Tumor-associated redox state in metastatic colorectal cancer
Summary. The high incidence of recurrence and metastasizing in colorectal cancer (CRC) poses the challenge for the improvement in long-term treatment outcome. Aim: To determine the major indicators of redox-formative molecules in the tissue of metastatic CRC (mCRC), stages Т2–4N0–2M0G2–3, n...
Збережено в:
| Дата: | 2023 |
|---|---|
| Автори: | , , , , |
| Формат: | Стаття |
| Мова: | English |
| Опубліковано: |
PH Akademperiodyka
2023
|
| Теми: | |
| Онлайн доступ: | https://exp-oncology.com.ua/index.php/Exp/article/view/2019-2-9 |
| Теги: |
Додати тег
Немає тегів, Будьте першим, хто поставить тег для цього запису!
|
| Назва журналу: | Experimental Oncology |
Репозитарії
Experimental Oncology| id |
oai:ojs2.ex.aqua-time.com.ua:article-250 |
|---|---|
| record_format |
ojs |
| institution |
Experimental Oncology |
| baseUrl_str |
|
| datestamp_date |
2023-10-11T16:41:24Z |
| collection |
OJS |
| language |
English |
| topic |
“free iron” EPR lactoferrin matrix metalloproteinase-2 matrix metalloproteinase-9 metastatic colorectal cancer NO spin traps superoxide radicals |
| spellingShingle |
“free iron” EPR lactoferrin matrix metalloproteinase-2 matrix metalloproteinase-9 metastatic colorectal cancer NO spin traps superoxide radicals Burlaka, A.P. Ganusevich, І.І. Burlaka, А.А. Virko, S.V. Kolesnik, О.О. Tumor-associated redox state in metastatic colorectal cancer |
| topic_facet |
“free iron” EPR lactoferrin matrix metalloproteinase-2 matrix metalloproteinase-9 metastatic colorectal cancer NO spin traps superoxide radicals “free iron” EPR lactoferrin matrix metalloproteinase-2 matrix metalloproteinase-9 metastatic colorectal cancer NO spin traps superoxide radicals |
| format |
Article |
| author |
Burlaka, A.P. Ganusevich, І.І. Burlaka, А.А. Virko, S.V. Kolesnik, О.О. |
| author_facet |
Burlaka, A.P. Ganusevich, І.І. Burlaka, А.А. Virko, S.V. Kolesnik, О.О. |
| author_sort |
Burlaka, A.P. |
| title |
Tumor-associated redox state in metastatic colorectal cancer |
| title_short |
Tumor-associated redox state in metastatic colorectal cancer |
| title_full |
Tumor-associated redox state in metastatic colorectal cancer |
| title_fullStr |
Tumor-associated redox state in metastatic colorectal cancer |
| title_full_unstemmed |
Tumor-associated redox state in metastatic colorectal cancer |
| title_sort |
tumor-associated redox state in metastatic colorectal cancer |
| title_alt |
Tumor-associated redox state in metastatic colorectal cancer |
| description |
Summary. The high incidence of recurrence and metastasizing in colorectal cancer (CRC) poses the challenge for the improvement in long-term treatment outcome. Aim: To determine the major indicators of redox-formative molecules in the tissue of metastatic CRC (mCRC), stages Т2–4N0–2M0G2–3, namely the rate of superoxide radical (SR) generation, nitric oxide (NO) content, the activity of matrix metalloproteinases (MMP), lactoferrin (LF) content, and “free” iron and their association with some clinical and pathological characteristics of the patients. Materials and Methods: mCRC samples from 51 patients were analyzed (stage II, 31 patients; stage III, 20 patients). The LF and “free” iron were assessed by electron paramagnetic resonance (EPR) at the temperature of 77 °K. The rate of SR and NO generation was determined with spin traps (ТЕМРО-Н, diethyl dithiocarbamate). The activity of MMP-2 and -9 was measured by gelatin zymography using SDS-polyacrylamide gel electrophoresis. Ki-67 expression was analyzed by immunofluorescence technique. Results: In tumors with metastases into the regional lymph nodes (N1–2 category), SR generation rate was 2.2-fold higher than in the tumors categorized as N0. In G3 mCRC, SR generation rate was 1.7-fold higher than in G2-tumors (р < 0.05). The rate of SR generation correlated inversely with differentiation grade of the tumor (r = –0.61; р < 0.05). MMP-2 and -9 activities in mCRC tissue correlated with SR generation rate and NO level (r = 0.44 ÷ 0.53, p < 0.05). The direct correlation between LF content and the stage of the disease (r = 0.42) and “free” iron content (r = 0.61) was demonstrated while the correlation between LF content and tumor differentiation grade was inverse (r = –0.57; р < 0.05). Conclusions: The altered tumor-associated redox state in mCRC tissue contributes to the increased cell proliferation and formation of aggressive phenotype of the tumor. The assays for the content of redox-formative components in mCRC may be used as additional prognostic markers of the course of the disease in CRC patients. |
| publisher |
PH Akademperiodyka |
| publishDate |
2023 |
| url |
https://exp-oncology.com.ua/index.php/Exp/article/view/2019-2-9 |
| work_keys_str_mv |
AT burlakaap tumorassociatedredoxstateinmetastaticcolorectalcancer AT ganusevichíí tumorassociatedredoxstateinmetastaticcolorectalcancer AT burlakaaa tumorassociatedredoxstateinmetastaticcolorectalcancer AT virkosv tumorassociatedredoxstateinmetastaticcolorectalcancer AT kolesnikoo tumorassociatedredoxstateinmetastaticcolorectalcancer |
| first_indexed |
2025-07-17T12:17:02Z |
| last_indexed |
2025-07-17T12:17:02Z |
| _version_ |
1837896432112107521 |
| spelling |
