Low-temperature ozone sterilizer based on reactor with electrolityc cell
Low-temperature ozone sterilizer based on reactor with electrolytic cell for processing small-sized surgical and dental instruments has been developed. The maximal ozone concentration in water comprised 7.5 mg/l during 10 min of operation. It was revealed that the highest ozone concentration was ach...
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irk-123456789-1947062023-11-29T11:14:21Z Low-temperature ozone sterilizer based on reactor with electrolityc cell Lozina, A.S. Taran, V.S. Krasnyj, V.V. Taran, A.V. Chechelnitskyi, O.G. Gnidenko, Yu.P. Low temperature plasma and plasma technologies Low-temperature ozone sterilizer based on reactor with electrolytic cell for processing small-sized surgical and dental instruments has been developed. The maximal ozone concentration in water comprised 7.5 mg/l during 10 min of operation. It was revealed that the highest ozone concentration was achieved in 400 ml water volume. When increasing the volume to 600 ml, the ozone concentration in water was minimal. The method of neutralizing of alkaline solution from the surface of medical instruments was investigated. Samples for elimination of alkaline solution were processed in 1 % alcohol solution of phenolphthalein. It has been monitored that the time required to remove alkaline solution from the surface of medical instruments is not less than 20 min. The carried out experiments on removal of organic residues (blood) from medical instruments showed that the traces of blood were not presented on the surface after 10-min treatment (the "Delatest" test system was used to determine the number of blood traces). Представлений низькотемпературний озоновий стерилізатор на базі реактора з електролітичним елементом для обробки малогабаритних хірургічних та стоматологічних інструментів. Максимальна концентрація озону у воді становила 7,5 мг/л протягом 10 хв. Проведені дослідження показали, що найбільша концентрація озону у воді в ємності для стерилізації досягається при обсязі води 400 мл. При збільшенні обсягу води до 600 мл концентрація озону у воді має мінімальне значення. Досліджувався метод нейтралізації лужного розчину на поверхні медичного інструменту. Проби на усунення лужного розчину проводилися за допомогою 1 %-го спиртового розчину фенолфталеїну. Встановлено, що для усунення лужного розчину з поверхні інструменту за допомогою озонування необхідно не менше 20 хв. Проведені експерименти з усунення органічних залишків (кров) з медичного інструменту показали, що сліди крові не були представлені на поверхні інструменту після 10-хвилинної обробки (для визначення кількості слідів крові використана тестова система «Делатест»). Представлен низкотемпературный озоновый стерилизатор на основе реактора с электролитической ячейкой для обработки малогабаритных хирургических и стоматологических инструментов. Максимальная концентрация озона в воде составляла 7,5 мг/л в течение 10 мин. Проведенные исследования показали, что наибольшая концентрация озона в воде в емкости для стерилизации достигается при объеме воды 400 мл. При увеличении объема воды до 600 мл концентрация озона в воде имеет минимальное значение. Исследован метод нейтрализации щелочного раствора с поверхности медицинского инструмента. Образцы для удаления щелочного раствора обрабатывали 1 %-ным спиртовым раствором фенолфталеина. Установлено, что для удаления щелочного раствора с поверхности инструмента с помощью озонирования требуется время не менее 20 мин. Проведенные эксперименты по удалению органических остатков (крови) с поверхности медицинского инструмента показали, что следы крови не были обнаружены на поверхности инструмента после 10-мин обработки (для тестирования следов крови использовалась тест-система «Делатест»). 2019 Article Low-temperature ozone sterilizer based on reactor with electrolityc cell / A.S. Lozina, V.S. Taran, V.V. Krasnyj, A.V. Taran, O.G. Chechelnitskyi, Yu.P. Gnidenko // Problems of atomic science and technology. — 2019. — № 1. — С. 149-151. — Бібліогр.: 5 назв. — англ. 1562-6016 PACS: 52.80.Hc, 41.75.Lx, 41.60.Bq http://dspace.nbuv.gov.ua/handle/123456789/194706 en Вопросы атомной науки и техники Національний науковий центр «Харківський фізико-технічний інститут» НАН України |
