Низька геномна мінливість в культурі in vitro лінії кукурудзи Black Mexican Sweet Corn C456
An analysis of callus tissues of maize Black Mexican Sweet Corn C456 line was performed through RAPD-PCR. 275 amplicons were scored, and only 2 of them were polymorphic. The polymorphic fragments revealed a variability among the plants as well as between the plants and their calluses, that may all...
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| Date: | 2008 |
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| Main Authors: | , , , |
| Format: | Article |
| Language: | Ukrainian |
| Published: |
Видавничий дім "Академперіодика" НАН України
2008
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| Journal Title: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Cite this: | Низька геномна мінливість в культурі in vitro лінії кукурудзи Black Mexican Sweet Corn C456 / Д.М. Майданюк, І.О. Андрєєв, К.В. Спірідонова, В.А. Кунах // Доповіді Національної академії наук України. — 2008. — № 1. — С. 161-164. — Бібліогр.: 10 назв. — укp. |
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Digital Library of Periodicals of National Academy of Sciences of Ukraine| Summary: | An analysis of callus tissues of maize Black Mexican Sweet Corn C456 line was performed
through RAPD-PCR. 275 amplicons were scored, and only 2 of them were polymorphic. The
polymorphic fragments revealed a variability among the plants as well as between the plants and their calluses, that may allow one to assume the existence of “hot spots” with higher variability in maize genome. Blot-hybridization of one polymorphic RAPD-amplicon demonstrated the presence of its sequence in genomes of all objects under study, so the revealed changes are most
likely related to considerable differences in the amounts of PCR products. The obtained results suggest the low level of genome variability in maize tissue culture. |
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