Введення в культуру in vitro різних сортів сої (Glycine max (L.) Merr.), стерилізація та динаміка калюсоутворення
Aims. The aim of this study was the selection of the optimal culture conditions for introduction in vitro of different ukrainian soybean varieties (“Horol”, “Tanais”, “Terek”, “Kuban”, “Cheremosh granit”) and the investigation of callus formation on different types of explants. Methods. Нypocotyls...
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| Date: | 2015 |
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| Main Authors: | , , |
| Format: | Article |
| Language: | Ukrainian |
| Published: |
Інститут молекулярної біології і генетики НАН України
2015
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| Series: | Фактори експериментальної еволюції організмів |
| Subjects: | |
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| Journal Title: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Cite this: | Введення в культуру in vitro різних сортів сої (Glycine max (L.) Merr.), стерилізація та динаміка калюсоутворення / О.Ф. Забейда, В.П. Жук, В.Д. Науменко // Фактори експериментальної еволюції організмів: Зб. наук. пр. — 2015. — Т. 17. — С. 156-159. — Бібліогр.: 16 назв. — укр. |
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Digital Library of Periodicals of National Academy of Sciences of Ukraine| Summary: | Aims. The aim of this study was the selection of the optimal culture conditions for introduction in vitro of different ukrainian
soybean varieties (“Horol”, “Tanais”, “Terek”, “Kuban”, “Cheremosh granit”) and the investigation of callus formation on
different types of explants. Methods. Нypocotyls, internodes and embryos as explants were used. The highest frequency of
callus formation was observed for soybean explants such as hypocotyls and embryos (varieties “Horol”, “Tanais”, “Kuban”)
on the medium S1, S2, S3. Callus growth did not occur on the medium S4, S5, S6. Results. The highest frequency for
induction of callus was observed for soybean varieties «Horol», «Tanais», «Kuban» on the culture medium S1, S2, S3.
Conclusions. It was found that the effectiveness of callus formation depended of the mineral and phytohormonal medium
composition, type of explants and explants growth place Hypocotyls and embryos as explants were more effective for callus
induction than internodes. Medium S1 was the best medium for callus induction.
Keywords: Glycine max (L.) Merr., in vitro culture, callus induction. |
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