Поширення пшенично-житньої транслокації в зразках пшениці м’якої озимої (Triticum aestivum L.) української селекції
Aims. The aim of our research was identification of wheat-rye translocation in soft winter wheat samples of Ukrainian breeding. Methods. DNA was isolated from a mixture of five seeds. Presence of wheat-rye translocation was studied by polymerase chain reaction (PCR). Results. To identify wheat-rye...
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| Date: | 2015 |
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| Main Authors: | , , , |
| Format: | Article |
| Language: | Ukrainian |
| Published: |
Інститут молекулярної біології і генетики НАН України
2015
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| Series: | Фактори експериментальної еволюції організмів |
| Subjects: | |
| Online Access: | http://dspace.nbuv.gov.ua/handle/123456789/177541 |
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| Journal Title: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Cite this: | Поширення пшенично-житньої транслокації в зразках пшениці м’якої озимої (Triticum aestivum L.) української селекції / Г.П. Зайцева, Г.Є. Акініна, О.В. Твердохліб, В.М. Попов // Фактори експериментальної еволюції організмів: Зб. наук. пр. — 2015. — Т. 17. — С. 303-307. — Бібліогр.: 21 назв. — укр. |
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Digital Library of Periodicals of National Academy of Sciences of Ukraine| Summary: | Aims. The aim of our research was identification of wheat-rye translocation in soft winter wheat samples of Ukrainian
breeding. Methods. DNA was isolated from a mixture of five seeds. Presence of wheat-rye translocation was studied by
polymerase chain reaction (PCR). Results. To identify wheat-rye translocations used two types of DNA markers – RIS
and SCM 9. The presence of RIS-marker indicates the presence of rye short chromosome arm 1RS in wheat genome,
SCM9-marker indicates localization of rye translocation in wheat genome – 1BL or 1AL chromosome. Conclusions.
We revealed 1RS rye translocation in following samples of soft winter wheat: line 808/10 and varieties Al’ians, Doridna
(the Plant Production Institute nd. a. V.Ya. Yuriev); Kryzhynka, Favorytka and Spasivka (the Mironivskiy Institute of
wheat nd. a. V.M. Remeslo). The 1RS translocation in 1BL wheat chromosome was identified in varieties Doridna,
Kryzhynka, Favorytka and line 808/10. The presence of rye translocations in 1AL wheat chromosome was revealed in
variety Spasivka. We did not identify rye translocation in wheat samples of Odessa breeding.
Keywords: wheat-rye translocation, DNA markers, T. aestivum L. |
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