Association of mitochondrial lysyl-tRNA synthetase with HIV-1 GagPol involves catalytic domain of the synthetase and transframe and integrase domains of Pol
Aim. Analyze the interaction between Lysyl-tRNA synthetase (LysRS) and HIV-1 GagPol to know whether a particular N-terminal sequence of mitochondrial LysRS triggers a specific recognition with GagPol. Methods. Yeast two-hybrid analysis, immunoprecipitation. Results. We have shown that LysRS associ...
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Інститут молекулярної біології і генетики НАН України
2011
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| Schriftenreihe: | Вiopolymers and Cell |
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| Zitieren: | Association of mitochondrial lysyl-tRNA synthetase with HIV-1 GagPol involves catalytic domain of the synthetase and transframe and integrase domains of Pol / L. Kobbi, J. Dias, G. Octobre, M. Comisso, M. Kaminska, V.F. Shalak, M. Mirande // Вiopolymers and Cell. — 2011. — Т. 27, № 5. — С. 381-382. — Бібліогр.: 8 назв. — англ. |
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irk-123456789-1564062019-06-19T01:25:16Z Association of mitochondrial lysyl-tRNA synthetase with HIV-1 GagPol involves catalytic domain of the synthetase and transframe and integrase domains of Pol Kobbi, L. Dias, J. Octobre, G. Comisso, M. Kaminska, M. Shalak, V.F. Mirande, M. Aim. Analyze the interaction between Lysyl-tRNA synthetase (LysRS) and HIV-1 GagPol to know whether a particular N-terminal sequence of mitochondrial LysRS triggers a specific recognition with GagPol. Methods. Yeast two-hybrid analysis, immunoprecipitation. Results. We have shown that LysRS associates with the Pol domain of GagPol. Conclusions. A model of the assembly of the LysRS:tRNA₃Lys:GagPol packaging complex is proposed. Keywords: tRNA₃Lys, lysyl-tRNA synthetase, HIV-1, packaging. Мета. Встановити, чи може N-кінцева послідовність, яка є специфічною для мітохондріальної форми лізил-тРНК синтетази, забезпечувати взаємодію цього ферменту з білком GagPol ВІЛ-1. Методи. Двогібридна дріжджова система, імунопреципітація. Результати. Ми показали що лізил-тРНК синтетаза взаємодіє з доменом Pol білка Gag. Висновки. Запропоновано модель утворення комплексу ЛізРС:тРНК₃Ліз:GagPol. Ключові слова: тРНК₃Ліз, лізил-тРНК синтетаза, ВІЛ-1, збирання віріона. Цель. Выяснить, может ли N-концевая последовательность, являющаяся специфичной для митохондриальной формы лизилтРНК синтетазы, обеспечивать взаимодействие этого фермента с белком GagPol ВИЧ-1. Методы. Двугибридная дрожжевая система, иммунопреципитация Результаты. Мы показали, что лизил-тРНК синтетаза взаимодействует с доменом Pol белка Gag. Выводы. Предложена модель образования комплекса ЛизРС:тРНК₃Лиз:GagPol. Ключевые слова: тРНК₃Лиз, лизил-тРНК синтетаза, ВИЧ-1, сборка вириона. 2011 Article Association of mitochondrial lysyl-tRNA synthetase with HIV-1 GagPol involves catalytic domain of the synthetase and transframe and integrase domains of Pol / L. Kobbi, J. Dias, G. Octobre, M. Comisso, M. Kaminska, V.F. Shalak, M. Mirande // Вiopolymers and Cell. — 2011. — Т. 27, № 5. — С. 381-382. — Бібліогр.: 8 назв. — англ. 0233-7657 DOI: http://dx.doi.org/10.7124/bc.000128 http://dspace.nbuv.gov.ua/handle/123456789/156406 577.22 en Вiopolymers and Cell Інститут молекулярної біології і генетики НАН України |
| institution |
Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| collection |
DSpace DC |
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English |
| description |
Aim. Analyze the interaction between Lysyl-tRNA synthetase (LysRS) and HIV-1 GagPol to know whether a particular N-terminal sequence of mitochondrial LysRS triggers a specific recognition with GagPol. Methods.
Yeast two-hybrid analysis, immunoprecipitation. Results. We have shown that LysRS associates with the Pol domain of GagPol. Conclusions. A model of the assembly of the LysRS:tRNA₃Lys:GagPol packaging complex is
proposed.
Keywords: tRNA₃Lys, lysyl-tRNA synthetase, HIV-1, packaging. |
| format |
Article |
| author |
Kobbi, L. Dias, J. Octobre, G. Comisso, M. Kaminska, M. Shalak, V.F. Mirande, M. |
| spellingShingle |
Kobbi, L. Dias, J. Octobre, G. Comisso, M. Kaminska, M. Shalak, V.F. Mirande, M. Association of mitochondrial lysyl-tRNA synthetase with HIV-1 GagPol involves catalytic domain of the synthetase and transframe and integrase domains of Pol Вiopolymers and Cell |
| author_facet |
Kobbi, L. Dias, J. Octobre, G. Comisso, M. Kaminska, M. Shalak, V.F. Mirande, M. |
| author_sort |
Kobbi, L. |
| title |
Association of mitochondrial lysyl-tRNA synthetase with HIV-1 GagPol involves catalytic domain of the synthetase and transframe and integrase domains of Pol |
| title_short |
Association of mitochondrial lysyl-tRNA synthetase with HIV-1 GagPol involves catalytic domain of the synthetase and transframe and integrase domains of Pol |
| title_full |
Association of mitochondrial lysyl-tRNA synthetase with HIV-1 GagPol involves catalytic domain of the synthetase and transframe and integrase domains of Pol |
| title_fullStr |
Association of mitochondrial lysyl-tRNA synthetase with HIV-1 GagPol involves catalytic domain of the synthetase and transframe and integrase domains of Pol |
| title_full_unstemmed |
Association of mitochondrial lysyl-tRNA synthetase with HIV-1 GagPol involves catalytic domain of the synthetase and transframe and integrase domains of Pol |
| title_sort |
association of mitochondrial lysyl-trna synthetase with hiv-1 gagpol involves catalytic domain of the synthetase and transframe and integrase domains of pol |
| publisher |
Інститут молекулярної біології і генетики НАН України |
| publishDate |
2011 |
| url |
http://dspace.nbuv.gov.ua/handle/123456789/156406 |
| citation_txt |
Association of mitochondrial lysyl-tRNA synthetase with
HIV-1 GagPol involves catalytic domain of the synthetase
and transframe and integrase domains of Pol
/ L. Kobbi, J. Dias, G. Octobre, M. Comisso, M. Kaminska, V.F. Shalak, M. Mirande
// Вiopolymers and Cell. — 2011. — Т. 27, № 5. — С. 381-382. — Бібліогр.: 8 назв. — англ. |
| series |
Вiopolymers and Cell |
| work_keys_str_mv |
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2025-07-14T06:30:17Z |
| last_indexed |
2025-07-14T06:30:17Z |
| _version_ |
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