Експресія гена протонно-натрієвого обміннику HvNHX2 ячменю в рослинах тютюну покращує показники солестійкості
Aims. In this study, the gene encoding HvNHX2, a vacuolar Na+/H+ exchanger from barley was transferred to tobacco plants by Agrobacterium-mediated transformation in order to evaluate the improvement of salt tolerance. Methods. Gateaway cloning, RT-PCR and PCR, in vitro plant culture and transforma...
Saved in:
| Date: | 2015 |
|---|---|
| Main Authors: | , , |
| Format: | Article |
| Language: | Ukrainian |
| Published: |
Інститут молекулярної біології і генетики НАН України
2015
|
| Series: | Фактори експериментальної еволюції організмів |
| Subjects: | |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Journal Title: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Cite this: | Експресія гена протонно-натрієвого обміннику HvNHX2 ячменю в рослинах тютюну покращує показники солестійкості / С.В. Ісаєнков, О.Є. Краснопьорова, Я.Б. Блюм // Фактори експериментальної еволюції організмів: Зб. наук. пр. — 2015. — Т. 17. — С. 174-178. — Бібліогр.: 15 назв. — укр. |
Institution
Digital Library of Periodicals of National Academy of Sciences of Ukraine| Summary: | Aims. In this study, the gene encoding HvNHX2, a vacuolar Na+/H+ exchanger from barley was transferred to tobacco plants
by Agrobacterium-mediated transformation in order to evaluate the improvement of salt tolerance. Methods. Gateaway
cloning, RT-PCR and PCR, in vitro plant culture and transformation, physiological tests were combined to evaluate efficiency
of transformation and ability to tolerate high salt concentrations. Results. The significant improvement salt tolerance were
detected in transgenic tobacco lines after expression of HvNHX2 gene in these plants. The HvNHX2 expressing plants are
able to survive on lethal for the wild type concentrations of NaCl (250 mM). Conclusions. Suggested approach to improve
the salt tolerance of plants by gene expression one of key Na+/H+ antiporter has a great potential for the further application
in plant biotechnology and molecular breeding.
Keywords: salt tolerance, tobacco, Na+/H+ exchanger, plant transformation, HvNHX2, plant biotechnology. |
|---|