Ідентифікація ARS-послідовності флавіногенних дріжджів Pichia guilliermondii
An autonomously replicating sequence adjacent to the RfBl gene encoding GTP cyclohydrolase II of the yeast Pichia guilliermondii was identified by transformation experiments. Detailed sequence analysis unveiled two potential ARS elements located 5' and 3' of the RIBI open reading frame. Th...
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| Date: | 2000 |
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| Main Authors: | , |
| Format: | Article |
| Language: | Ukrainian |
| Published: |
Інститут молекулярної біології і генетики НАН України
2000
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| Series: | Биополимеры и клетка |
| Subjects: | |
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| Journal Title: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Cite this: | Ідентифікація ARS-послідовності флавіногенних дріжджів Pichia guilliermondii / А.Я. Вороновський // Биополимеры и клетка. — 2000. — Т. 16, № 1. — С. 46-52. — Бібліогр.: 26 назв. — укр. |
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Digital Library of Periodicals of National Academy of Sciences of Ukraine| Summary: | An autonomously replicating sequence adjacent to the RfBl gene encoding GTP cyclohydrolase II of the yeast Pichia guilliermondii was identified by transformation experiments. Detailed sequence analysis unveiled two potential ARS elements located 5' and 3' of the RIBI open reading frame. The chromosomal fragment containing the ARS-like sequence 3' to the RIBI structural gene, called PgARS, conferred autonomous replication to hybrid plasmids and high transformation frequencies (104 –105 transformants/ug DNA) in Pichia guilliermondii. Based on the PgARS element a series of E. coli–P. guilliermondii shuttle vectors was developed. |
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