Suture material on the base of polyglycoidsbiogenic stimulator for formation of microcirculatory channels/cycle
Экспериментально обосновано применение шовных материалов на основе полигликолидов в качестве биогенного стимулятора формирования микроциркуляторного русла. Это свойство полигликолидов может найти применение в клинической практике для оптимизации микроциркуляторного русла у больных с хроническими об...
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irk-123456789-254822013-03-18T03:08:50Z Suture material on the base of polyglycoidsbiogenic stimulator for formation of microcirculatory channels/cycle Hryvenko, S.H. Hryvenko, I.S. Оригинальные статьи Экспериментально обосновано применение шовных материалов на основе полигликолидов в качестве биогенного стимулятора формирования микроциркуляторного русла. Это свойство полигликолидов может найти применение в клинической практике для оптимизации микроциркуляторного русла у больных с хроническими облитерирующими заболеваниями нижних конечностей. Експериментально обґрунтоване застосування шовних матеріалів на основі полигликолидов як біогенний стимулятор формування микроциркуляторного русла. Це властивість полигликолидов може знайти застосування в клінічній практиці для оптимізації микроциркуляторного русла у хворих із хронічними облитерирующими захворюваннями нижніх кінцівок. 2010 Article Suture material on the base of polyglycoidsbiogenic stimulator for formation of microcirculatory channels/cycle / S.H. Hryvenko, I.S. Hryvenko // Таврический медико-биологический вестник. — 2010. — Т. 13, № 1 (49). — С. 40-43. — Бібліогр.: 14 назв. — англ. 2070-8092 http://dspace.nbuv.gov.ua/handle/123456789/25482 617-089:616.13-008.64-(59.082):616-092.4 en Таврический медико-биологический вестник Кримський науковий центр НАН України і МОН України |
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Оригинальные статьи Оригинальные статьи Hryvenko, S.H. Hryvenko, I.S. Suture material on the base of polyglycoidsbiogenic stimulator for formation of microcirculatory channels/cycle Таврический медико-биологический вестник |
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Экспериментально обосновано применение шовных материалов на основе полигликолидов в качестве биогенного стимулятора формирования микроциркуляторного русла. Это свойство полигликолидов может найти применение в клинической практике для оптимизации микроциркуляторного русла у больных с хроническими облитерирующими заболеваниями нижних
конечностей. |
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Hryvenko, S.H. Hryvenko, I.S. |
| author_facet |
Hryvenko, S.H. Hryvenko, I.S. |
| author_sort |
Hryvenko, S.H. |
| title |
Suture material on the base of polyglycoidsbiogenic stimulator for formation of microcirculatory channels/cycle |
| title_short |
Suture material on the base of polyglycoidsbiogenic stimulator for formation of microcirculatory channels/cycle |
| title_full |
Suture material on the base of polyglycoidsbiogenic stimulator for formation of microcirculatory channels/cycle |
| title_fullStr |
Suture material on the base of polyglycoidsbiogenic stimulator for formation of microcirculatory channels/cycle |
| title_full_unstemmed |
Suture material on the base of polyglycoidsbiogenic stimulator for formation of microcirculatory channels/cycle |
| title_sort |
suture material on the base of polyglycoidsbiogenic stimulator for formation of microcirculatory channels/cycle |
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Кримський науковий центр НАН України і МОН України |
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2010 |
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Оригинальные статьи |
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http://dspace.nbuv.gov.ua/handle/123456789/25482 |
| citation_txt |
Suture material on the base of polyglycoidsbiogenic stimulator for formation of microcirculatory channels/cycle / S.H. Hryvenko, I.S. Hryvenko // Таврический медико-биологический вестник. — 2010. — Т. 13, № 1 (49). — С. 40-43. — Бібліогр.: 14 назв. — англ. |
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Таврический медико-биологический вестник |
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AT hryvenkosh suturematerialonthebaseofpolyglycoidsbiogenicstimulatorforformationofmicrocirculatorychannelscycle AT hryvenkois suturematerialonthebaseofpolyglycoidsbiogenicstimulatorforformationofmicrocirculatorychannelscycle |
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2025-07-03T05:00:40Z |
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40
ТАВРИЧЕСКИЙ МЕДИКО-БИОЛОГИЧЕСКИЙ ВЕСТНИК2010, том 13, № 1 (49)
УДК 617-089:616.13-008.64-(59.082):616-092.4
© S. H. Hryvenko, I. S. Hryvenko, 2010.
