Vitamin Е activates expression of С/EBP alpha transcription factor and G-CSF receptor in leukemic K562 cells
Summary. Background: Chronic myeloid leukemia (CML) is a clonal hematopoietic stem cell disorder associated with the activity of BCR-ABL fusion oncogene. Tyrosine kinase inhibitors are the current treatment of CML, but secondary mutations finally contribute to therapy resistance and blast crisis of...
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PH Akademperiodyka
2018
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Experimental Oncology| id |
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Experimental Oncology |
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2025-04-30T11:55:13Z |
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English |
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C/EBP alpha transcription factor chronic myeloid leukemia G-CSFR granulocytic differentiation vitamin E. |
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C/EBP alpha transcription factor chronic myeloid leukemia G-CSFR granulocytic differentiation vitamin E. Shvachko, L.P. Zavelevich, M.P. Gluzman, D.F. Kravchuk, I.V. Telegeev, G.D. Vitamin Е activates expression of С/EBP alpha transcription factor and G-CSF receptor in leukemic K562 cells |
| topic_facet |
C/EBP alpha transcription factor chronic myeloid leukemia G-CSFR granulocytic differentiation vitamin E. C/EBP alpha transcription factor chronic myeloid leukemia G-CSFR granulocytic differentiation vitamin E. |
| format |
Article |
| author |
Shvachko, L.P. Zavelevich, M.P. Gluzman, D.F. Kravchuk, I.V. Telegeev, G.D. |
| author_facet |
Shvachko, L.P. Zavelevich, M.P. Gluzman, D.F. Kravchuk, I.V. Telegeev, G.D. |
| author_sort |
Shvachko, L.P. |
| title |
Vitamin Е activates expression of С/EBP alpha transcription factor and G-CSF receptor in leukemic K562 cells |
| title_short |
Vitamin Е activates expression of С/EBP alpha transcription factor and G-CSF receptor in leukemic K562 cells |
| title_full |
Vitamin Е activates expression of С/EBP alpha transcription factor and G-CSF receptor in leukemic K562 cells |
| title_fullStr |
Vitamin Е activates expression of С/EBP alpha transcription factor and G-CSF receptor in leukemic K562 cells |
| title_full_unstemmed |
Vitamin Е activates expression of С/EBP alpha transcription factor and G-CSF receptor in leukemic K562 cells |
| title_sort |
vitamin е activates expression of с/ebp alpha transcription factor and g-csf receptor in leukemic k562 cells |
| title_alt |
Vitamin Е activates expression of С/EBP alpha transcription factor and G-CSF receptor in leukemic K562 cells |
| description |
Summary. Background: Chronic myeloid leukemia (CML) is a clonal hematopoietic stem cell disorder associated with the activity of BCR-ABL fusion oncogene. Tyrosine kinase inhibitors are the current treatment of CML, but secondary mutations finally contribute to therapy resistance and blast crisis of the disease. The search for the novel compounds for the effective control of CML is now in the spotlight. The progression of CML to blast crisis is correlated with down-modulation of C/EBP alpha. Therefore, C/EBP alpha may be considered as a putative target in differentiation therapies in myeloid leukemias. The aim of the study was to assess the potential of vitamin E as the possible inducer of C/EBP alpha expression in BCR-ABL-positive CML K562 cells. Materials and Methods: RNA extracted from K562 cells cultured with valproic acid or vitamin E was converted to cDNA, RT-PCR reactions were carried out using HotStarTaq DNA polymerase with primers for C/EBP alpha and granulocyte colony-stimulating factor receptor (G-CSFR). Results: We have not found detectable expression of C/EBP alpha in K562 cells. Upon 48-h culture with vitamin E at a dose of 100 µM, K562 cells expressed both C/EBP alpha and G-CSFR. Conclusion: Vitamin E restored the expression of C/EBP alpha mRNA in chronic myelogenous leukemia K562 cells. In this setting, G-CSFR expression in vitamin E treated K562 cells seems to suggest the activation to granulocytic differentiation. It should be further elucidated whether such effects of vitamin E on C/EBP alpha transcription factor are direct or mediated indirectly due to antioxidant properties of vitamin E. |
| publisher |
PH Akademperiodyka |
| publishDate |
2018 |
| url |
https://exp-oncology.com.ua/index.php/Exp/article/view/2018-4-13 |
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AT shvachkolp vitamineactivatesexpressionofsebpalphatranscriptionfactorandgcsfreceptorinleukemick562cells AT zavelevichmp vitamineactivatesexpressionofsebpalphatranscriptionfactorandgcsfreceptorinleukemick562cells AT gluzmandf vitamineactivatesexpressionofsebpalphatranscriptionfactorandgcsfreceptorinleukemick562cells AT kravchukiv vitamineactivatesexpressionofsebpalphatranscriptionfactorandgcsfreceptorinleukemick562cells AT telegeevgd vitamineactivatesexpressionofsebpalphatranscriptionfactorandgcsfreceptorinleukemick562cells |
