Development and characterization of porous functionalized collagen scaffolds for delivery of FGF-2
Aim. To develop the porous functionalized collagen scaffold for the delivery of FGF-2 and studying its properties in vitro and in vivo. Methods. Porous collagen scaffolds were prepared by freeze- drying collagen I solutions containing the polymer developed on the basis of cross-linked modified hepar...
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| Datum: | 2014 |
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| Hauptverfasser: | , , , , , , , , |
| Format: | Artikel |
| Sprache: | English |
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Інститут молекулярної біології і генетики НАН України
2014
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| Schriftenreihe: | Вiopolymers and Cell |
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| Назва журналу: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Zitieren: | Development and characterization of porous functionalized collagen scaffolds for delivery of FGF-2 / Ia.O. Pokholenko, M.D. Chetyrkina, L.V. Dubey, I.Ya. Dubey, O.V. Moshynets, E.V. Sheludko, S.P. Shpylova, M.I. Degtiarova, V.A. Kordium // Вiopolymers and Cell. — 2014. — Т. 30, № 3. — С. 216-222. — Бібліогр.: 15 назв. — англ. |
Institution
Digital Library of Periodicals of National Academy of Sciences of Ukraine| Zusammenfassung: | Aim. To develop the porous functionalized collagen scaffold for the delivery of FGF-2 and studying its properties in vitro and in vivo. Methods. Porous collagen scaffolds were prepared by freeze- drying collagen I solutions containing the polymer developed on the basis of cross-linked modified heparin. The scaffolds have been analyzed by SEM, AFM and SCLM. The angiogenic activity of these scaffolds loaded with FGF-2 was tested in a CAM assay. Results. The data obtained by SEM and SCLM analysis revealed that the scaffold mainly has a layered structure with pores forming a connection between the layers. The average pore size of the scaffolds varied from 76 to 150 µm. Scaffolds containing the polymer were able to incorporate human FGF-2. Proposed compositions promoted angiogenesis in CAM assay. Conclusions. The developed porous functionalized collagen scaffold incorporating FGF-2 can be used as a vehicle for the sustained delivery of the growth factor both in vitro and in vivo. |
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