Generation of monoclonal antibodies specific to ribosomal protein S6 kinase 1

Generation of monoclonal antibodies specific to ribosomal protein S6 kinase 1

The aim of this study was to produce monoclonal antibodies directed to the N-terminal regulatory region of S6K1, which shows very low homology to S6K2. Methods. Hybridoma technology, ELISA, Western blot, Immunoprecipitation. Results. Three hybridoma clones (A1, A2 and A3) producing mAbs specific to...

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Bibliographic Details
Date:2012
Main Authors: Savinska, L.O., Klipa, O.M., Demchuk, N.O., Ovcharenko, G.V., Malanchuk, O.M., Tykhonkova, I.O., Palchevskyy, S.S., Filonenko, V.V.
Format: Article
Language:English
Published: Інститут молекулярної біології і генетики НАН України 2012
Series:Вiopolymers and Cell
Subjects:
Structure and Function of Biopolymers
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Journal Title:Digital Library of Periodicals of National Academy of Sciences of Ukraine
Cite this:Generation of monoclonal antibodies specific to ribosomal protein S6 kinase 1 / L.O. Savinska, O.M. Klipa, N.O. Demchuk, G.V. Ovcharenko, O.M. Malanchuk, I.O. Tykhonkova, S.S. Palchevskyy, V.V. Filonenko // Вiopolymers and Cell. — 2012. — Т. 28, № 6. — С. 424-428. — Бібліогр.: 22 назв. — англ.

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Digital Library of Periodicals of National Academy of Sciences of Ukraine

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