Spreading and proliferation of cultured rat bone marrow stromal cells on the surface of bioactive glass ceramics
Aim. To study spreading profile, cytoskeleton organization and proliferation of bone marrow stromal cells upon cultivation on the glass-crystalline material (GCM) surfaces with different chemical composition and solubility. Methods. GCMs with different CaO: P₂O₅ ratios were used. Actin cytoskeleton...
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| Date: | 2017 |
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| Main Authors: | , , , , |
| Format: | Article |
| Language: | English |
| Published: |
Інститут молекулярної біології і генетики НАН України
2017
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| Series: | Вiopolymers and Cell |
| Subjects: | |
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| Journal Title: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Cite this: | Spreading and proliferation of cultured rat bone marrow stromal cells on the surface of bioactive glass ceramics / V.V. Kiroshka, O.V. Savvova, Yu.O. Bozhkova, I.V. Tamarina, A.I. Fesenko // Вiopolymers and Cell. — 2017. — Т. 33, № 1. — С. 48-57. — Бібліогр.: 35 назв. — англ. |
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Digital Library of Periodicals of National Academy of Sciences of Ukraine| Summary: | Aim. To study spreading profile, cytoskeleton organization and proliferation of bone marrow stromal cells upon cultivation on the glass-crystalline material (GCM) surfaces with different chemical composition and solubility. Methods. GCMs with different CaO: P₂O₅ ratios were used. Actin cytoskeleton in cells was visualized using fluorescent TRITC-conjugated phalloidin. Cell proliferation was studied using MTT test. Results. Cell cultivation on highly soluble B-series GCM (Ca/P=5) led to appearance of fibroblast-like cells; their actin cytoskeleton filaments were uniformly distributed within the cytoplasm. In this case, proliferation dynamics was similar to that under cultivation on plastic. Cultivation on A and C series of GCM with a reduced Ca/P (1.4–2.58) resulted in a decrease of cell spreading and their proliferation index (up to 2–3 times) relative to the control. Conclusions. The material solubility and Ca/P superficial ratio are the main factors, determining cell interaction with GCMs. |
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