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Digital Library of Periodicals of National Academy of Sciences of Ukraine |
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Low temperature plasma and plasma technologies Low temperature plasma and plasma technologies |
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Low temperature plasma and plasma technologies Low temperature plasma and plasma technologies Lozina, A.S. Taran, V.S. Krasnyj, V.V. Taran, A.V. Chechelnitskyi, O.G. Gnidenko, Yu.P. Low-temperature ozone sterilizer based on reactor with electrolityc cell Вопросы атомной науки и техники |
| description |
Low-temperature ozone sterilizer based on reactor with electrolytic cell for processing small-sized surgical and dental instruments has been developed. The maximal ozone concentration in water comprised 7.5 mg/l during 10 min of operation. It was revealed that the highest ozone concentration was achieved in 400 ml water volume. When increasing the volume to 600 ml, the ozone concentration in water was minimal. The method of neutralizing of alkaline solution from the surface of medical instruments was investigated. Samples for elimination of alkaline solution were processed in 1 % alcohol solution of phenolphthalein. It has been monitored that the time required to remove alkaline solution from the surface of medical instruments is not less than 20 min. The carried out experiments on removal of organic residues (blood) from medical instruments showed that the traces of blood were not presented on the surface after 10-min treatment (the "Delatest" test system was used to determine the number of blood traces). |
| format |
Article |
| author |
Lozina, A.S. Taran, V.S. Krasnyj, V.V. Taran, A.V. Chechelnitskyi, O.G. Gnidenko, Yu.P. |
| author_facet |
Lozina, A.S. Taran, V.S. Krasnyj, V.V. Taran, A.V. Chechelnitskyi, O.G. Gnidenko, Yu.P. |
| author_sort |
Lozina, A.S. |
| title |
Low-temperature ozone sterilizer based on reactor with electrolityc cell |
| title_short |
Low-temperature ozone sterilizer based on reactor with electrolityc cell |
| title_full |
Low-temperature ozone sterilizer based on reactor with electrolityc cell |
| title_fullStr |
Low-temperature ozone sterilizer based on reactor with electrolityc cell |
| title_full_unstemmed |
Low-temperature ozone sterilizer based on reactor with electrolityc cell |
| title_sort |
low-temperature ozone sterilizer based on reactor with electrolityc cell |
| publisher |
Національний науковий центр «Харківський фізико-технічний інститут» НАН України |
| publishDate |
2019 |
| topic_facet |
Low temperature plasma and plasma technologies |
| url |
http://dspace.nbuv.gov.ua/handle/123456789/194706 |
| citation_txt |
Low-temperature ozone sterilizer based on reactor with electrolityc cell / A.S. Lozina, V.S. Taran, V.V. Krasnyj, A.V. Taran, O.G. Chechelnitskyi, Yu.P. Gnidenko // Problems of atomic science and technology. — 2019. — № 1. — С. 149-151. — Бібліогр.: 5 назв. — англ. |
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Вопросы атомной науки и техники |
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2025-07-16T22:10:21Z |
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2025-07-16T22:10:21Z |
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| fulltext |
ISSN 1562-6016. ВАНТ. 2019. №1(119)
PROBLEMS OF ATOMIC SCIENCE AND TECHNOLOGY. 2019, № 1. Series: Plasma Physics (25), p. 149-151. 149
LOW-TEMPERATURE OZONE STERILIZER BASED ON REACTOR
WITH ELECTROLITYC CELL
A.S. Lozina
1
, V.S. Taran
1
, V.V. Krasnyj
1
, A.V. Taran
1
, O.G. Chechelnitskyi
1
,
Yu.P. Gnidenko
2
1
National Science Center “Kharkov Institute of Physics and Technology”,
Institute of Plasma Physics, Kharkiv, Ukraine;
2
V.N. Karazin Kharkiv National University, Kharkiv, Ukraine
E-mail: vtaran@ ipp.kharkov.ua
Low-temperature ozone sterilizer based on reactor with electrolytic cell for processing small-sized surgical and
dental instruments has been developed. The maximal ozone concentration in water comprised 7.5 mg/l during
10 min of operation. It was revealed that the highest ozone concentration was achieved in 400 ml water volume.