SUTURE MATERIAL ON THE BASE OF POLYGLYCOIDS-
BIOGENIC STIMULATOR FOR FORMATION OF
MICROCIRCULATORY CHANNELS/CYCLE
S. H. Hryvenko, I. S. Hryvenko
Crimean State Medical University named after S.I.Georgievsky, Ukraine
ШОВНІ МАТЕРІАЛИ НА ОСНОВІ ПОЛИГЛИКОЛИДОВ - БІОГЕННІ СТИМУЛЯТОРИ ФОРМУВАННЯ
МИКРОЦИРКУЛЯТОРНОГО РУСЛА
С.Г. Гривенко, И.С. Гривенко
РЕЗЮМЕ
Експериментально обґрунтоване застосування шовних матеріалів на основі полигликолидов
як біогенний стимулятор формування микроциркуляторного русла. Це властивість полигликолидов
може знайти застосування в клінічній практиці для оптимізації микроциркуляторного русла у хворих із
хронічними облитерирующими захворюваннями нижніх кінцівок.
ШОВНЫЕ МАТЕРИАЛЫ НА ОСНОВЕ ПОЛИГЛИКОЛИДОВ – БИОГЕННЫЕ СТИМУЛЯТОРЫ
ФОРМИРОВАНИЯ МИКРОЦИРКУЛЯТОРНОГО РУСЛА
С.Г. Гривенко, И.С. Гривенко
РЕЗЮМЕ
Экспериментально обосновано применение шовных материалов на основе полигликолидов в
качестве биогенного стимулятора формирования микроциркуляторного русла. Это свойство
полигликолидов может найти применение в клинической практике для оптимизации
микроциркуляторного русла у больных с хроническими облитерирующими заболеваниями нижних
конечностей.
Keywords: рolyglycoids, implantation, microcirculatory cycle.
Treatment of chronic arterial insufficiency/chronic
limb ischemia of lower limbs is one of the most grave/
acute problems in surgery. Although during the last
years, thanks to the developments in reconstructive
vascular surgery, immense success has been achieved
in restoration of blood circulation in lower limbs, active
surgical manipulations has its restrictions and algorithm
of conservative therapy practiced at present is only
symptomatic.
This factor does not affectively influence the
pathological basis of chronic arterial insufficiency/
chronic limb ischemia. Basic method of treatment in
patients with occlusive diseases of arteries of lower limbs
in most cases is intravenous infusion of medicaments.
But use of modern medicinal therapy scheme does not
gives enough positive influence on long term results of
treatment and does not decrease numbers of
amputations[11].
It is hereby of great importance to develop new
methods and schemes of chronic arterial insufficiency/
chronic limb ischemia therapy, which will allow us to
neutralize factors of atherogenesis, slow down the
development of sclerotic damage and improve the state
of collateral blood circulation in limbs. In context of the
problem of chronic arterial insufficiency/chronic limb
ischemia, collateral blood circulation represent itself as
the main question and without its solution it is not
possible to choose the rational treatment scheme.
Subsequently many methods to improve collateral blood
circulation has been proposed: osteotomy by Г.А.Или-
зарову-Ф.Н.Зусмановичу [5,6], modification/variants
of revascularative osteotrepanation[2,3,7], treatment
with extravasations[4], treatment under influence of high
intensity laser rays[1].
Experimental research with use of gene engineering
technologies in treatment chronic arterial insufficiency/
chronic limb ischemia has been conducted[8]. The first
clinical results of application of gene which codes for
the growth factor of endothelia for simulation of the
process neoangiogenesis and ischemic limbs has been
received[10,12-15].
To develop and experimentally prove a new method
of microcirculation cycle/channel formation. As the
prototype the method of treatment with extravasations has
been chosen[4]. Authors named multiple aggregations of
formed elements of blood which are left in tissue after
41
О Р И Г И Н А Л Ь Н Ы Е С Т А Т Ь И
infiltration by distal segments of patient’s own blood as
extravasations. In zones of extravasations,
corresponding reaction was observed on the part of
interstitial tissue with formation of excessive areolar (or
loose) connective tissue rich in vessels like minute
arteries, arterioles and capillaries. Improvement in
peripheral blood circulation between procedures of
formation of extravasations is explained by the aseptic
inflammation in tissues of the processed limb.
Within 1-1.5 months or more after formation of
extravasations, Improvement of microcirculation is
caused by inclusion in blood circulation perivasal
vascular system and functioning collaterals with help
of micro vessels of the formed areolar/loose
nonformalized connecting tissue. Negative aspect of this
method is the insufficient clinical affectivity, which is
connected with its excessive invasive process which in
turn is explained by the necessity of getting autologous
blood and its subsequent interstitial introduction.
The designated methodical approach was a
precondition of frequent purulent complications. Besides,
it is necessary to consider the low level of technology,
for reception and subsequent introduction of autologous
blood.
