Antinociceptive Effects of Valeriana Extract in Mice: Involvement of the Dopaminergic and Serotonergic Systems
Valeriana officinalis has been extensively used as a herbal remedy in traditional medicine. However, there is no clear evidence on the antinociceptive effects of this plant. The aim of our study was to evaluate the effect of Valeriana officinalis hydroalcoholic extract on pain modulation and i...
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Інститут фізіології ім. О.О. Богомольця НАН України
2013
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| Цитувати: | Antinociceptive Effects of Valeriana Extract in Mice: Involvement of the Dopaminergic and Serotonergic Systems / S. Shahidi, A. Bathaei, P. Pahlevani // Нейрофизиология. — 2013. — Т. 45, № 6. — С. 544-548. — Бібліогр.: 28 назв. — англ. |
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irk-123456789-1482452019-02-18T01:25:20Z Antinociceptive Effects of Valeriana Extract in Mice: Involvement of the Dopaminergic and Serotonergic Systems Shahidi, S. Bathaei, A. Pahlevani, P. Valeriana officinalis has been extensively used as a herbal remedy in traditional medicine. However, there is no clear evidence on the antinociceptive effects of this plant. The aim of our study was to evaluate the effect of Valeriana officinalis hydroalcoholic extract on pain modulation and its possible mechanism in mice. Adult male Balb/c mice were randomly divided into nine experimental groups. They received i.p. injections of saline, hydroalcoholic root extract of Valeriana officinalis (800, 200, or 50 mg/kg), and morphine; four groups received Valeriana (800 mg/kg) + antagonists of the systems involved in antinociception effects, naloxone, ondansetron, metoclopramide, or scopolamine. Tail-flick and writhing tests were used for estimation of possible modulation of pain. The tail-flick latencies in the Valeriana 800 and 200 mg/kg, but not 50 mg/kg, morphine, and combined Valeriana 800 + naloxone, ondansetron, metoclopramide, or scopolamine-treated groups were significantly longer than that in the control group. However, the tail-flick latencies in the Valeriana 800 mg/kg + ondansetron- and metoclopramide-treated groups were significantly shorter than that upon single action of the extract (800 mg/kg). The numbers of writhings in the extract-treated groups were smaller than in the control one. The numbers of writhings in the Valeriana (800 mg/kg)+ ondansetron- and metoclopramide-treated groups were significantly greater than in the extract (800 mg/kg) group. It is concluded that Valeriana officinalis extract possesses a clear analgesic effect and works through the serotonergic and dopaminergic systems. Валеріана лікувальна (Valeriana officinalis) широко використовується як рослинний лікувальний засіб у традиційній медицині. Достовірні відомості про можливу антиноцицептивну дію цієї рослини, проте, були відсутні. Ціллю нашого дослідження було оцінити в експериментах на мишах, чи здатний водноспиртовий екстракт валеріани модулювати біль, а також з’ясувати можливі механізми таких ефектів. Дорослі самці мишей лінії Balb/c були рандомізовано поділені на дев’ять груп. Їм внутрішньоочеревинно ін’єкували фізіологічний розчин, водноспиртовий екстракт з коренів валеріани (800, 200 або 50 мг сухої речовини на 1 кг маси), морфін, а в чотирьох групах – комбінації 800 мг/кг екстракту валеріани з антагоністами систем, залучених у реалізацію антиноцицептивних ефектів, – налоксоном, ондасетроном, метоклопрамідом або скополаміном. Для оцінки можливої модуляції болю використовували тест відсмикування хвоста та оцтовокислотний тест „корчів”. Латентні періоди відсмикування хвоста в групах, що отримували 800 та 200 (але не 50) мг екстракту та комбіновані ін’єкції 800 мг/кг