The effect of hypoxia on photocytotoxicity of TICS tricarbocyanine dye in vitro
Aim: To evaluate the effect of cell oxygenation on photocytotoxicity of a novel tricarbocyanine indolenine dye covalently bound to glucose (TICS). Methods: HeLa cells were incubated with 5 µM TICS, 2 h later irradiated by laser at 740 nm with a light dose of 10 J/cm2, delivered at a power density of...
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| Date: | 2006 |
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| Main Authors: | , , , , , , , |
| Format: | Article |
| Language: | English |
| Published: |
Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
2006
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| Series: | Experimental Oncology |
| Subjects: | |
| Online Access: | http://dspace.nbuv.gov.ua/handle/123456789/137550 |
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| Journal Title: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Cite this: | The effect of hypoxia on photocytotoxicity of TICS tricarbocyanine dye in vitro / Y.P. Istomin, E.N. Alexandrova, E.A. Zhavrid, E.S. Voropay, M.P. Samtsov, K.N. Kaplevsky, A.P. Lugovsky, A.А. Lugovsky // Experimental Oncology. — 2006. — Т. 28, № 1. — С. 80-82. — Бібліогр.: 16 назв. — англ. |
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Digital Library of Periodicals of National Academy of Sciences of Ukraine| Summary: | Aim: To evaluate the effect of cell oxygenation on photocytotoxicity of a novel tricarbocyanine indolenine dye covalently bound to glucose (TICS). Methods: HeLa cells were incubated with 5 µM TICS, 2 h later irradiated by laser at 740 nm with a light dose of 10 J/cm2, delivered at a power density of 10, 20, 25 or 30 mW/cm2, in air or in argon atmosphere, and then scored for viability. Results: The photocytotoxicity of TICS increased dramatically as the power density was reduced. Under hypoxia TICS-photosensitized cell death was determined but its value was lowered, compared to photoirradiation in the air. Conclusion: Photosensitizing effect of TICS is only partially dependent on the oxygenation of tumor cells. |
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