Peculiarities of DNA damage caused by exogenous nitric oxide combined with fractionated low dose ionizing radiation in normal and tumor cells

Peculiarities of DNA damage caused by exogenous nitric oxide combined with fractionated low dose ionizing radiation in normal and tumor cells

The aim of this study was to investigate the reaction of normal and tumor cells to genotoxic effect of widespread environmental factors — exogenous nitric oxides and ionizing radiation. Methods: The animals were treated with NO (125 mg/m3) and low dose ioni­zing radiation (10 acute exposures with 0....

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Datum:2015
Hauptverfasser: Muzalov, I.I., Mikhailenko, V.M.
Format: Artikel
Sprache:English
Veröffentlicht: Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України 2015
Schriftenreihe:Experimental Oncology
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Original contributions
Online Zugang:http://dspace.nbuv.gov.ua/handle/123456789/145453
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Назва журналу:Digital Library of Periodicals of National Academy of Sciences of Ukraine
Zitieren:Peculiarities of DNA damage caused by exogenous nitric oxide combined with fractionated low dose ionizing radiation in normal and tumor cells / I.I. Muzalov, V.M. Mikhailenko // Experimental Oncology. — 2015. — Т. 37, № 1. — С. 40-43. — Бібліогр.: 24 назв. — англ.

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spelling irk-123456789-1454532019-01-22T01:24:09Z Peculiarities of DNA damage caused by exogenous nitric oxide combined with fractionated low dose ionizing radiation in normal and tumor cells Muzalov, I.I. Mikhailenko, V.M. Original contributions The aim of this study was to investigate the reaction of normal and tumor cells to genotoxic effect of widespread environmental factors — exogenous nitric oxides and ionizing radiation. Methods: The animals were treated with NO (125 mg/m3) and low dose ioni­zing radiation (10 acute exposures with 0.1 Gy each). Genotoxicity was estimated in vivo in rats peripheral blood lymphocytes, bone marrow cells and tumor cells of Guerin carcinoma. DNA damages were assessed by alkaline single-cell gel electrophoresis. Results: Exogenous nitric oxides as well as irradiation caused significant increase of DNA damage in all types of investigated cells. The genotoxic effect increased in the order: peripheral blood lymphocytes < bone marrow cells < Guerin carcinoma cells. The greatest genotoxic effect was registered in Guerin carcinoma cells on terminal phase of tumor growth in rats exposed to NO and low dose ionizing radiation. Conclusions: Long-term exposure to common environmental factors (exogenous nitric oxides and ionizing radiation) capable to induce DNA damage in diffe­rent cells. Severity of the genotoxic effect depends on cell type and nature of impacting factors. NO caused more significant DNA damage than low dose ionizing radiation but the highest level of DNA damage was observed after their joint action. Obtained results confirm the real threat of cancer risk increase under combined action of common environmental factors of different nature. Key Words: nitric oxide, nitrosative stress, ionizing radiation, tumor cells, DNA damage. 2015 Article Peculiarities of DNA damage caused by exogenous nitric oxide combined with fractionated low dose ionizing radiation in normal and tumor cells / I.I. Muzalov, V.M. Mikhailenko // Experimental Oncology. — 2015. — Т. 37, № 1. — С. 40-43. — Бібліогр.: 24 назв. — англ. 1812-9269 http://dspace.nbuv.gov.ua/handle/123456789/145453 en Experimental Oncology Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
institution Digital Library of Periodicals of National Academy of Sciences of Ukraine
collection DSpace DC
language English
topic Original contributions
Original contributions
spellingShingle Original contributions
Original contributions
Muzalov, I.I.
Mikhailenko, V.M.
Peculiarities of DNA damage caused by exogenous nitric oxide combined with fractionated low dose ionizing radiation in normal and tumor cells
Experimental Oncology
description The aim of this study was to investigate the reaction of normal and tumor cells to genotoxic effect of widespread environmental factors — exogenous nitric oxides and ionizing radiation. Methods: The animals were treated with NO (125 mg/m3) and low dose ioni­zing radiation (10 acute exposures with 0.1 Gy each). Genotoxicity was estimated in vivo in rats peripheral blood lymphocytes, bone marrow cells and tumor cells of Guerin carcinoma. DNA damages were assessed by alkaline single-cell gel electrophoresis. Results: Exogenous nitric oxides as well as irradiation caused significant increase of DNA damage in all types of investigated cells. The genotoxic effect increased in the order: peripheral blood lymphocytes < bone marrow cells < Guerin carcinoma cells. The greatest genotoxic effect was registered in Guerin carcinoma cells on terminal phase of tumor growth in rats exposed to NO and low dose ionizing radiation. Conclusions: Long-term exposure to common environmental factors (exogenous nitric oxides and ionizing radiation) capable to induce DNA damage in diffe­rent cells. Severity of the genotoxic effect depends on cell type and nature of impacting factors. NO caused more significant DNA damage than low dose ionizing radiation but the highest level of DNA damage was observed after their joint action. Obtained results confirm the real threat of cancer risk increase under combined action of common environmental factors of different nature. Key Words: nitric oxide, nitrosative stress, ionizing radiation, tumor cells, DNA damage.
format Article
author Muzalov, I.I.
Mikhailenko, V.M.
author_facet Muzalov, I.I.
Mikhailenko, V.M.
author_sort Muzalov, I.I.
title Peculiarities of DNA damage caused by exogenous nitric oxide combined with fractionated low dose ionizing radiation in normal and tumor cells
title_short Peculiarities of DNA damage caused by exogenous nitric oxide combined with fractionated low dose ionizing radiation in normal and tumor cells
title_full Peculiarities of DNA damage caused by exogenous nitric oxide combined with fractionated low dose ionizing radiation in normal and tumor cells
title_fullStr Peculiarities of DNA damage caused by exogenous nitric oxide combined with fractionated low dose ionizing radiation in normal and tumor cells
title_full_unstemmed Peculiarities of DNA damage caused by exogenous nitric oxide combined with fractionated low dose ionizing radiation in normal and tumor cells
title_sort peculiarities of dna damage caused by exogenous nitric oxide combined with fractionated low dose ionizing radiation in normal and tumor cells
publisher Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
publishDate 2015
topic_facet Original contributions
url http://dspace.nbuv.gov.ua/handle/123456789/145453
citation_txt Peculiarities of DNA damage caused by exogenous nitric oxide combined with fractionated low dose ionizing radiation in normal and tumor cells / I.I. Muzalov, V.M. Mikhailenko // Experimental Oncology. — 2015. — Т. 37, № 1. — С. 40-43. — Бібліогр.: 24 назв. — англ.
series Experimental Oncology
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AT mikhailenkovm peculiaritiesofdnadamagecausedbyexogenousnitricoxidecombinedwithfractionatedlowdoseionizingradiationinnormalandtumorcells
first_indexed 2025-07-10T21:45:34Z
last_indexed 2025-07-10T21:45:34Z
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