Natural variation of wheat cuticular waxes in relation to drought tolerance

Aims. The aim of our research is to investigate the role of wheat leaf cuticle in drought and heat protection by characterizing natural variability of cuticle components and revealing genetic and biochemical background of this variability in Australian wheat varieties. Methods. Cultivars with diff...

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Datum:2015
Hauptverfasser: Bi, H., Kovalchuk, N.V., Dias, D., Roessner, U., Langridge, P., Lopato, S., Borisjuk, N.V.
Format: Artikel
Sprache:English
Veröffentlicht: Інститут молекулярної біології і генетики НАН України 2015
Schriftenreihe:Фактори експериментальної еволюції організмів
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Назва журналу:Digital Library of Periodicals of National Academy of Sciences of Ukraine
Zitieren:Natural variation of wheat cuticular waxes in relation to drought tolerance / H. Bi, N.V. Kovalchuk, D. Dias, U. Roessner, P. Langridge, S. Lopato, N.V. Borisjuk // Фактори експериментальної еволюції організмів: Зб. наук. пр. — 2015. — Т. 16. — С. 174-178. — Бібліогр.: 9 назв. — англ.

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Digital Library of Periodicals of National Academy of Sciences of Ukraine
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Zusammenfassung:Aims. The aim of our research is to investigate the role of wheat leaf cuticle in drought and heat protection by characterizing natural variability of cuticle components and revealing genetic and biochemical background of this variability in Australian wheat varieties. Methods. Cultivars with different levels of drought tolerance were analyzed for cuticle wax composition by gas chromatography (GC–MS) and for cuticle structure properties by scanning electron microscopy (SEM). Results. Metabolomics analyses demonstrated signifi cant quantitative differences between drought-sensitive and drought-tolerant cultivars in several types of wax components. These data are complemented by differences in cuticle structure revealed by SEM. Ten wheat genes potentially involved in regulation of cuticle biosynthesis have been cloned and analysed for expression profi les in wheat by RT-qPCT. Conclusions. Acquired data are currently being used for identifi cation of enzymes and genes responsible for drought-related cuticular components. Keywords: wheat, drought tolerance, cuticle waxes, gene expression.