Selective cytotoxicity and modification activity of picornaviruses on transformed cell lines
Aim. We do analyze of the dynamics of morphometabolic changes in transformed cells (of susceptoible lines) demonstrating resistance to picrnaviral infection. Methods. The study was performed by application of cell culture technology and a complex of cytochemical and cytophotometric assays. Were us...
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| Datum: | 2011 |
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| Hauptverfasser: | , |
| Format: | Artikel |
| Sprache: | English |
| Veröffentlicht: |
Інститут молекулярної біології і генетики НАН України
2011
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| Schriftenreihe: | Вiopolymers and Cell |
| Online Zugang: | http://dspace.nbuv.gov.ua/handle/123456789/156377 |
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| Назва журналу: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Zitieren: | Selective cytotoxicity and modification activity of picornaviruses on transformed cell lines / Z.A. Karalyan, H.R. Avagyan // Вiopolymers and Cell. — 2011. — Т. 27, № 5. — С. 377-380. — Бібліогр.: 8 назв. — англ. |
Institution
Digital Library of Periodicals of National Academy of Sciences of Ukraine| Zusammenfassung: | Aim. We do analyze of the dynamics of morphometabolic changes in transformed cells (of susceptoible lines)
demonstrating resistance to picrnaviral infection. Methods. The study was performed by application of cell
culture technology and a complex of cytochemical and cytophotometric assays. Were used picornaviruses from
various genu. Results. According to the results obtained, resistant to picornavirus infection cells of different
susceptible lines have similar changes in the phenotype. They have decreased number of nucleoli and increased
percentage of euploidy (and near euploid). In resistant cells of all cultures the reduction in amount of DNA and
RNA both in nucleus and in cytoplasm wasfound. All these data correlated with the increased euploidy (and near
euploid) of the resistant population. All picornavirus resistant cells had a less transformed phenotype, and
decreased proliferative activity. Decreased nucleolar status becomes apparent by reduction of all nucleolar
indices. Conclusions. Picornaviruses on the susceptible cells produce 2 types of changes – selection and
modification. Whateverthe mechanism, it isspecific for an individual virus, since no restrictions occur in case of
infection caused by another picornavirus.
Keywords: picornavirus, euploidy, nucleus, nucleolus. |
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