When increasing the volume to 600 ml, the ozone concentration in water was minimal. The method of neutralizing
of alkaline solution from the surface of medical instruments was investigated. Samples for elimination of alkaline
solution were processed in 1 % alcohol solution of phenolphthalein. It has been monitored that the time required to
remove alkaline solution from the surface of medical instruments is not less than 20 min. The carried out
experiments on removal of organic residues (blood) from medical instruments showed that the traces of blood were
not presented on the surface after 10-min treatment (the "Delatest" test system was used to determine the number of
blood traces).
PACS: 52.80.Hc, 41.75.Lx, 41.60.Bq
INTRODUCTION
Ozone has long been recognized as a useful
chemical product, valuable, in particular, for its
outstanding oxidative activity, bactericidal and
antimicrobial properties. Due to this activity, it finds
wide application in the processes of disinfection and
sterilization. In fact, it kills bacteria faster than chlorine
decomposing organic molecules. Ozonation can remove
cyanides, phenols, iron, manganese and detergents and
can destroy various viruses.
With the help of ozone sterilization, an effective
control against pathogenic flora is provided, including
the causative agents of especially dangerous infections:
Pseudomonas Aeruginosa, Staphylococcus, Influenza
virus, Hepatitis B, Tuberculosis, Klebsiella, etc. [1].
Ozone sterilization is quick and effective and in
some cases, the exposure time can be measured in
seconds. If the ozone concentration is insufficient, the
exposure time increases up to 1…2 hours without
affecting the quality of sterilization.
In terms of sterilization quality and technical
characteristics, the ozone sterilizer is superior to
ultraviolet sterilizers and steam autoclaves, liquid and
gas sterilization. The bactericidal ozone effect within 3
to 5 min is analogous to the effect of quartz irradiation
for an hour.
Sterilization with ozone is characterized by low
temperature (room temperature) during the sterilization
cycle, low energy consumption, it does not require
chemicals to be recycled, does not require product
washing or aeration after the sterilization cycle. Ozone
is converted into oxygen, after the end of the
sterilization cycle.
The modern method of obtaining ozone is carried
out by means of a dielectric barrier discharge with the
use of dry air or dry oxygen as initial gas. Ozone
produced by these generators is perfectly suited for
wastewater treatment, drinking water purification, etc.,
but has limitations when working with disinfection and
sterilization systems [2-5].
The innovative and efficient way to produce ozone
presented in this paper is to use an electrolytic ozone
generator that divides water into basic elements and
then converts some of the released oxygen into ozone.
1. EXPERIMENTAL SETUP
Ozone sterilizer with a capacity of 1 l is designed for
low-temperature ozone sterilization of medical
instruments (surgical, dental, plastic products, silicone,
and glass). The sterilization vessel is made of stainless
steel. The chamber is sealed with a lid (10 mm thick
acrylic glass), which has an ozone resistant isolation.
The general view of the device is shown in Fig. 1.
Fig. 1. General view of the sterilizer with electrolytic
cell
As an ozone generator, an electrolytic ozone
generator type as PEM-800 is used. This electrolytic
system is a fundamentally different method of water
dissociation because a solid polymer membrane is used
as an electrolyte instead of a liquid – this feature,
http://medicine.karazin.ua/
150 ISSN 1562-6016. ВАНТ. 2019. №1(119)
combined with an appropriate controlled intermediate
anode reaction, makes it particularly suitable for
disinfection and sterilization of water. Ozone dissolves
in water as soon as it forms – this leads to sterilization
with a minimum amount of equipment.
The membrane, which functions as an electrolyte
and a separator between the anode and the cathode,
contacts on both sides with activated porous electrodes.
