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There was studied the estradiol (E), progesterone (P) and testosterone (T) content in follicular fluid (FF) of human oocyte preovulatory follicles, native and low-temperature-stored one (-196°C). We have compared the effect of two freezing regimens (one-step immersion into liquid nitrogen and a two...
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Date: | 2004 |
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Main Authors: | , |
Format: | Article |
Language: | English |
Published: |
Publishing House ‘Akademperiodyka’ of the National Academy of Sciences of Ukraine; Institute for Problems of Cryobiology and Cryomedicine
2004
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Subjects: | |
Online Access: | https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/709 |
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Journal Title: | Problems of Cryobiology and Cryomedicine |
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Problems of Cryobiology and CryomedicineSummary: | There was studied the estradiol (E), progesterone (P) and testosterone (T) content in follicular fluid (FF) of human oocyte preovulatory follicles, native and low-temperature-stored one (-196°C). We have compared the effect of two freezing regimens (one-step immersion into liquid nitrogen and a two-step one with the preliminary exposure in nitrogen vapors) on the stability of concentration maintenance of these hormones in FF for 1 and 6 months. Stability of estradiol, progesterone and testosterone was shown to be kept within the whole observation period and to be independent upon the FL freezing regimen selected. |
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