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2025-07-17T12:17:28Z |
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2025-07-17T12:17:28Z |
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oai:ojs2.ex.aqua-time.com.ua:article-2912025-04-30T11:55:13Z Vitamin Е activates expression of С/EBP alpha transcription factor and G-CSF receptor in leukemic K562 cells Vitamin Е activates expression of С/EBP alpha transcription factor and G-CSF receptor in leukemic K562 cells Shvachko, L.P. Zavelevich, M.P. Gluzman, D.F. Kravchuk, I.V. Telegeev, G.D. C/EBP alpha transcription factor, chronic myeloid leukemia, G-CSFR, granulocytic differentiation, vitamin E. C/EBP alpha transcription factor, chronic myeloid leukemia, G-CSFR, granulocytic differentiation, vitamin E. Summary. Background: Chronic myeloid leukemia (CML) is a clonal hematopoietic stem cell disorder associated with the activity of BCR-ABL fusion oncogene. Tyrosine kinase inhibitors are the current treatment of CML, but secondary mutations finally contribute to therapy resistance and blast crisis of the disease. The search for the novel compounds for the effective control of CML is now in the spotlight. The progression of CML to blast crisis is correlated with down-modulation of C/EBP alpha. Therefore, C/EBP alpha may be considered as a putative target in differentiation therapies in myeloid leukemias. The aim of the study was to assess the potential of vitamin E as the possible inducer of C/EBP alpha expression in BCR-ABL-positive CML K562 cells. Materials and Methods: RNA extracted from K562 cells cultured with valproic acid or vitamin E was converted to cDNA, RT-PCR reactions were carried out using HotStarTaq DNA polymerase with primers for C/EBP alpha and granulocyte colony-stimulating factor receptor (G-CSFR). Results: We have not found detectable expression of C/EBP alpha in K562 cells. Upon 48-h culture with vitamin E at a dose of 100 µM, K562 cells expressed both C/EBP alpha and G-CSFR. Conclusion: Vitamin E restored the expression of C/EBP alpha mRNA in chronic myelogenous leukemia K562 cells. In this setting, G-CSFR expression in vitamin E treated K562 cells seems to suggest the activation to granulocytic differentiation. It should be further elucidated whether such effects of vitamin E on C/EBP alpha transcription factor are direct or mediated indirectly due to antioxidant properties of vitamin E. Summary. Background: Chronic myeloid leukemia (CML) is a clonal hematopoietic stem cell disorder associated with the activity of BCR-ABL fusion oncogene. Tyrosine kinase inhibitors are the current treatment of CML, but secondary mutations finally contribute to therapy resistance and blast crisis of the disease. The search for the novel compounds for the effective control of CML is now in the spotlight. The progression of CML to blast crisis is correlated with down-modulation of C/EBP alpha. Therefore, C/EBP alpha may be considered as a putative target in differentiation therapies in myeloid leukemias. The aim of the study was to assess the potential of vitamin E as the possible inducer of C/EBP alpha expression in BCR-ABL-positive CML K562 cells. Materials and Methods: RNA extracted from K562 cells cultured with valproic acid or vitamin E was converted to cDNA, RT-PCR reactions were carried out using HotStarTaq DNA polymerase with primers for C/EBP alpha and granulocyte colony-stimulating factor receptor (G-CSFR). Results: We have not found detectable expression of C/EBP alpha in K562 cells. Upon 48-h culture with vitamin E at a dose of 100 µM, K562 cells expressed both C/EBP alpha and G-CSFR. Conclusion: Vitamin E restored the expression of C/EBP alpha mRNA in chronic myelogenous leukemia K562 cells. In this setting, G-CSFR expression in vitamin E treated K562 cells seems to suggest the activation to granulocytic differentiation. It should be further elucidated whether such effects of vitamin E on C/EBP alpha transcription factor are direct or mediated indirectly due to antioxidant properties of vitamin E. PH Akademperiodyka 2018-12-22 Article Article application/pdf https://exp-oncology.com.ua/index.php/Exp/article/view/2018-4-13 Experimental Oncology; Vol. 40 No. 4 (2018): Experimental Oncology; 328-331 Експериментальна онкологія; Том 40 № 4 (2018): Експериментальна онкологія; 328-331 2312-8852 1812-9269 en https://exp-oncology.com.ua/index.php/Exp/article/view/2018-4-13/2018-4-13 Copyright (c) 2023 Experimental Oncology https://creativecommons.org/licenses/by-nc/4.0/ |