Вiк-залежнi змiни Раман- та ІЧ-спектрiв кровi людини
Blood analysis by spectroscopic techniques can provide important information about biochemistry and life processes in it. Blood indices are highly variable, and plenty of factors influence them. The present work describes the combination of two methods – IR and Raman spectroscopies of blood applied...
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| Date: | 2019 |
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Publishing house "Academperiodika"
2019
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| Online Access: | https://ujp.bitp.kiev.ua/index.php/ujp/article/view/2019021 |
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ujp2-article-20190212019-04-20T07:46:31Z Age-Related Changes in FTIR and Raman Spectra of Human Blood Вiк-залежнi змiни Раман- та ІЧ-спектрiв кровi людини Makhnii, T. Ilchenko, O. Reynt, A. Pilgun, Y. Kutsyk, A. Krasnenkov, D. Ivasyuk, M. Kukharskyy, V. human blood age Raman spectroscopy ATR-FTIR spectroscopy Partial Least Squares (PLS) analysis - Blood analysis by spectroscopic techniques can provide important information about biochemistry and life processes in it. Blood indices are highly variable, and plenty of factors influence them. The present work describes the combination of two methods – IR and Raman spectroscopies of blood applied to investigate gerontology issues. We carried out a pilot study of 74 blood samples. The donors were differentiated by age with the Partial Least Squares (PLS) analysis of Raman and IR spectra. Analyzing the principal component spectra obtained during PLS processn the most illustrative bands were found in the intervals 2860–3030 cm−1, 1370–1620 cm−1, 1020–1220 cm−1 and in 1650–1530 cm−1, 1380–1360 cm−1, 1220–1200 cm−1, 1002–1004 cm−1, 760–750 cm−1 in IR and Raman spectral regions, respectively. Calibration models obtained via the PLS analysis of blood vibrational spectra provide the accuracy of age determination around ±15 years from FTIR data and around ±20 years from Raman data. Though such calibrations cannot be used for the precise determination of age, the age-related changes of blood do really exist and can be detected from vibrational spectra. Розглянуто можливостi застосування Раман- та IЧ-спектроскопiї для аналiзу вiкових змiн кровi людини. Дослiджено 74 зразки кровi пацiєнтiв рiзних вiкових груп. До дослiджуваних спектрiв було застосовано PLS-аналiз. Виявлено недостатню точнiсть методу для визначення вiку. При цьому були знайденi вiк-залежнi дiлянки спектрiв. Було охарактеризовано основнi дiлянки спектрiв, що показали змiни, залежнi вiд вiку, вiдповiдно до PLS-аналiзу та лiтературних даних. В статтi наведено приклади характерних Раман- та IЧ-спектрiв. Значення всiх знайдених пiкiв Раман- та IЧ-спектрiв зiбранi та описанi у таблицi вiдповiдно до лiтературних даних. Знайдено, що основнi вiдмiнностi стосуються пiкiв, якi несуть iнформацiю про гемоглобiн та його похiднi. Було зроблено висновок щодо необхiдностi проведення дослiду з бiльшою кiлькiстю зразкiв. Publishing house "Academperiodika" 2019-01-04 Article Article application/pdf https://ujp.bitp.kiev.ua/index.php/ujp/article/view/2019021 10.15407/ujpe61.10.0853 Ukrainian Journal of Physics; Vol. 61 No. 10 (2016); 853 Український фізичний журнал; Том 61 № 10 (2016); 853 2071-0194 2071-0186 10.15407/ujpe61.10 en https://ujp.bitp.kiev.ua/index.php/ujp/article/view/2019021/900 Copyright (c) 2019 Bogolyubov Institute for Theoretical Physics, National Academy of Sciences of Ukraine |
| institution |
Ukrainian Journal of Physics |
| collection |
OJS |
| language |
English |
| topic |
human blood age Raman spectroscopy ATR-FTIR spectroscopy Partial Least Squares (PLS) analysis - |
| spellingShingle |
human blood age Raman spectroscopy ATR-FTIR spectroscopy Partial Least Squares (PLS) analysis - Makhnii, T. Ilchenko, O. Reynt, A. Pilgun, Y. Kutsyk, A. Krasnenkov, D. Ivasyuk, M. Kukharskyy, V. Вiк-залежнi змiни Раман- та ІЧ-спектрiв кровi людини |
| topic_facet |
human blood age Raman spectroscopy ATR-FTIR spectroscopy Partial Least Squares (PLS) analysis - |
| format |
Article |
| author |
Makhnii, T. Ilchenko, O. Reynt, A. Pilgun, Y. Kutsyk, A. Krasnenkov, D. Ivasyuk, M. Kukharskyy, V. |
| author_facet |
Makhnii, T. Ilchenko, O. Reynt, A. Pilgun, Y. Kutsyk, A. Krasnenkov, D. Ivasyuk, M. Kukharskyy, V. |
| author_sort |
Makhnii, T. |
| title |
Вiк-залежнi змiни Раман- та ІЧ-спектрiв кровi людини |
| title_short |
Вiк-залежнi змiни Раман- та ІЧ-спектрiв кровi людини |
| title_full |
Вiк-залежнi змiни Раман- та ІЧ-спектрiв кровi людини |
| title_fullStr |
Вiк-залежнi змiни Раман- та ІЧ-спектрiв кровi людини |
| title_full_unstemmed |
Вiк-залежнi змiни Раман- та ІЧ-спектрiв кровi людини |
| title_sort |
вiк-залежнi змiни раман- та іч-спектрiв кровi людини |
| title_alt |
Age-Related Changes in FTIR and Raman Spectra of Human Blood |
| description |
Blood analysis by spectroscopic techniques can provide important information about biochemistry and life processes in it. Blood indices are highly variable, and plenty of factors influence them. The present work describes the combination of two methods – IR and Raman spectroscopies of blood applied to investigate gerontology issues. We carried out a pilot study of 74 blood samples. The donors were differentiated by age with the Partial Least Squares (PLS) analysis of Raman and IR spectra. Analyzing the principal component spectra obtained during PLS processn the most illustrative bands were found in the intervals 2860–3030 cm−1, 1370–1620 cm−1, 1020–1220 cm−1 and in 1650–1530 cm−1, 1380–1360 cm−1, 1220–1200 cm−1, 1002–1004 cm−1, 760–750 cm−1 in IR and Raman spectral regions, respectively. Calibration models obtained via the PLS analysis of blood vibrational spectra provide the accuracy of age determination around ±15 years from FTIR data and around ±20 years from Raman data. Though such calibrations cannot be used for the precise determination of age, the age-related changes of blood do really exist and can be detected from vibrational spectra. |
| publisher |
Publishing house "Academperiodika" |
| publishDate |
2019 |
| url |
https://ujp.bitp.kiev.ua/index.php/ujp/article/view/2019021 |
| work_keys_str_mv |
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| first_indexed |
2023-03-24T08:56:51Z |
| last_indexed |
2023-03-24T08:56:51Z |
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