As a result of the action of direct current, the water
supplied to the anode side of the cell dissociates at the
interface between the anode and the membrane [3].
Fig. 2. Block diagram of measurement of ozone
concentration in the sterilizer: 1 – sterilization
chamber; 2 – water pump for circulation through the
monochromator system; 3 – PMT; 4 – MDR-2;
5 – optical system with two quartz cells; 6 – UV source
mercury lamp; 7 – PC
The compressor, which is part of the sterilizer, is
necessary to neutralize ozone in the sterilization vessel
after the sterilization process is completed. Excess
portions of ozone pass into the destructor, where they
decompose to oxygen and exit into the atmosphere. The
concentration of ozone in the sterilization vessel was
determined on an automated stand using the MDR-2
monochromator (LOMO, Russia) and the photoelectric
multiplier (PMT). The scheme for measuring the ozone
concentration in the sterilizer is shown in Fig. 2.
2. RESULTS AND DISCUSSION
The experiments carried out have shown that the
highest ozone concentration in water in the sterilization
bath was achieved in a water volume of 400 ml. When
increasing the volume to 600 ml, the ozone
concentration in water had a minimum value (Fig. 3).
For 300 ml water volume, the ozone concentration at the
initial moment increases faster, and then the ozone
concentration rate tends to decrease. We speculate that
this is due to the heating of water in the sterilization
volume, which impairs the ozone solubility. The
dependence of the water heating temperature on the
water volume is shown in Fig. 4.
The rate of ozone decomposition in water was
estimated using the half-life term, i.e. the time during
which the ozone concentration is halved. After reaching
the operating mode, in which the ozone concentration
reached a maximum value and was constant, the power
of the electrolytic cell was turned off (Fig. 5).
The method of neutralizing an alkaline solution on
the surface of medical instruments was investigated [4].
Samples for elimination of alkaline solution were
carried out with a 1 % alcohol solution of
phenolphthalein.
Fig. 3. Dependence of ozone concentration in water on
time at different water volumes
Fig. 4. Dependence of the water temperature in the
sterilization bath on the volume
Fig. 5. Growth rate and ozone half-life
In our case, a change in color of the solution
indicates the quality of the treatment. It has been
established that the time required for removal of
alkaline solution from the surface of the instrument is
not less than 20 min.
Fig. 6 shows the visual change in color of the
alkaline solution during ozonation.
ISSN 1562-6016. ВАНТ. 2019. №1(119) 151
Fig. 6. Change in color of the alkaline solution during
sterilization
The experiments have been carried out to eliminate
organic residues (blood) from the surface of a medical
instrument showed that the blood traces were not
presented on the surface after 10-min treatment (the
"Delatest" test system was used to determine the
number of blood traces).
CONCLUSIONS
1. Low-temperature ozone sterilizer based on reactor
with electrolytic cell for processing of small-sized
surgical and dental instruments has been developed.
Studies have shown that the highest ozone concentration
in water in the sterilization bath was achieved at 400 ml.
When increasing the volume of water to 600 ml, the
ozone concentration is minimal.
2. The method of neutralizing of alkaline solution
from the surface of a medical instrument was
investigated. Samples for elimination of an alkaline
solution were processed in 1% alcohol solution of
phenolphthalein. It has been established that than the
time required for removal of an alkaline solution from
the surface of the instrument by means of ozonization, is
not less than 20 min. The carried out experiments to
eliminate organic residues (blood) from a medical
instrument showed that the traces of blood were not
presented on the surface of the instrument after a 10-
min treatment (the "Delatest" test system was used to
determine the number of blood traces).
REFERENCES
1. Vince Ciufia. Ozone sanitization of ultrapure water
systems innovations in dissolved ozone measurement by
UV photometry // IOA IUVA, World Conference, Vegas,
NV, September, 23-28, 2013.
2. V.V. Krasnyj, A.V. Klosovskij, T.A. Panasko,
O.M. Shvets, O.T. Semenova,V.S. Taran, V.I. Tereshin.
