Effect of valproic acid and zebularine on SOCS-1 and SOCS-3 gene expression in colon carcinoma SW48 cell line
Summary. Background: Two epigenetic modifications such as histone acetylation and DNA methylation have been known as critical players of gene regulation. Hypermethylation and deacetylation of suppressors of cytokine signaling family SOCS-1 and SOCS-3 have been shown in many solid c...
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| Дата: | 2023 |
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PH Akademperiodyka
2023
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| Назва журналу: | Experimental Oncology |
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oai:ojs2.ex.aqua-time.com.ua:article-1762023-10-11T16:43:26Z Effect of valproic acid and zebularine on SOCS-1 and SOCS-3 gene expression in colon carcinoma SW48 cell line Effect of valproic acid and zebularine on SOCS-1 and SOCS-3 gene expression in colon carcinoma SW48 cell line Sanaei, M. Kavoosi, F. Behjoo, H. colon carcinoma, SOCS-1, SOCS-3, valproic acid, zebularine colon carcinoma, SOCS-1, SOCS-3, valproic acid, zebularine Summary. Background: Two epigenetic modifications such as histone acetylation and DNA methylation have been known as critical players of gene regulation. Hypermethylation and deacetylation of suppressors of cytokine signaling family SOCS-1 and SOCS-3 have been shown in many solid cancers. Previously, we evaluated the effect of 5-aza-2′-deoxycytidine and valproic acid on hepatocellular carcinoma and colon cancer cells. Aim: The present study was designed to assess the effect of valproic acid in comparison to zebularine on SOCS-1 and SOCS-3 gene expression, cell growth inhibition and apoptosis induction in colon carcinoma SW48 cell line. Materials and Methods: SW48 cells were treated with valproic acid or zebularine for 24 h and 48 h. The effect of the compounds on cell viability, SOCS-1 and SOCS-3 gene expression, and apoptosis induction was evaluated. Reverse transcription polymerase chain reaction analysis and flow cytometry were applied. Results: Both agents inhibited cell growth in a time- and dose-dependent fashion. The apoptotic effect was observed in cells treated with valproic acid (7.5 μM) but not zebularine (75 μM). The valproic acid but not zebularine upregulated SOCS-1 and SOCS-3 gene expression. Conclusion: Epigenetic modulation can reactivate silenced tumor suppressor genes SOCS-1 and SOCS-3 through histone acetylation resulting in apoptosis induction. Summary. Background: Two epigenetic modifications such as histone acetylation and DNA methylation have been known as critical players of gene regulation. Hypermethylation and deacetylation of suppressors of cytokine signaling family SOCS-1 and SOCS-3 have been shown in many solid cancers. Previously, we evaluated the effect of 5-aza-2′-deoxycytidine and valproic acid on hepatocellular carcinoma and colon cancer cells. Aim: The present study was designed to assess the effect of valproic acid in comparison to zebularine on SOCS-1 and SOCS-3 gene expression, cell growth inhibition and apoptosis induction in colon carcinoma SW48 cell line. Materials and Methods: SW48 cells were treated with valproic acid or zebularine for 24 h and 48 h. The effect of the compounds on cell viability, SOCS-1 and SOCS-3 gene expression, and apoptosis induction was evaluated. Reverse transcription polymerase chain reaction analysis and flow cytometry were applied. Results: Both agents inhibited cell growth in a time- and dose-dependent fashion. The apoptotic effect was observed in cells treated with valproic acid (7.5 μM) but not zebularine (75 μM). The valproic acid but not zebularine upregulated SOCS-1 and SOCS-3 gene expression. Conclusion: Epigenetic modulation can reactivate silenced tumor suppressor genes SOCS-1 and SOCS-3 through histone acetylation resulting in apoptosis induction. PH Akademperiodyka 2023-05-31 Article Article application/pdf https://exp-oncology.com.ua/index.php/Exp/article/view/2020-3-13 10.32471/exp-oncology.2312-8852.vol-42-no-3.15113 Experimental Oncology; Vol. 42 No. 3 (2020): Experimental Oncology; 183-187 Експериментальна онкологія; Том 42 № 3 (2020): Експериментальна онкологія; 183-187 2312-8852 1812-9269 10.32471/exp-oncology.2312-8852.vol-42-no-3 en https://exp-oncology.com.ua/index.php/Exp/article/view/2020-3-13/2020-3-13 Copyright (c) 2023 Experimental Oncology https://creativecommons.org/licenses/by-nc/4.0/ |
