Цитогенетичні особливості калюсних культур тритикале озимого за дії осмотичного стресу
Aims. To study the effects of sublethal concentrations of mannitol on cytogenetic structure of cell populations of callus cultures of winter triticale (ЧTriticosecale Wittmack) during selection of resistant to osmotic stress forms. Methods. By cytological analysis method there were determined ploi...
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| Date: | 2015 |
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| Main Authors: | , , |
| Format: | Article |
| Language: | Ukrainian |
| Published: |
Інститут молекулярної біології і генетики НАН України
2015
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| Series: | Фактори експериментальної еволюції організмів |
| Subjects: | |
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| Journal Title: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Cite this: | Цитогенетичні особливості калюсних культур тритикале озимого за дії осмотичного стресу / С.В. Пикало, А.В. Бавол, О.В. Дубровна // Фактори експериментальної еволюції організмів: Зб. наук. пр. — 2015. — Т. 17. — С. 230-235. — Бібліогр.: 14 назв. — укр. |
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Digital Library of Periodicals of National Academy of Sciences of Ukraine| Summary: | Aims. To study the effects of sublethal concentrations of mannitol on cytogenetic structure of cell populations of callus
cultures of winter triticale (ЧTriticosecale Wittmack) during selection of resistant to osmotic stress forms. Methods.
By cytological analysis method there were determined ploidy level, frequency of structural chromosome aberrations
and mitotic abnormalities in the cells of callus cultures of winter triticale under sublethal concentrations of mannitol.
Results. Analysis of genetic structure of cell populations during cultivation showed a significant increase in aneuploidy
frequency and genome segregation that is shown growth of cell populations with reduced relative to the modal number
of chromosomes. Conclusions. It was established that sublethal concentrations of mannitol caused significant clastogenic
effect and turbagene disturbances in callus cells of triticale.
Keywords: ЧTriticosecale Wittmack, callus cultures, mannitol, cytological analysis, chromosome aberrations. |
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