Plasma sterilizer with ultrasonic cavitation // Problems
of Atomic Science and Technology. Series “Plasma
Physics”. 2007, № 1, p.188-190.
3. Sang-Do Han, Jung-Duk Kim, Kwang-Shik Myung,
et al. Electro-chemical production of ozone using water
electrolysis cell of solid polymer electrolyte (SPE) //
Indian Journal of Chemical Technology. 2006, v. 13,
p. 156-161.
4. V.S. Taran, V.V. Krasnyj, A.V. Schebetun,
A.S. Lozina. Devices for pre-sterilization treatment of
endoscopes by ozone and ultrasound // Problems of
Atomic Science and Technology. Series “Plasma
Physics”. 2014, № 6, p. 230-232.
5. V.S. Taran, I.E. Garkusha, et al. Recent developments
of plasma-based technologies for medicine and industry
// Nukleonika. 2012, v. 57(2), p. 277-282 (in Russian).
Article received 18.11.2018
[
НИЗКОТЕМПЕРАТУРНЫЙ ОЗОНОВЫЙ СТЕРИЛИЗАТОР С РЕАКТОРОМ НА ОСНОВЕ
ЭЛЕКТРОЛИТИЧЕСКОЙ ЯЧЕЙКИ
[
А.С. Лозина, В.С. Таран, В.В. Красный, А.В. Таран, О.Г. Чечельницкий, Ю.П. Гниденко
[
Представлен низкотемпературный озоновый стерилизатор на основе реактора с электролитической
ячейкой для обработки малогабаритных хирургических и стоматологических инструментов. Максимальная
концентрация озона в воде составляла 7,5 мг/л в течение 10 мин. Проведенные исследования показали, что
наибольшая концентрация озона в воде в емкости для стерилизации достигается при объеме воды 400 мл.
При увеличении объема воды до 600 мл концентрация озона в воде имеет минимальное значение.
Исследован метод нейтрализации щелочного раствора с поверхности медицинского инструмента. Образцы
для удаления щелочного раствора обрабатывали 1 %-ным спиртовым раствором фенолфталеина.
Установлено, что для удаления щелочного раствора с поверхности инструмента с помощью озонирования
требуется время не менее 20 мин. Проведенные эксперименты по удалению органических остатков (крови) с
поверхности медицинского инструмента показали, что следы крови не были обнаружены на поверхности
инструмента после 10-мин обработки (для тестирования следов крови использовалась тест-система
«Делатест»).
НИЗЬКОТЕМПЕРАТУРНИЙ ОЗОНОВИЙ СТЕРИЛІЗАТОР З РЕАКТОРОМ НА ОСНОВІ
ЕЛЕКТРОЛІТИЧНОГО ЕЛЕМЕНТУ
[
А.С. Лозіна, В.С. Таран, В.В. Красний, А.В. Таран, О.Г. Чечельницький, Ю.П. Гніденко
[
Представлений низькотемпературний озоновий стерилізатор на базі реактора з електролітичним
елементом для обробки малогабаритних хірургічних та стоматологічних інструментів. Максимальна
концентрація озону у воді становила 7,5 мг/л протягом 10 хв. Проведені дослідження показали, що
найбільша концентрація озону у воді в ємності для стерилізації досягається при обсязі води 400 мл. При
збільшенні обсягу води до 600 мл концентрація озону у воді має мінімальне значення. Досліджувався метод
нейтралізації лужного розчину на поверхні медичного інструменту. Проби на усунення лужного розчину
проводилися за допомогою 1 %-го спиртового розчину фенолфталеїну. Встановлено, що для усунення
лужного розчину з поверхні інструменту за допомогою озонування необхідно не менше 20 хв. Проведені
експерименти з усунення органічних залишків (кров) з медичного інструменту показали, що сліди крові не
були представлені на поверхні інструменту після 10-хвилинної обробки (для визначення кількості слідів
крові використана тестова система «Делатест»).
|