Analysis of immunoglobulin heavy variable chain rearrangement in chronic lymphocytic leukemia patients among Chornobyl clean-up workers
Summary. Background: A number of epidemiological studies have shown an elevated radiation-associated risk for chronic lymphocytic leukemia (CLL). The aim of the paper was to analyze immunoglobulin heavy variable chain (IGHV) rearrangement and IGHV usage in CLL cases associated with ionizing...
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| Дата: | 2023 |
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PH Akademperiodyka
2023
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| Назва журналу: | Experimental Oncology |
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Experimental Oncology| id |
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Experimental Oncology |
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2023-10-11T16:43:26Z |
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English |
| topic |
chronic lymphocytic leukemia immunoglobulin variable heavy chain (IGHV) rearrangement ionizing radiation |
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chronic lymphocytic leukemia immunoglobulin variable heavy chain (IGHV) rearrangement ionizing radiation Abramenko, I.V. Bilous, N.I. Chumak, A.A. Dyagil, I.S. Martina, Z.V. Analysis of immunoglobulin heavy variable chain rearrangement in chronic lymphocytic leukemia patients among Chornobyl clean-up workers |
| topic_facet |
chronic lymphocytic leukemia immunoglobulin variable heavy chain (IGHV) rearrangement ionizing radiation chronic lymphocytic leukemia immunoglobulin variable heavy chain (IGHV) rearrangement ionizing radiation |
| format |
Article |
| author |
Abramenko, I.V. Bilous, N.I. Chumak, A.A. Dyagil, I.S. Martina, Z.V. |
| author_facet |
Abramenko, I.V. Bilous, N.I. Chumak, A.A. Dyagil, I.S. Martina, Z.V. |
| author_sort |
Abramenko, I.V. |
| title |
Analysis of immunoglobulin heavy variable chain rearrangement in chronic lymphocytic leukemia patients among Chornobyl clean-up workers |
| title_short |
Analysis of immunoglobulin heavy variable chain rearrangement in chronic lymphocytic leukemia patients among Chornobyl clean-up workers |
| title_full |
Analysis of immunoglobulin heavy variable chain rearrangement in chronic lymphocytic leukemia patients among Chornobyl clean-up workers |
| title_fullStr |
Analysis of immunoglobulin heavy variable chain rearrangement in chronic lymphocytic leukemia patients among Chornobyl clean-up workers |
| title_full_unstemmed |
Analysis of immunoglobulin heavy variable chain rearrangement in chronic lymphocytic leukemia patients among Chornobyl clean-up workers |
| title_sort |
analysis of immunoglobulin heavy variable chain rearrangement in chronic lymphocytic leukemia patients among chornobyl clean-up workers |
| title_alt |
Analysis of immunoglobulin heavy variable chain rearrangement in chronic lymphocytic leukemia patients among Chornobyl clean-up workers |
| description |
Summary. Background: A number of epidemiological studies have shown an elevated radiation-associated risk for chronic lymphocytic leukemia (CLL). The aim of the paper was to analyze immunoglobulin heavy variable chain (IGHV) rearrangement and IGHV usage in CLL cases associated with ionizing radiation (IR) exposure. Materials and Methods: Samples of 76 clean-up workers of Chornobyl Nuclear Power Plant accident of 1986 (the main group) and 194 non-exposed patients (the control group) were analyzed. Two groups of CLL patients were comparable by gender (all patients were male), age, and place of residence (rural or urban). Results: Some features of IR-associated CLL cases as compared to CLL cases in patients without history of IR exposure were revealed. Among unmutated IGHV sequences, IGHV1 genes were less commonly used (29.4% vs 48.6%; p = 0.018), while the frequency of IGHD6 genes was higher (23.5% vs 10%; p = 0.029). The unmutated IGHV sequences did not use IGHD3-16 gene (0% vs 7.9%, p = 0.038). Mutated IGHV sequences were less frequently expressed IGHV3 genes (44% vs 68.5%; p = 0.037) due low representation of IGHV3-21 (4% vs 11.1%) and IGHV3-23 (0% vs 11.1%) genes; did not use IGHD3-22 gene (0% vs 18.5%, p = 0.025); and have signs of positive selection in the HCDR regions (Σ = 0.5029 ± 0.155 vs –0.0539 ± 0.14; p = 0.013). Conclusions: The revealed differences in IGHV gene usage and B-cell receptor structure in the main and the control groups of CLL patients indirectly indicate a change in the spectrum of antigens associated with CLL under IR exposure. The possible antigenic drivers associated with CLL associated with IR exposure are discussed. |
| publisher |
PH Akademperiodyka |
| publishDate |
2023 |
| url |
https://exp-oncology.com.ua/index.php/Exp/article/view/2020-3-16 |