валеріани із вказаними блокаторами, були довшими, ніж у групі контролю. В той же час латентні періоди цієї реакції в групах 800 мг/кг екстракту + ондасетрон та метоксипрамід були вірогідно меншими, ніж при ізольованій дії 800 мг/кг екстракту. Кількість „корчів” у мишей у межах періоду спостереження у відповідному тесті після ін’єкції екстракту була меншою, ніж у контролі. Число таких „корчів” у групах 800 мг/кг екстракту в комбінаціях із ондасетроном та метоклопрамідом було вірогідно більшим порівняно з тим, що спостерігалось у разі дії лише екстракту в аналогічної кількості. Зроблено висновок, що екстракт валеріани здійснює очевидний аналгетичний вплив, опосередкований серотонін- та дофамінергічною системами. 2013 Article Antinociceptive Effects of Valeriana Extract in Mice: Involvement of the Dopaminergic and Serotonergic Systems / S. Shahidi, A. Bathaei, P. Pahlevani // Нейрофизиология. — 2013. — Т. 45, № 6. — С. 544-548. — Бібліогр.: 28 назв. — англ. 0028-2561 http://dspace.nbuv.gov.ua/handle/123456789/148245 612.884 en Нейрофизиология Інститут фізіології ім. О.О. Богомольця НАН України |
| institution |
Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| collection |
DSpace DC |
| language |
English |
| description |
Valeriana officinalis has been extensively used as a herbal remedy in traditional medicine.
However, there is no clear evidence on the antinociceptive effects of this plant. The aim of
our study was to evaluate the effect of Valeriana officinalis hydroalcoholic extract on pain
modulation and its possible mechanism in mice. Adult male Balb/c mice were randomly
divided into nine experimental groups. They received i.p. injections of saline, hydroalcoholic
root extract of Valeriana officinalis (800, 200, or 50 mg/kg), and morphine; four groups
received Valeriana (800 mg/kg) + antagonists of the systems involved in antinociception
effects, naloxone, ondansetron, metoclopramide, or scopolamine. Tail-flick and writhing
tests were used for estimation of possible modulation of pain. The tail-flick latencies in the
Valeriana 800 and 200 mg/kg, but not 50 mg/kg, morphine, and combined Valeriana 800 + naloxone, ondansetron, metoclopramide, or scopolamine-treated groups were significantly
longer than that in the control group. However, the tail-flick latencies in the Valeriana 800
mg/kg + ondansetron- and metoclopramide-treated groups were significantly shorter than that
upon single action of the extract (800 mg/kg). The numbers of writhings in the extract-treated
groups were smaller than in the control one. The numbers of writhings in the Valeriana
(800 mg/kg)+ ondansetron- and metoclopramide-treated groups were significantly greater than
in the extract (800 mg/kg) group. It is concluded that Valeriana officinalis extract possesses a
clear analgesic effect and works through the serotonergic and dopaminergic systems. |
| format |
Article |
| author |
Shahidi, S. Bathaei, A. Pahlevani, P. |
| spellingShingle |
Shahidi, S. Bathaei, A. Pahlevani, P. Antinociceptive Effects of Valeriana Extract in Mice: Involvement of the Dopaminergic and Serotonergic Systems Нейрофизиология |
| author_facet |
Shahidi, S. Bathaei, A. Pahlevani, P. |
| author_sort |
Shahidi, S. |
| title |
Antinociceptive Effects of Valeriana Extract in Mice: Involvement of the Dopaminergic and Serotonergic Systems |
| title_short |
Antinociceptive Effects of Valeriana Extract in Mice: Involvement of the Dopaminergic and Serotonergic Systems |
| title_full |
Antinociceptive Effects of Valeriana Extract in Mice: Involvement of the Dopaminergic and Serotonergic Systems |
| title_fullStr |
Antinociceptive Effects of Valeriana Extract in Mice: Involvement of the Dopaminergic and Serotonergic Systems |
| title_full_unstemmed |