oai:ojs2.ex.aqua-time.com.ua:article-2502023-10-11T16:41:24Z Tumor-associated redox state in metastatic colorectal cancer Tumor-associated redox state in metastatic colorectal cancer Burlaka, A.P. Ganusevich, І.І. Burlaka, А.А. Virko, S.V. Kolesnik, О.О. “free iron”, EPR, lactoferrin, matrix metalloproteinase-2, matrix metalloproteinase-9, metastatic colorectal cancer, NO, spin traps, superoxide radicals “free iron”, EPR, lactoferrin, matrix metalloproteinase-2, matrix metalloproteinase-9, metastatic colorectal cancer, NO, spin traps, superoxide radicals Summary. The high incidence of recurrence and metastasizing in colorectal cancer (CRC) poses the challenge for the improvement in long-term treatment outcome. Aim: To determine the major indicators of redox-formative molecules in the tissue of metastatic CRC (mCRC), stages Т2–4N0–2M0G2–3, namely the rate of superoxide radical (SR) generation, nitric oxide (NO) content, the activity of matrix metalloproteinases (MMP), lactoferrin (LF) content, and “free” iron and their association with some clinical and pathological characteristics of the patients. Materials and Methods: mCRC samples from 51 patients were analyzed (stage II, 31 patients; stage III, 20 patients). The LF and “free” iron were assessed by electron paramagnetic resonance (EPR) at the temperature of 77 °K. The rate of SR and NO generation was determined with spin traps (ТЕМРО-Н, diethyl dithiocarbamate). The activity of MMP-2 and -9 was measured by gelatin zymography using SDS-polyacrylamide gel electrophoresis. Ki-67 expression was analyzed by immunofluorescence technique. Results: In tumors with metastases into the regional lymph nodes (N1–2 category), SR generation rate was 2.2-fold higher than in the tumors categorized as N0. In G3 mCRC, SR generation rate was 1.7-fold higher than in G2-tumors (р < 0.05). The rate of SR generation correlated inversely with differentiation grade of the tumor (r = –0.61; р < 0.05). MMP-2 and -9 activities in mCRC tissue correlated with SR generation rate and NO level (r = 0.44 ÷ 0.53, p < 0.05). The direct correlation between LF content and the stage of the disease (r = 0.42) and “free” iron content (r = 0.61) was demonstrated while the correlation between LF content and tumor differentiation grade was inverse (r = –0.57; р < 0.05). Conclusions: The altered tumor-associated redox state in mCRC tissue contributes to the increased cell proliferation and formation of aggressive phenotype of the tumor. The assays for the content of redox-formative components in mCRC may be used as additional prognostic markers of the course of the disease in CRC patients. Summary. The high incidence of recurrence and metastasizing in colorectal cancer (CRC) poses the challenge for the improvement in long-term treatment outcome. Aim: To determine the major indicators of redox-formative molecules in the tissue of metastatic CRC (mCRC), stages Т2–4N0–2M0G2–3, namely the rate of superoxide radical (SR) generation, nitric oxide (NO) content, the activity of matrix metalloproteinases (MMP), lactoferrin (LF) content, and “free” iron and their association with some clinical and pathological characteristics of the patients. Materials and Methods: mCRC samples from 51 patients were analyzed (stage II, 31 patients; stage III, 20 patients). The LF and “free” iron were assessed by electron paramagnetic resonance (EPR) at the temperature of 77 °K. The rate of SR and NO generation was determined with spin traps (ТЕМРО-Н, diethyl dithiocarbamate). The activity of MMP-2 and -9 was measured by gelatin zymography using SDS-polyacrylamide gel electrophoresis. Ki-67 expression was analyzed by immunofluorescence technique. Results: In tumors with metastases into the regional lymph nodes (N1–2 category), SR generation rate was 2.2-fold higher than in the tumors categorized as N0. In G3 mCRC, SR generation rate was 1.7-fold higher than in G2-tumors (р < 0.05). The rate of SR generation correlated inversely with differentiation grade of the tumor (r = –0.61; р < 0.05). MMP-2 and -9 activities in mCRC tissue correlated with SR generation rate and NO level (r = 0.44 ÷ 0.53, p < 0.05). The direct correlation between LF content and the stage of the disease (r = 0.42) and “free” iron content (r = 0.61) was demonstrated while the correlation between LF content and tumor differentiation grade was inverse (r = –0.57; р < 0.05). Conclusions: The altered tumor-associated redox state in mCRC tissue contributes to the increased cell proliferation and formation of aggressive phenotype of the tumor. The assays for the content of redox-formative components in mCRC may be used as additional prognostic markers of the course of the disease in CRC patients. PH Akademperiodyka 2023-06-05 Article Article application/pdf https://exp-oncology.com.ua/index.php/Exp/article/view/2019-2-9 10.32471/exp-oncology.2312-8852.vol-41-no-2.13128 Experimental Oncology; Vol. 41 No. 2 (2019): Experimental Oncology; 148-152 Експериментальна онкологія; Том 41 № 2 (2019): Експериментальна онкологія; 148-152 2312-8852 1812-9269 10.32471/exp-oncology.2312-8852.vol-41-no-2 en https://exp-oncology.com.ua/index.php/Exp/article/view/2019-2-9/2019-2-9 Copyright (c) 2023 Experimental Oncology https://creativecommons.org/licenses/by-nc/4.0/ |