Generation of monoclonal antibodies specific to ribosomal protein S6 kinase 1
The aim of this study was to produce monoclonal antibodies directed to the N-terminal regulatory region of S6K1, which shows very low homology to S6K2. Methods. Hybridoma technology, ELISA, Western blot, Immunoprecipitation. Results. Three hybridoma clones (A1, A2 and A3) producing mAbs specific to...
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| Date: | 2012 |
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| Main Authors: | , , , , , , , |
| Format: | Article |
| Language: | English |
| Published: |
Інститут молекулярної біології і генетики НАН України
2012
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| Series: | Вiopolymers and Cell |
| Subjects: | |
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| Journal Title: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Cite this: | Generation of monoclonal antibodies specific to ribosomal protein S6 kinase 1 / L.O. Savinska, O.M. Klipa, N.O. Demchuk, G.V. Ovcharenko, O.M. Malanchuk, I.O. Tykhonkova, S.S. Palchevskyy, V.V. Filonenko // Вiopolymers and Cell. — 2012. — Т. 28, № 6. — С. 424-428. — Бібліогр.: 22 назв. — англ. |
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Digital Library of Periodicals of National Academy of Sciences of Ukraine| Summary: | The aim of this study was to produce monoclonal antibodies directed to the N-terminal regulatory region of S6K1,
which shows very low homology to S6K2. Methods. Hybridoma technology, ELISA, Western blot, Immunoprecipitation. Results. Three hybridoma clones (A1, A2 and A3) producing mAbs specific to ribosomal protein S6
kinase 1 have been generated. Specificity of mAbs has been confirmed by Western blot and immunoprecipitation
technique. Conclusions. The obtained antibodies are suitable for elucidating signal transduction pathways
involving S6K1.
Keywords: ribosomal protein S6 kinase, mAbs. |
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