Antinociceptive Effects of Valeriana Extract in Mice: Involvement of the Dopaminergic and Serotonergic Systems |
| title_sort |
antinociceptive effects of valeriana extract in mice: involvement of the dopaminergic and serotonergic systems |
| publisher |
Інститут фізіології ім. О.О. Богомольця НАН України |
| publishDate |
2013 |
| url |
http://dspace.nbuv.gov.ua/handle/123456789/148245 |
| citation_txt |
Antinociceptive Effects of Valeriana Extract in Mice: Involvement of the Dopaminergic and Serotonergic Systems / S. Shahidi, A. Bathaei, P. Pahlevani // Нейрофизиология. — 2013. — Т. 45, № 6. — С. 544-548. — Бібліогр.: 28 назв. — англ. |
| series |
Нейрофизиология |
| work_keys_str_mv |
AT shahidis antinociceptiveeffectsofvalerianaextractinmiceinvolvementofthedopaminergicandserotonergicsystems AT bathaeia antinociceptiveeffectsofvalerianaextractinmiceinvolvementofthedopaminergicandserotonergicsystems AT pahlevanip antinociceptiveeffectsofvalerianaextractinmiceinvolvementofthedopaminergicandserotonergicsystems |
| first_indexed |
2025-07-12T18:42:58Z |
| last_indexed |
2025-07-12T18:42:58Z |
| _version_ |
1837467854577860608 |
| fulltext |
NEUROPHYSIOLOGY / НЕЙРОФИЗИОЛОГИЯ.—2013.—T. 45, № 6544
UDC 612.884
S. SHAHIDI1, A. BATHAEI1, and P. PAHLEVANI1,2
ANTINOCICEPTIVE EFFECTS OF VALERIANA EXTRACT IN MICE:
INVOLVEMENT OF THE DOPAMINERGIC AND SEROTONERGIC SYSTEMS
Received June 26, 2013.
Valeriana officinalis has been extensively used as a herbal remedy in traditional medicine.
However, there is no clear evidence on the antinociceptive effects of this plant. The aim of
our study was to evaluate the effect of Valeriana officinalis hydroalcoholic extract on pain
modulation and its possible mechanism in mice. Adult male Balb/c mice were randomly
divided into nine experimental groups. They received i.p. injections of saline, hydroalcoholic
root extract of Valeriana officinalis (800, 200, or 50 mg/kg), and morphine; four groups
received Valeriana (800 mg/kg) + antagonists of the systems involved in antinociception
effects, naloxone, ondansetron, metoclopramide, or scopolamine. Tail-flick and writhing
tests were used for estimation of possible modulation of pain. The tail-flick latencies in the
Valeriana 800 and 200 mg/kg, but not 50 mg/kg, morphine, and combined Valeriana 800 +
+ naloxone, ondansetron, metoclopramide, or scopolamine-treated groups were significantly
longer than that in the control group. However, the tail-flick latencies in the Valeriana 800
mg/kg + ondansetron- and metoclopramide-treated groups were significantly shorter than that
upon single action of the extract (800 mg/kg). The numbers of writhings in the extract-treated
groups were smaller than in the control one. The numbers of writhings in the Valeriana
(800 mg/kg)+ ondansetron- and metoclopramide-treated groups were significantly greater than
in the extract (800 mg/kg) group. It is concluded that Valeriana officinalis extract possesses a
clear analgesic effect and works through the serotonergic and dopaminergic systems.
Keywords: Valeriana officinalis, tail-flick test, writhing test, flavonoids, pain, mice.
1 Neurophysiology Research Center, Hamadan University of Medical
Sciences, Hamadan, Iran.
2 School of Medicine, Hamadan University of Medical Sciences, Hamadan,
Iran.
Correspondence should be addressed to P. Pahlevani
(e-mail: pouyan.pahlevani@gmail.com).
INTRODUCTION
In recent years, there has been significant scientific progress
in the neurobiology of pain control and introduction of
the respective modern technologies. Nonetheless, we still
need new analgesic drugs with better efficacy and lesser
side effects. There are several approaches allowing us to
use metabolites and compounds derived from known and
novel plants to make these drugs [1].