| institution |
Experimental Oncology |
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|
| datestamp_date |
2023-10-11T16:43:26Z |
| collection |
OJS |
| language |
English |
| topic |
colon carcinoma SOCS-1 SOCS-3 valproic acid zebularine |
| spellingShingle |
colon carcinoma SOCS-1 SOCS-3 valproic acid zebularine Sanaei, M. Kavoosi, F. Behjoo, H. Effect of valproic acid and zebularine on SOCS-1 and SOCS-3 gene expression in colon carcinoma SW48 cell line |
| topic_facet |
colon carcinoma SOCS-1 SOCS-3 valproic acid zebularine colon carcinoma SOCS-1 SOCS-3 valproic acid zebularine |
| format |
Article |
| author |
Sanaei, M. Kavoosi, F. Behjoo, H. |
| author_facet |
Sanaei, M. Kavoosi, F. Behjoo, H. |
| author_sort |
Sanaei, M. |
| title |
Effect of valproic acid and zebularine on SOCS-1 and SOCS-3 gene expression in colon carcinoma SW48 cell line |
| title_short |
Effect of valproic acid and zebularine on SOCS-1 and SOCS-3 gene expression in colon carcinoma SW48 cell line |
| title_full |
Effect of valproic acid and zebularine on SOCS-1 and SOCS-3 gene expression in colon carcinoma SW48 cell line |
| title_fullStr |
Effect of valproic acid and zebularine on SOCS-1 and SOCS-3 gene expression in colon carcinoma SW48 cell line |
| title_full_unstemmed |
Effect of valproic acid and zebularine on SOCS-1 and SOCS-3 gene expression in colon carcinoma SW48 cell line |
| title_sort |
effect of valproic acid and zebularine on socs-1 and socs-3 gene expression in colon carcinoma sw48 cell line |
| title_alt |
Effect of valproic acid and zebularine on SOCS-1 and SOCS-3 gene expression in colon carcinoma SW48 cell line |
| description |
Summary. Background: Two epigenetic modifications such as histone acetylation and DNA methylation have been known as critical players of gene regulation. Hypermethylation and deacetylation of suppressors of cytokine signaling family SOCS-1 and SOCS-3 have been shown in many solid cancers. Previously, we evaluated the effect of 5-aza-2′-deoxycytidine and valproic acid on hepatocellular carcinoma and colon cancer cells. Aim: The present study was designed to assess the effect of valproic acid in comparison to zebularine on SOCS-1 and SOCS-3 gene expression, cell growth inhibition and apoptosis induction in colon carcinoma SW48 cell line. Materials and Methods: SW48 cells were treated with valproic acid or zebularine for 24 h and 48 h. The effect of the compounds on cell viability, SOCS-1 and SOCS-3 gene expression, and apoptosis induction was evaluated. Reverse transcription polymerase chain reaction analysis and flow cytometry were applied. Results: Both agents inhibited cell growth in a time- and dose-dependent fashion. The apoptotic effect was observed in cells treated with valproic acid (7.5 μM) but not zebularine (75 μM). The valproic acid but not zebularine upregulated SOCS-1 and SOCS-3 gene expression. Conclusion: Epigenetic modulation can reactivate silenced tumor suppressor genes SOCS-1 and SOCS-3 through histone acetylation resulting in apoptosis induction. |
| publisher |
PH Akademperiodyka |
| publishDate |
2023 |
| url |
https://exp-oncology.com.ua/index.php/Exp/article/view/2020-3-13 |
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2025-07-17T12:16:09Z |
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2025-07-17T12:16:09Z |
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