Practical approach to quantification of mRNA abundance using RT-qPCR, normalization of experimental data and MIQE
Reverse transcription and quantitative polymerase chain reaction (RT-qPCR) has become the most common method for characterizing expression patterns of individual mRNAs due to a large dynamic range of linear quantification, high speed, sensitivity, resolution and cost-effectiveness. However, the comp...
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| Datum: | 2016 |
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| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | English |
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Інститут молекулярної біології і генетики НАН України
2016
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| Schriftenreihe: | Вiopolymers and Cell |
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| Online Zugang: | http://dspace.nbuv.gov.ua/handle/123456789/152828 |
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| Назва журналу: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Zitieren: | Practical approach to quantification of mRNA abundance using RT-qPCR, normalization of experimental data and MIQE / M.Yu. Obolenskaya, A.V. Kuklin, R.R. Rodrigez, O.P. Martsenyuk, K.L. Korneyeva, V.A. Docenko, O.O. Draguschenko // Вiopolymers and Cell. — 2016. — Т. 32, № 3. — С. 161-172. — Бібліогр.: 46 назв. — англ. |