Valeriana officinalis is a well-known plant growing
wild in America and Eurasia [2] and commonly used
in traditional medicine [3]. According to many studies,
Valeriana possesses sedative, anxiolytic, tranquilizing,
spasmolytic, and anticonvulsant effects [4-8]. The
practical lack of side effects and low price are features
that make Valeriana so common [9]. Valeriana , when
producing hypolocomotor and axiolytic-like effects in
rats, did not alter oxidative stress parameters in the CNS,
liver, and kidneys [10].
Alkaloids, lignan, flavonoids, GABA, and valerenic acid
are active compounds found in Valeriana officinalis extracts
[6, 11, 12]. According to a few investigations, flavonoids
exert antinociceptic effects, and Valeriana alkaloids are also
members of the analgesic complement family [1, 13, 14].
As we see, Valeriana has a number of positive effects on
the nervous system, but there are no direct studies related to
possible antinociceptive effects of this remedy. The aim of
our study was to estimate the analgesic activity of different
doses of the extract from this plant and also to discover
possible mechanisms underlying its action.
METHODS
Animals. Adult male Balb/c mice weighing 25-35 g were
obtained from the Iran Pasteur Institute, Tehran. They
were kept at a constant temperature of 22 ± 2°C with a
12/12-h light/dark cycle (lights on at 7:00 a.m). Food and
NEUROPHYSIOLOGY / НЕЙРОФИЗИОЛОГИЯ.—2013.—T. 45, № 6 545
ANTINOCICEPTIVE EFFECTS OF VALERIANA EXTRACT
water were available ad libitum in the home cages. The
animals were acclimated to the environment for 1 week
before the experimental procedure. All procedures and
experiments were performed between 10:00 and 14:00.
Herbal Material and Extraction. Dried roots of
Valeriana officinalis were purchased from the Herbal
Medicine Research Center in Hamadan, Iran. The
ground material was immersed in 70% ethanol for
72 h at room temperature. The extract was filtered and
then freezer-dried.
Tail-Flick Test. A tail-flick apparatus for mice was
used to produce radiant heat on the mouse tail (about 3 cm
from the tip). The heat intensity was adjusted such that
the mean tail-flick latency (TFL) was about 3 sec, and
a maximum cut-off time of 8 sec was set to avoid tissue
damage. Mice were randomly divided into nine groups
(n = 8 each). These groups received saline (control group),
dry Valeriana extract (800, 200, or 50 mg/kg) dissolved
in saline, morphine (1 mg/kg; positive antinociception
control group), and co-administrations of Valeriana
(800 mg/kg) with naloxone (a blocker of opioid
receptors, 4 mg/kg), ondansetron (an antagonist of
the serotonergic system, 0.5 mg/kg), metoclopramide
(an antagonist of the dopaminergic system,
1 mg/kg), or scopolamine (an antagonist of muscarinic
cholinoreceptors, 1 mg/kg). Three tail-flick trials (with
1-min-long intertrial intervals) were performed before
and after drug administration. The TLF for each rat
was calculated as the average of three consecutive
measurements.
Acetic Acid Writhing Test. This test is based on
chemical induction of visceral pain. Acetic acid (0.6% in
saline, 0.1 ml/10 g body mass) was administered by i.p
injections. Immediately after administration, each animal
was placed inside a transparent Plexiglas box. The number
of abdominal writhes accompanied by elongation of the
body and stretching of the hindlimbs was counted for
30 min. The experimental groups (n = 8 each) were treated
similarly to what was described for the tail-flick test.
Statistical Analysis. The Student’s paired t-test was
used for comparing the baseline and post-injection
TLFs. Statistical comparisons among experimental
groups were performed by one-way analysis of
variance (ANOVA) followed by the Tukey test. The
level of P < 0.05 was taken as significant. Data are
presented below as means ± s.e.m.
