrow in the main group was sometimes
higher (17.2 vs 14.6 in control).
In the kidneys of the rats of the main (experimental)
group the parenchyma damage was also not consider-
able. The middle ratio between damaged and intact
glomeruli in the control group was 3.36, in the main
group — 1.99. It means that in the main group the
quantity of the damaged glomeruli was significantly
lower. Moreover, the signs of the protein-hydropic and
fatty dystrophy in the tubular epithelium and vascular
reactions were absent. The distinguishing features
of the lung reactions to the irradiation and on the ir-
radiation with the using of the Device are significant.
In the animals after the irradiation we could see the
centers of pneumonia, the manifestations of stress
reaction hemorrhage, delatation of capillaries of the
interalveolar barrier, extravasates, edema, diffuse and
the local thickening of the inter-alveolary barrier with
the infiltration of hystiocytes and makrophages.
The use of the Device led to a significant decrease
of lung damage and in most observations the lung
showed the normal structure with the single focuses
of atelectases, dystelectases, and emphysema. These
changes might be a concern to the ordinary function
of the lung or to the methods of avtanasia.
In irradiated animals MALT had the significant signs
of the reduction and decrease of activity: the decrease
of the volume of lymphoid tissue, absence of germina-
tive centers. In the main group, the normal structure
of MALT was seen. Some animals demonstrated
perivascular lymphoid infiltration — the manifestation
of the delayed hypersensitivity. The structure of the
heart muscle and structure of the brain in the both
groups were in the limits of normal deviations.
In summary, experimental study demonstrated
that Device for wave influence on biological objects
decreased the development of pathological processes
and, consequently, affected morphological changes
in the most radiosensitive organs (bone marrow,
spleen, lungs, etc.) in experimental animals.
The results of our investigations allow to re-
commend the Device for use by the workers in the
nuclear industry, participants of the liquidation of the
consequences of a techno-radiation accidents, radi-
ologists, radiobiologists and other workers, who work
with magnetic irradiators to prevent the development
of radiation-induced diseases and their complica-
tions. We consider that this wave device might to use
it in the treatment of oncohaematological diseases
that are treated using radiation and cytostatic drugs,
which increase the concentration of ROS in the body
of patients, the sources of disease recurrence and the
development of secondary tumors.
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Fig. 10. The rate of damaged glomeruli in the kidney of the
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