Дослідження морфофункціональних характеристик кріоконсервованих культур клітин стромального походження

Mesenchymal stromal cells (MSCs) of bone marrow and chorion cell culture (CCC) were cryopreserved under protection of 10% DMSO and 20% fetal bovine serum using low cooling rate (1 deg/min) down to –80°C with following plunging into liquid nitrogen. The findings have shown than after low temperatu...

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Збережено в:
Бібліографічні деталі
Дата:2012
Автор: Volkova, N O.
Формат: Стаття
Мова:English
Опубліковано: Publishing House ‘Akademperiodyka’ of the National Academy of Sciences of Ukraine; Institute for Problems of Cryobiology and Cryomedicine 2012
Теми:
Онлайн доступ:https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/72
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Назва журналу:Problems of Cryobiology and Cryomedicine

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Problems of Cryobiology and Cryomedicine
Опис
Резюме:Mesenchymal stromal cells (MSCs) of bone marrow and chorion cell culture (CCC) were cryopreserved under protection of 10% DMSO and 20% fetal bovine serum using low cooling rate (1 deg/min) down to –80°C with following plunging into liquid nitrogen. The findings have shown than after low temperature storage both studied cultures kept the ability to proliferation and directed differentiation. Chorion cell culture was characterized with higher proliferative activity if compared with bone marrow MSCs. The ability to the directed differentiation towards adipo- and chondrogeneic lineages was higher for bone marrow MSCs versus CCC.