RESULTS
Tail-Flick Test. Statistical analysis using the paired
t-test revealed that i.p. injections of Valeriana
0
Contr. V800. V200. V50. Morph.. Nal+V800 Ond+V800 Met+V800 Scop+V800
1
2
3
4
5
6
7
8
sec
***
###
+
+++
+++
+
###
### ###
###
#
*** *** *** *** ****
F i g. 1. Changes in the tail-flick latency (vertical scale, sec, before and after treatment) in experimental groups. The animals were divided
into the following groups: control (Contr.), V800 (received 800 mg/kg of the extract of the Valeriana officinalis), V200 (200 mg/kg Valeriana),
V50 (50 mg/kg Valeriana), Morph. (1.0 mg/kg morphine), Nal + V800 (naloxone and 800 mg/kg Valeriana), Ond + V800 (ondansetron and
800 mg/kg Valeriana), Met + V800 (metoclopramide and 800 mg/kg Valeriana), and Scop + V800 (scopolamine and 800 mg/kg Valeriana).
*P < 0.05 and **P < 0.01, cases of significant differences of post-injection values vs. pre-injection ones in the same group. #P < 0.05, ##P < 0.01, and
###P < 0.001, significantly different compared to the control group. +P < 0.05, and +++P < 0.001, significantly different compared to the V800 group.
Each column represents means ± s.e.m.
Р и c. 1. Середні значення латентного періоду відсмикування хвоста (вертикальна шкала, с) в експериментальних групах перед
уведенням тестованих агентів та після цього.
NEUROPHYSIOLOGY / НЕЙРОФИЗИОЛОГИЯ.—2013.—T. 45, № 6546
S. SHAHIDI, A. BATHAEI, and P. PAHLEVANI
extract at doses of 200 and 800 mg/kg (but not of
50 mg/kg), morphine, and Valeriana (800 mg/kg)
with naloxone, ondansetron, metoclopramide, and
scopolamine significantly increased the TFL, as
compared with the pretreatment (Fig. 1). Comparison
of the TFL using one-way ANOVA indicated that
there were no significant differences between the
experimental groups before treatment (P > 0.05), but
the differences were highly significant after treatment
(P < 0.0001). The Tukey post-hoc test showed that the
TFL values showed no significant difference between the
Valeriana (800 mg/kg) and morphine groups, Valeriana
(800 mg/kg) + naloxone, and Valeriana (800 mg/kg) +
+ scopolamine. However, the TFL mean value in
the Valeriana (800 mg/kg) group was significantly
greater than TFLs in the groups that received
200 and 50 mg/kg of the Valeriana extract,
Valeriana (800 mg/kg) + ondansetron, and Valeriana
(800 mg/kg)+ metoclopramide (Fig. 1).
Writhing Test. Statistical analysis by one-
way ANOVA showed that there were significant
differences in the number of writhings between
animal groups (P < 0.0001). The Tukey post-
hoc test indicated that, except for the Valeriana
(50 mg/kg) group, the numbers of writhings in
other groups were significantly smaller than
those in the control group. Also, there were no
significant differences in the number of writhings
between the mouse groups treated with Valeriana
(800 mg/kg), Valeriana (800 mg/kg) + naloxone,
and Valer iana (800mg/kg) + scopolamine .
However, the mean numbers of writhings in the
two Valeriana-treated groups (50 and 200 mg/kg),
800 mg/kg Valeriana + ondansetron, and 800 mg/kg
Valeriana + metoclopramide were significantly
greater than those in mice that received only
800 mg/kg Valeriana (Fig. 2).
DISCUSSION
Progress in developing analgesic drugs has been rather
significant in recent years, but the need for drugs with
better effects remains. Herbal medicine and the use of
medicinal plants is one of the main ways to meet this
need, and more and more investigations are performed
in this field.
Valeriana ofiicinalis in Eurasia and America,
Valeriana wallichi in India, and Valeriana angustifolia
in China and Japan are species of Valeriana that
are extensively used for calming, sleep induction,
and sedative effects in different nations. Valeriana
decreases the general activity of the CNS via inhibiting
GABA breakdown in particular [9, 12, 15, 16].
Valeriana is also used for relieving anxiety and for
insomnia [17-19].
