Alternatively spliced short and long isoforms of adaptor protein intersectin 1 form complexes in mammalian cells
Intersectin 1 (ITSN1) is an adaptor protein involved in membrane trafficking and cell signaling. Long and short isoforms of ITSN1 (ITSN1-L and ITSN1-S) are produced by alternative splicing. The aim of our study was to investigate whether ITSN1-L and ITSN1-S could interact in mammalian cells. Metho...
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| Дата: | 2012 |
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| Автори: | , , , , |
| Формат: | Стаття |
| Мова: | English |
| Опубліковано: |
Інститут молекулярної біології і генетики НАН України
2012
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| Назва видання: | Вiopolymers and Cell |
| Теми: | |
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| Назва журналу: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Цитувати: | Alternatively spliced short and long isoforms of adaptor protein intersectin 1 form complexes in mammalian cells / O.S. Gubar, S.V. Kropyvko, L.O. Tsyba, S. Gasman, A.V. Rynditch // Вiopolymers and Cell. — 2012. — Т. 28, № 6. — С. 429-423. — Бібліогр.: 29 назв. — англ. |
Репозитарії
Digital Library of Periodicals of National Academy of Sciences of Ukraine| Резюме: | Intersectin 1 (ITSN1) is an adaptor protein involved in membrane trafficking and cell signaling. Long and short
isoforms of ITSN1 (ITSN1-L and ITSN1-S) are produced by alternative splicing. The aim of our study was to
investigate whether ITSN1-L and ITSN1-S could interact in mammalian cells. Methods. During this study we
employed immunoprecipitation and confocal microscopy. Results. We have shown that endogenous ITSN1-S coprecipitates with overexpressed ITSN1-L in PC12, 293 and 293T cells. Long and short isoforms of ITSN1 also colocalize in 293T cells. Conclusions. ITSN1-L and ITSN1-S form complexes in mammalian cells.
Keywords: ITSN1, alternatively spliced isoforms, adaptor/scaffold proteins. |
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