ВИЯВЛЕННЯ АНТИТІЛ ПРОТИ БЕНЗО[А]ПІРЕНУ В СИРОВАТЦІ КРОВІ ХВОРИХ НА РАК ЛЕГЕНІ ЗА ДОПОМОГОЮ ІМУНОФЕРМЕНТНОГО МЕТОДУ З ВИКОРИСТАННЯМ РЕКОМБІНАНТНИХ ІДІОТИПОВИХ ТА АНТИІДІОТИПОВИХ АНТИТІЛ
Summary. Aim of this study was to develop and apply an ELISA method for assessment of the levels of endogenous antibodies against benzo[a]pyrene (Bp) in blood sera of lung cancer (LC) patients. Materials and Methods: ELISA was developed and applied for the detection of endogenous idiotypic and anti-...
Збережено в:
| Дата: | 2023 |
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| Автори: | , , |
| Формат: | Стаття |
| Мова: | English |
| Опубліковано: |
PH Akademperiodyka
2023
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| Теми: | |
| Онлайн доступ: | https://exp-oncology.com.ua/index.php/Exp/article/view/2021-4-14 |
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| Назва журналу: | Experimental Oncology |
Репозитарії
Experimental Oncology| Резюме: | Summary. Aim of this study was to develop and apply an ELISA method for assessment of the levels of endogenous antibodies against benzo[a]pyrene (Bp) in blood sera of lung cancer (LC) patients. Materials and Methods: ELISA was developed and applied for the detection of endogenous idiotypic and anti-idiotypic antibodies against Bp in human blood sera using recombinant human idiotypic and anti-idiotypic antibodies against Bp. Results: Serum samples of LC patients (n = 22) and healthy donors (n = 24) were analyzed by the new method. Statistical analysis showed that in sera of LC patients the levels of endogenous idiotypic and anti-idiotypic antibodies against Bp were significantly higher than in healthy donors. A logistic regression model for the LC detection utilizing such predictors as the serum levels of idiotypic and anti-idiotypic antibodies against Bp, smoking status, and age, identified LC patients with 83% specificity and 82% sensitivity. Conclusion: The proposed method could be further developed as additional lung cancer screening tool. |
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