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AT abramenkoiv analysisofimmunoglobulinheavyvariablechainrearrangementinchroniclymphocyticleukemiapatientsamongchornobylcleanupworkers AT bilousni analysisofimmunoglobulinheavyvariablechainrearrangementinchroniclymphocyticleukemiapatientsamongchornobylcleanupworkers AT chumakaa analysisofimmunoglobulinheavyvariablechainrearrangementinchroniclymphocyticleukemiapatientsamongchornobylcleanupworkers AT dyagilis analysisofimmunoglobulinheavyvariablechainrearrangementinchroniclymphocyticleukemiapatientsamongchornobylcleanupworkers AT martinazv analysisofimmunoglobulinheavyvariablechainrearrangementinchroniclymphocyticleukemiapatientsamongchornobylcleanupworkers |
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2025-07-17T12:16:11Z |
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oai:ojs2.ex.aqua-time.com.ua:article-1792023-10-11T16:43:26Z Analysis of immunoglobulin heavy variable chain rearrangement in chronic lymphocytic leukemia patients among Chornobyl clean-up workers Analysis of immunoglobulin heavy variable chain rearrangement in chronic lymphocytic leukemia patients among Chornobyl clean-up workers Abramenko, I.V. Bilous, N.I. Chumak, A.A. Dyagil, I.S. Martina, Z.V. chronic lymphocytic leukemia, immunoglobulin variable heavy chain (IGHV) rearrangement, ionizing radiation chronic lymphocytic leukemia, immunoglobulin variable heavy chain (IGHV) rearrangement, ionizing radiation Summary. Background: A number of epidemiological studies have shown an elevated radiation-associated risk for chronic lymphocytic leukemia (CLL). The aim of the paper was to analyze immunoglobulin heavy variable chain (IGHV) rearrangement and IGHV usage in CLL cases associated with ionizing radiation (IR) exposure. Materials and Methods: Samples of 76 clean-up workers of Chornobyl Nuclear Power Plant accident of 1986 (the main group) and 194 non-exposed patients (the control group) were analyzed. Two groups of CLL patients were comparable by gender (all patients were male), age, and place of residence (rural or urban). Results: Some features of IR-associated CLL cases as compared to CLL cases in patients without history of IR exposure were revealed. Among unmutated IGHV sequences, IGHV1 genes were less commonly used (29.4% vs 48.6%; p = 0.018), while the frequency of IGHD6 genes was higher (23.5% vs 10%; p = 0.029). The unmutated IGHV sequences did not use IGHD3-16 gene (0% vs 7.9%, p = 0.038). Mutated IGHV sequences were less frequently expressed IGHV3 genes (44% vs 68.5%; p = 0.037) due low representation of IGHV3-21 (4% vs 11.1%) and IGHV3-23 (0% vs 11.1%) genes; did not use IGHD3-22 gene (0% vs 18.5%, p = 0.025); and have signs of positive selection in the HCDR regions (Σ = 0.5029 ± 0.155 vs –0.0539 ± 0.14; p = 0.013). Conclusions: The revealed differences in IGHV gene usage and B-cell receptor structure in the main and the control groups of CLL patients indirectly indicate a change in the spectrum of antigens associated with CLL under IR exposure. The possible antigenic drivers associated with CLL associated with IR exposure are discussed. Summary. Background: A number of epidemiological studies have shown an elevated radiation-associated risk for chronic lymphocytic leukemia (CLL). The aim of the paper was to analyze immunoglobulin heavy variable chain (IGHV) rearrangement and IGHV usage in CLL cases associated with ionizing radiation (IR) exposure. Materials and Methods: Samples of 76 clean-up workers of Chornobyl Nuclear Power Plant accident of 1986 (the main group) and 194 non-exposed patients (the control group) were analyzed. Two groups of CLL patients were comparable by gender (all patients were male), age, and place of residence (rural or urban). Results: Some features of IR-associated CLL cases as compared to CLL cases in patients without history of IR exposure were revealed. Among unmutated IGHV sequences, IGHV1 genes were less commonly used (29.4% vs 48.6%; p = 0.018), while the frequency of IGHD6 genes was higher (23.5% vs 10%; p = 0.029). The unmutated IGHV sequences did not use IGHD3-16 gene (0% vs 7.9%, p = 0.038). Mutated IGHV sequences were less frequently expressed IGHV3 genes (44% vs 68.5%; p = 0.037) due low representation of IGHV3-21 (4% vs 11.1%) and IGHV3-23 (0% vs 11.1%) genes; did not use IGHD3-22 gene (0% vs 18.5%, p = 0.025); and have signs of positive selection in the HCDR regions (Σ = 0.5029 ± 0.155 vs –0.0539 ± 0.14; p = 0.013). Conclusions: The revealed differences in IGHV gene usage and B-cell receptor structure in the main and the control groups of CLL patients indirectly indicate a change in the spectrum of antigens associated with CLL under IR exposure. The possible antigenic drivers associated with CLL associated with IR exposure are discussed. PH Akademperiodyka 2023-05-31 Article Article application/pdf https://exp-oncology.com.ua/index.php/Exp/article/view/2020-3-16 10.32471/exp-oncology.2312-8852.vol-42-no-3.14839 Experimental Oncology; Vol. 42 No. 3 (2020): Experimental Oncology; 172-177 Експериментальна онкологія; Том 42 № 3 (2020): Експериментальна онкологія; 172-177 2312-8852 1812-9269 10.32471/exp-oncology.2312-8852.vol-42-no-3 en https://exp-oncology.com.ua/index.php/Exp/article/view/2020-3-16/2020-3-16 Copyright (c) 2023 Experimental Oncology https://creativecommons.org/licenses/by-nc/4.0/ |