Expression and purification of full-length Alanyl-tRNA-synthetase from Thermus thermophilus HB27
Aim. To gain insight into structural and functional properties of alanyl-tRNA,synthetase (AlaRS), we genetically engineered constructs for expression and purification of full-length AlaRS and checked its activity in aminoacylation assays. Methods. The genomic DNA for the AlaS gene from the T. thermo...
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| Datum: | 2018 |
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| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | English |
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Інститут молекулярної біології і генетики НАН України
2018
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| Schriftenreihe: | Вiopolymers and Cell |
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| Online Zugang: | http://dspace.nbuv.gov.ua/handle/123456789/154370 |
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| Назва журналу: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Zitieren: | Expression and purification of full-length Alanyl-tRNA-synthetase from Thermus thermophilus HB27 / M.Yu. Rybak, A.E. Priss, O.I. Gudzera, A.O. Kovalchuk, I.A. Kryklyvyi, M.A. Tukalo // Вiopolymers and Cell. — 2018. — Т. 34, № 6. — С. 435-444. — Бібліогр.: 29 назв. — англ. |