Water deficit induces changes in H⁺-ATPase activity and its gene expres sion in Zea mays L. roots
We investigated the functioning and the gene expression of H⁺-ATPase in the microsomal fraction enri ched with plasmalemma vesicles isolated from roots of two Zea mays cultivars: Dostatok (drought resistant) and Pereyaslivskiy (moderately tolerant to drought). Both cultivars grew during 21 days un...
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| Date: | 2016 |
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| Main Authors: | , |
| Format: | Article |
| Language: | English |
| Published: |
Видавничий дім "Академперіодика" НАН України
2016
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| Series: | Доповіді НАН України |
| Subjects: | |
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| Journal Title: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Cite this: | Water deficit induces changes in H⁺-ATPase activity and its gene expres sion in Zea mays L. roots / I.I. Ovrutska, D.A. Bluma // Доповіді Національної академії наук України. — 2016. — № 10. — С. 84-87. — Бібліогр.: 8 назв. — англ. |
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Digital Library of Periodicals of National Academy of Sciences of Ukraine| Summary: | We investigated the functioning and the gene expression of H⁺-ATPase in the microsomal fraction enri ched
with plasmalemma vesicles isolated from roots of two Zea mays cultivars: Dostatok (drought resistant)
and Pereyaslivskiy (moderately tolerant to drought). Both cultivars grew during 21 days under the optimal
water supply (soil moisture — 70 %, control) and during 10 days under the water deficit conditions (soil
moisture — 30 %, experiment). An increase in the H⁺-ATPase hydrolytic activity in both cultivars under
the water stress has been detected under drought. The enzyme hydrolytic activity is increased by twice in
Dostatok and by1.3 times in Pereyaslivskiy. The H⁺-ATPase gene expression levels in both cultivars also
increased under water deficit. It is shown that, in roots of both maize cultivars, the increase of four isoforms
of H⁺-ATPase gene expression correlates with the increase of the hydrolytic enzyme activity under
water stress conditions. However, these parameters are more sustainable in the drought-resistant cultivar
Dosta tok, by allowing one value to use the hydrolytic activity of H⁺-ATPase as a biomarker of drought resistance
for corn varieties. |
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