The method proposed by us for formation of
microcirculatory channels/cycles [9], which is based on
which is based on intramuscular implantation of
polymers of glycolic acids. Polyglycolic acid is widely
used in surgery, in form of suture material (dexon, maxon,
biosorb, PGA Resorba, serafit, biosyn). In human
organism, sutures with these material gets hydrolytically
absorbed, i.e. under the influence of water with mild
irritation of tissues by glycolic acid, which then takes
participation in metabolism, decaying on carbonic acid
and water. In case of intramuscular implantation
absorption of this particular type of suture material is
minimum 40 days and full absorption occurs in 60-90
day.
MATERIAL AND METHODS
The experiment was carried out on 75 white rats,
who were administered Nеmbutal/ Aethaminalum-
natrium as anesthesia, with the dose of 0.5 ml of 5% sol
of Aethaminalum-natrium per 1 kg mass of rats. Then in
one of the limbs ligature was implanted in strips (1-3
grams of suture material in 1 sq dm of tissue. Animals
were deduced from experience on 5th, 7th, 14th, 30th
and 90th day, by intra pleural introduction of 1 ml of 5 %
sol of Aethaminalum-natrium. Estimation of efficiency
of process of formation of microcirculatory cycle/
channels was carried out by the means of the histological
analysis of muscles; with preliminary implanted biogenic
stimulator i.e. suture material.
RESULTS AND ANALYSIS
After 5th day post implantation, in the muscular
tissues neogenic capillaries were revealed and from the
7th day intense growth granulation tissue with a
significant amount of vessels was observed. In
histological research of slide stained with hemotoxilin –
eosin on 14th day a region of absorption of suture material
was revealed. On edge of the implanted suture material
elements of «areolar» connective tissue were found. This
connective tissue is represented by collagen fibers,
fibroblasts, fibrocytes and units lymphocytes. Among
areolar/loose connective tissue, hemo-capillaries with
isolated erythrocytes were determined (pic. 1).
Pic 1: Areolar/loose connective tissue, formation of capillaries. Absorption of suture
material. Stained with hemotoxilin – eosin.
From this period, growth of young granulation tissue
with well defined vascular component was revealed and
from 30th day vessels thick walls were revealed (pic. 2).In
the period between 30th and 90th day significant change
in the development of the micro vessels was not seen,
which points to the fact that reaction of microcirculatory
apparatus on heterogenic stimulator ends during the
period of 1 month. On the basis of experiments,
technically based recommendation has been given for
the manufacture of atraumatic straight needle with suture
42
ТАВРИЧЕСКИЙ МЕДИКО-БИОЛОГИЧЕСКИЙ ВЕСТНИК2010, том 13, № 1 (49)
material for transmuscular implantation. This has been
done as none of the suture material manufactures does
not manufacture atraumatic needle-suture complex
which meets these parameters and aims.
Pic 2: Coarse-fibred connecting tissue around suture material. Formed vessels- arterioles.
Stained with hemotoxilin – eosin.
Straight/linear needle polyglycoid suture material
is meant for implantation in muscle of calf as in case of
chronic arterial insufficiency of lower limbs mostly the
distal segments of the limbs are affected. Considering,
that the average diameter of calf of a person ranges
from 10-16 cm, and that the needle should be fixed on
the needle holder, the length of a needle should be not
less than 12-18 cm. It should have certain rigidity so
that not to get deformed while movement through tissue
of the calf ( skin hypodermic layer, fascia, muscles ).
The needle is used only as a conductor for implantation
of the suture material, and thus trauma should be
minimal. The most optimal in this case is the choice of
piercing cutting edge needle as well as the suture as
well as the choice of suture material-polyglycoids due
to its ability of bio-destruction with minimal inflammatory
reaction. Therefore the suture material should be small
diameter so that its absorption occurs quickly enough.
In our opinion the parameters for these purposes are
satisfied by a suture material with the size 2-0 or 3-0.
In pic 3,4 Transverse cross section calf in different
levels is represented. Various variants of transmuscular
implantation of polyglycoids with help of atraumatic
needle-suture complex is demonstrated.
Pic 3: Transverse Cross section through middle
part of calf. Black dotted line shows the
direction of transmuscular implantation of
polyglycoids.
Pic 4: Transverse Cross section through lower one
third part of calf. Black dotted line shows the
direction of transmuscular implantation of
polyglycoids.
43
О Р И Г И Н А Л Ь Н Ы Е С Т А Т Ь И
CONCLUSIONS
The proposed procedure provides better possibility
with the prototype level of technology and biogenic
efficiency and can be used in clinical practice or
optimization of microcirculatory system of tissue of
lower limb.
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