TNFα receptor1 drives hypoxia-promoted invasiveness of human melanoma cells
Aim: Oxygen deprivation leading to hypoxia represents a common feature of advanced solid tumors, able to control several aspects of tumor progression. Indeed, ability to respond to changes in oxygen partial pressure represents a hallmark of malignant cells. Aim of this study is to disclose new pathw...
Збережено в:
| Дата: | 2013 |
|---|---|
| Автори: | , , , |
| Формат: | Стаття |
| Мова: | English |
| Опубліковано: |
Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
2013
|
| Назва видання: | Experimental Oncology |
| Теми: | |
| Теги: |
Додати тег
Немає тегів, Будьте першим, хто поставить тег для цього запису!
|
| Назва журналу: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Цитувати: | TNFα receptor1 drives hypoxia-promoted invasiveness of human melanoma cells / C. Marconi, S. Peppicelli, F. Bianchini, L. Calorini // Experimental Oncology. — 2013. — Т. 35, № 3. — С. 187-191. — Бібліогр.: 24 назв. — англ. |
Репозитарії
Digital Library of Periodicals of National Academy of Sciences of Ukraine| id |
oai:nasplib.isofts.kiev.ua:123456789-145228 |
|---|---|
| record_format |
dspace |
| spelling |
oai:nasplib.isofts.kiev.ua:123456789-1452282025-02-23T17:42:45Z TNFα receptor1 drives hypoxia-promoted invasiveness of human melanoma cells Marconi C. Peppicelli S. Bianchini F. Calorini L. Original contributions Aim: Oxygen deprivation leading to hypoxia represents a common feature of advanced solid tumors, able to control several aspects of tumor progression. Indeed, ability to respond to changes in oxygen partial pressure represents a hallmark of malignant cells. Aim of this study is to disclose new pathway of hypoxia-induced tumor cell invasion. Materials and Methods: Hs294T human melanoma cells were grown in a gas mixture containing 0.3 % , and used to evaluate invasion on Matrigel-coated polycarbonate filters mounted in Boyden's chambers, MMP release and expression of inflammatory receptors and their ligands. Results: We demonstrate that hypoxia promotes the expression of TNFa receptor 1 (TNFαR1) able to drive a higher ability to penetrate Matrigel-coated filters of Hs294T human melanoma cells, an effect does not mediated by hypoxia-inducible factor (HIF)-1α. Conclusion: Expression inflammatory cytokine receptors in hypoxic human melanoma cells might provide a new target for improving strategies against local and distant tumor cell diffusion. Key Words: TNFa receptor 1, Hs294T human melanoma cells, hypoxia, cell invasiveness, metalloproteinase 2 and 9. This work was supported by grants from Ente Cassa di Risparmio di Firenze (ECRF) and Instituto Toscano Tumori (ITT). 2013 Article TNFα receptor1 drives hypoxia-promoted invasiveness of human melanoma cells / C. Marconi, S. Peppicelli, F. Bianchini, L. Calorini // Experimental Oncology. — 2013. — Т. 35, № 3. — С. 187-191. — Бібліогр.: 24 назв. — англ. 1812-9269 https://nasplib.isofts.kiev.ua/handle/123456789/145228 en Experimental Oncology application/pdf Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
| institution |
Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| collection |
DSpace DC |
| language |
English |
| topic |
Original contributions Original contributions |
| spellingShingle |
Original contributions Original contributions Marconi C. Peppicelli S. Bianchini F. Calorini L. TNFα receptor1 drives hypoxia-promoted invasiveness of human melanoma cells Experimental Oncology |
| description |
Aim: Oxygen deprivation leading to hypoxia represents a common feature of advanced solid tumors, able to control several aspects of tumor progression. Indeed, ability to respond to changes in oxygen partial pressure represents a hallmark of malignant cells. Aim of this study is to disclose new pathway of hypoxia-induced tumor cell invasion. Materials and Methods: Hs294T human melanoma cells were grown in a gas mixture containing 0.3 % , and used to evaluate invasion on Matrigel-coated polycarbonate filters mounted in Boyden's chambers, MMP release and expression of inflammatory receptors and their ligands. Results: We demonstrate that hypoxia promotes the expression of TNFa receptor 1 (TNFαR1) able to drive a higher ability to penetrate Matrigel-coated filters of Hs294T human melanoma cells, an effect does not mediated by hypoxia-inducible factor (HIF)-1α. Conclusion: Expression inflammatory cytokine receptors in hypoxic human melanoma cells might provide a new target for improving strategies against local and distant tumor cell diffusion. Key Words: TNFa receptor 1, Hs294T human melanoma cells, hypoxia, cell invasiveness, metalloproteinase 2 and 9. |
| format |
Article |
| author |
Marconi C. Peppicelli S. Bianchini F. Calorini L. |
| author_facet |
Marconi C. Peppicelli S. Bianchini F. Calorini L. |
| author_sort |
Marconi C. |
| title |
TNFα receptor1 drives hypoxia-promoted invasiveness of human melanoma cells |
| title_short |
TNFα receptor1 drives hypoxia-promoted invasiveness of human melanoma cells |
| title_full |
TNFα receptor1 drives hypoxia-promoted invasiveness of human melanoma cells |
| title_fullStr |
TNFα receptor1 drives hypoxia-promoted invasiveness of human melanoma cells |
| title_full_unstemmed |
TNFα receptor1 drives hypoxia-promoted invasiveness of human melanoma cells |
| title_sort |
tnfα receptor1 drives hypoxia-promoted invasiveness of human melanoma cells |
| publisher |
Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
| publishDate |
2013 |
| topic_facet |
Original contributions |
| citation_txt |
TNFα receptor1 drives hypoxia-promoted invasiveness of human melanoma cells / C. Marconi, S. Peppicelli, F. Bianchini, L. Calorini // Experimental Oncology. — 2013. — Т. 35, № 3. — С. 187-191. — Бібліогр.: 24 назв. — англ. |
| series |
Experimental Oncology |
| work_keys_str_mv |
AT marconic tnfareceptor1driveshypoxiapromotedinvasivenessofhumanmelanomacells AT peppicellis tnfareceptor1driveshypoxiapromotedinvasivenessofhumanmelanomacells AT bianchinif tnfareceptor1driveshypoxiapromotedinvasivenessofhumanmelanomacells AT calorinil tnfareceptor1driveshypoxiapromotedinvasivenessofhumanmelanomacells |
| first_indexed |
2025-07-22T04:24:13Z |
| last_indexed |
2025-07-22T04:24:13Z |
| _version_ |
1838319669738471424 |