To our knowledge, the analgesic effects of this
plant have not been estimated directly until now.
Results of our study show that the hydroalcoholic
extract of Valeriana off icinalis demonstrates
s i gn i f i c an t dose -dependen t an t i noc i cep t i ve
effects. The highest (800 mg/kg) dose of the
extract successfully reduced somatic pain, while a
50 mg/kg dose did not. Ondansetron, a serotonergic
system antagonist, and metoclopramide, a dopaminergic
system antagonist, significantly decreased the effect
F i g. 2. The number of writhings (vertical
scale) during 30 min in the acetic acid-induced
writhing test. Experimental animals were
divided into the following groups: control
(Contr.), V800 (received 800 mg/kg Valeriana),
V200 (200 mg/kg Valeriana), V50 (50 mg/kg
Valeriana), Morph. (1.0 mg/kg morphine), Nal +
V800 (naloxone and 800 mg/kg Valeriana), Ond
+ V800 (ondansetron and 800 mg/kg Valeriana),
Met + V800 (metoclopramide and 800 mg/kg
Valeriana), and Scop + V800 (scopolamine and
800 mg/kg Valeriana). *P < 0.05, **P < 0.01
and ***P < 0.001, compared to the control group;
#P < 0.05, ##P < 0.01, and ###P < 0.001, compared
to the V800 group. Each column represents
means ± s.e.m.
Р и с. 2. Кількість „корчів” у межах
30-хвилинного періоду спостереження
(вертикальна шкала) в оцтовокислотному
тесті (індукція вісцерального болю).
0
10
20
30
40
50
60
70
Contr. V800. V200. V50. Morph.. Nal+
V800
Ond+
V800
Met+
V800
Scop+
V800
***
***
***
***
***
***
***
###
###
###
###
#
NEUROPHYSIOLOGY / НЕЙРОФИЗИОЛОГИЯ.—2013.—T. 45, № 6 547
ANTINOCICEPTIVE EFFECTS OF VALERIANA EXTRACT
of the extract, showing that Valeriana blocked pain
via its influence on the respective pathways. We can
say that ondansetron exerted a more intense blocking
effect compared to that of metoclopramide. We
found no significant difference between the animal
groups treated with Valeriana (800 mg/kg), Valeriana
(800 mg/kg) + naloxone, and Valeriana (800 mg/kg) +
+ scopolamine, indicating that the antinociception
effect of the extract is preserved, and that the
opioidergic and cholinergic systems are probably
not involved so significantly in the induction of
antinociceptic effects of the Valeriana extract.
The antinociceptive activity was also clearly
expressed as significant reduction in the number of
abdominal writhings when compared to that in control
animals. In this test, we obtained results comparable
with those in the tail-flick test. The numbers if
withings were reduced with increase in the dose of
the extract; thus, the dose dependence of the Valeriana
hydroalcoholic extract was also quite clear. In this test,
ondansetron and metoclopramide also antagonized the
effects of the extract.
Valeriana may affect the CNS by interaction with
the serotonin, GABA, melatonin, or adenosine systems
[20]. Long-term treatment with Valeraiana officinalis
exerted no effect on the reduction of dopamine uptake
in the striatum of Wistar rats [10]. Aqueous and
methanolic extracts of Valeriana adscendens (one of
the Valeriana species) showed affinity with respect
to D1 and 5-HT1A receptors, while no affinity with
respect to noradrenergic receptors has been reported
[21]. 6-Methylapigenin is a flavonoid detected in
Valeriana officinalis. This flavone is a ligand for a
benzodiazepine binding site in GABAA receptors and
was reported to cause anxiolytic effects in mice [22].
A flavonoid, 1-hydroxypinoresinol, derived from
Valeriana officinalis, is a ligand for 5-HT1A receptors
[23]; these receptors were found to be involved, with
high efficacy, in the central analgesia mechanism [24,
25]. Hesperidin, a flavonone glycoside, was found to
be present in Valeriana [11], and it was reported that
linarin, another flavonone glycoside, was also found in
this plant [26]. Clear sedation [27] and antinociception
[28] effects of hesperidin have been reported earlier.
