FOXP3 gene promoter methylation in endometrial cancer cells

FOXP3 gene promoter methylation in endometrial cancer cells

Aim: To determine the methylation level of promoter region of the FOXP3 gene promoter depending on the heterogeneity of intracellular localization of its protein product in endometrial cancer (EC) cells and assess its relation to the clinical and morphological features of tumor. Materials and Method...

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Datum:2015
Hauptverfasser: Buchynska, L.G., Iurchenko, N.P., Verko, N.P., Nekrasov, K.A., Kashuba, V.I.
Format: Artikel
Sprache:English
Veröffentlicht: Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України 2015
Schriftenreihe:Experimental Oncology
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Original contributions
Online Zugang:http://dspace.nbuv.gov.ua/handle/123456789/145541
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Назва журналу:Digital Library of Periodicals of National Academy of Sciences of Ukraine
Zitieren:FOXP3 gene promoter methylation in endometrial cancer cells / L.G. Buchynska, N.P. Iurchenko, N.P. Verko, K.A. Nekrasov, V.I. Kashuba // Experimental Oncology. — 2015. — Т. 37, № 4. — С. 246-249. — Бібліогр.: 23 назв. — англ.

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spelling irk-123456789-1455412019-01-23T01:24:02Z FOXP3 gene promoter methylation in endometrial cancer cells Buchynska, L.G. Iurchenko, N.P. Verko, N.P. Nekrasov, K.A. Kashuba, V.I. Original contributions Aim: To determine the methylation level of promoter region of the FOXP3 gene promoter depending on the heterogeneity of intracellular localization of its protein product in endometrial cancer (EC) cells and assess its relation to the clinical and morphological features of tumor. Materials and Methods: Samples of surgical material of 40 EC patients who have not received any specific treatment before the surgery, were studied. Real time methylation-specific PCR (MSP) as well as morphological and immunohistochemical methods were used in the study. Results: Methylation of promoter region of the FOXP3 gene was determined in all EC cases, but variability of the methylation level in EC cells from 45.0% to 85.0% was observed. With tumor progression and in tumors with deep (≥ 1/2) invasion in myometrium, an increase of the methylation level of the FOXP3 and of cell number with cytoplasmic FOXP3 localization was observed. In EC patients the correlation between of methylation level of the FOXP3 gene and the number of FOXP3+ tumor cells with cytoplasmic expression (r = 0.41) was determined. Conclusion: The methylation level of FOXP3 gene promoter region and intracellular localization of its protein product are associated with tumor differentiation grade and the depth of myometrial invasion. 2015 Article FOXP3 gene promoter methylation in endometrial cancer cells / L.G. Buchynska, N.P. Iurchenko, N.P. Verko, K.A. Nekrasov, V.I. Kashuba // Experimental Oncology. — 2015. — Т. 37, № 4. — С. 246-249. — Бібліогр.: 23 назв. — англ. 1812-9269 http://dspace.nbuv.gov.ua/handle/123456789/145541 en Experimental Oncology Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
institution Digital Library of Periodicals of National Academy of Sciences of Ukraine
collection DSpace DC
language English
topic Original contributions
Original contributions
spellingShingle Original contributions
Original contributions
Buchynska, L.G.
Iurchenko, N.P.
Verko, N.P.
Nekrasov, K.A.
Kashuba, V.I.
FOXP3 gene promoter methylation in endometrial cancer cells
Experimental Oncology
description Aim: To determine the methylation level of promoter region of the FOXP3 gene promoter depending on the heterogeneity of intracellular localization of its protein product in endometrial cancer (EC) cells and assess its relation to the clinical and morphological features of tumor. Materials and Methods: Samples of surgical material of 40 EC patients who have not received any specific treatment before the surgery, were studied. Real time methylation-specific PCR (MSP) as well as morphological and immunohistochemical methods were used in the study. Results: Methylation of promoter region of the FOXP3 gene was determined in all EC cases, but variability of the methylation level in EC cells from 45.0% to 85.0% was observed. With tumor progression and in tumors with deep (≥ 1/2) invasion in myometrium, an increase of the methylation level of the FOXP3 and of cell number with cytoplasmic FOXP3 localization was observed. In EC patients the correlation between of methylation level of the FOXP3 gene and the number of FOXP3+ tumor cells with cytoplasmic expression (r = 0.41) was determined. Conclusion: The methylation level of FOXP3 gene promoter region and intracellular localization of its protein product are associated with tumor differentiation grade and the depth of myometrial invasion.
format Article
author Buchynska, L.G.
Iurchenko, N.P.
Verko, N.P.
Nekrasov, K.A.
Kashuba, V.I.
author_facet Buchynska, L.G.
Iurchenko, N.P.
Verko, N.P.
Nekrasov, K.A.
Kashuba, V.I.
author_sort Buchynska, L.G.
title FOXP3 gene promoter methylation in endometrial cancer cells
title_short FOXP3 gene promoter methylation in endometrial cancer cells
title_full FOXP3 gene promoter methylation in endometrial cancer cells
title_fullStr FOXP3 gene promoter methylation in endometrial cancer cells
title_full_unstemmed FOXP3 gene promoter methylation in endometrial cancer cells
title_sort foxp3 gene promoter methylation in endometrial cancer cells
publisher Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
publishDate 2015
topic_facet Original contributions
url http://dspace.nbuv.gov.ua/handle/123456789/145541
citation_txt FOXP3 gene promoter methylation in endometrial cancer cells / L.G. Buchynska, N.P. Iurchenko, N.P. Verko, K.A. Nekrasov, V.I. Kashuba // Experimental Oncology. — 2015. — Т. 37, № 4. — С. 246-249. — Бібліогр.: 23 назв. — англ.
series Experimental Oncology
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AT verkonp foxp3genepromotermethylationinendometrialcancercells
AT nekrasovka foxp3genepromotermethylationinendometrialcancercells
AT kashubavi foxp3genepromotermethylationinendometrialcancercells
first_indexed 2025-07-10T21:55:36Z
last_indexed 2025-07-10T21:55:36Z
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