Association of mitochondrial lysyl-tRNA synthetase with HIV-1 GagPol involves catalytic domain of the synthetase and transframe and integrase domains of Pol
Aim. Analyze the interaction between Lysyl-tRNA synthetase (LysRS) and HIV-1 GagPol to know whether a particular N-terminal sequence of mitochondrial LysRS triggers a specific recognition with GagPol. Methods. Yeast two-hybrid analysis, immunoprecipitation. Results. We have shown that LysRS associ...
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| Date: | 2011 |
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| Main Authors: | , , , , , , |
| Format: | Article |
| Language: | English |
| Published: |
Інститут молекулярної біології і генетики НАН України
2011
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| Series: | Вiopolymers and Cell |
| Online Access: | http://dspace.nbuv.gov.ua/handle/123456789/156406 |
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| Journal Title: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Cite this: | Association of mitochondrial lysyl-tRNA synthetase with HIV-1 GagPol involves catalytic domain of the synthetase and transframe and integrase domains of Pol / L. Kobbi, J. Dias, G. Octobre, M. Comisso, M. Kaminska, V.F. Shalak, M. Mirande // Вiopolymers and Cell. — 2011. — Т. 27, № 5. — С. 381-382. — Бібліогр.: 8 назв. — англ. |
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Digital Library of Periodicals of National Academy of Sciences of Ukraine| Summary: | Aim. Analyze the interaction between Lysyl-tRNA synthetase (LysRS) and HIV-1 GagPol to know whether a particular N-terminal sequence of mitochondrial LysRS triggers a specific recognition with GagPol. Methods.
Yeast two-hybrid analysis, immunoprecipitation. Results. We have shown that LysRS associates with the Pol domain of GagPol. Conclusions. A model of the assembly of the LysRS:tRNA₃Lys:GagPol packaging complex is
proposed.
Keywords: tRNA₃Lys, lysyl-tRNA synthetase, HIV-1, packaging. |
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