Криоконсервирование культуры фибробластов эмбрионов человека

Cryoprotective properties of 1,2-propanediol (1,2-PD), oxyethylated glycerol (OEG; n=5) and fetal calf serum (FCS) when freeze-thaw human fetal fibroblast (HFF) culture were studied. It has been established that 1,2-PD and OEG have weak cryoprotective properties and do not provide the integrity of f...

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Bibliographische Detailangaben
Datum:2007
Hauptverfasser: Timon, V. V., Petrenko, T. F., Goncharuk, E. I.
Format: Artikel
Sprache:English
Veröffentlicht: Publishing House ‘Akademperiodyka’ of the National Academy of Sciences of Ukraine; Institute for Problems of Cryobiology and Cryomedicine 2007
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Online Zugang:https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/535
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Назва журналу:Problems of Cryobiology and Cryomedicine

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Problems of Cryobiology and Cryomedicine
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Zusammenfassung:Cryoprotective properties of 1,2-propanediol (1,2-PD), oxyethylated glycerol (OEG; n=5) and fetal calf serum (FCS) when freeze-thaw human fetal fibroblast (HFF) culture were studied. It has been established that 1,2-PD and OEG have weak cryoprotective properties and do not provide the integrity of functional activity of HEF cells. Cell cryopreservation in FCS provided maximum integrity of cells and their functional activity.