Thus, the antinociception effects of Valeriana
officinalis have been assessed quite obviously. These
effects are significantly antagonized by ondansetron
and metoclopramide. Naloxone and scopolamine
exerted much smaller influences on the Valeriana
hypoalgezic effects in the tail-flick and writhing tests.
We can conclude that the antinociception effects of
this remedy are mediated by the serotonergic and
dopaminergic systems, and flavonoids affect the
latter. These pathways have not been reported for the
Valeriana officinalis effects earlier, and our results
may serve as a motivation for further investigations
in this field.
All procedures for the humane treatment of animals
were approved by the Research Committee of the Hamadan
University of Medical Sciences and were performed according
to the Guide for Care and Use of Laboratory Animals published
by the United States National Institutes of Health (NIH
Publication No. 85-23, revised 1985).
The authors, S. Shahidi, A. Bathaei, and P. Pahlevani, have
no conflict of interest.
This work was financially supported by a grant from the
Hamadan University of Medical Sciences.
С. Шахіді1, A. Батхаєї1, П. Пахлевані1,2
АНТИНОЦИЦЕПТИВНІ ЕФЕКТИ ЕКСТРАКТУ
ВАЛЕРІАНИ В ЕКСПЕРИМЕНТАХ НА МИШАХ:
ЗАЛУЧЕННЯ ДОФАМІН- ТА СЕРОТОНІНЕРГІЧНОЇ
СИСТЕМ
1 Нейрофізіологічний дослідницький центр Хамаданського
медичного університету (Іран).
2 Хамаданський медичний університет (Іран).
Р е з ю м е
Валеріана лікувальна (Valeriana officinalis) широко
використовується як рослинний лікувальний засіб
у традиційній медицині. Достовірні відомості про
можливу антиноцицептивну дію цієї рослини, проте,
були відсутні. Ціллю нашого дослідження було оцінити
в експериментах на мишах, чи здатний водноспиртовий
екстракт валеріани модулювати біль, а також з’ясувати
можливі механізми таких ефектів. Дорослі самці мишей
лінії Balb/c були рандомізовано поділені на дев’ять груп.
Їм внутрішньоочеревинно ін’єкували фізіологічний
розчин, водноспиртовий екстракт з коренів валеріани
(800, 200 або 50 мг сухої речовини на 1 кг маси), морфін,
а в чотирьох групах – комбінації 800 мг/кг екстракту
валеріани з антагоністами систем, залучених у реалізацію
антиноцицептивних ефектів, – налоксоном, ондасетроном,
метоклопрамідом або скополаміном. Для оцінки можливої
модуляції болю використовували тест відсмикування
хвоста та оцтовокислотний тест „корчів”. Латентні періоди
відсмикування хвоста в групах, що отримували 800 та 200
(але не 50) мг екстракту та комбіновані ін’єкції 800 мг/кг
валеріани із вказаними блокаторами, були довшими, ніж у
групі контролю. В той же час латентні періоди цієї реакції в
групах 800 мг/кг екстракту + ондасетрон та метоксипрамід
були вірогідно меншими, ніж при ізольованій дії 800 мг/кг
екстракту. Кількість „корчів” у мишей у межах періоду
NEUROPHYSIOLOGY / НЕЙРОФИЗИОЛОГИЯ.—2013.—T. 45, № 6548
S. SHAHIDI, A. BATHAEI, and P. PAHLEVANI
спостереження у відповідному тесті після ін’єкції екстракту
була меншою, ніж у контролі. Число таких „корчів” у групах
800 мг/кг екстракту в комбінаціях із ондасетроном та
метоклопрамідом було вірогідно більшим порівняно з тим,
що спостерігалось у разі дії лише екстракту в аналогічної
кількості. Зроблено висновок, що екстракт валеріани
здійснює очевидний аналгетичний вплив, опосередкований
серотонін- та дофамінергічною системами.
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