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Preliminary incubation of human and bovine erythrocytes in 0.4 and 1.0 M NaCl, correspondingly, results in the formation of a resistant cell state to the following 4.0 M NaCl effect. Maximum erythrocyte shrinking at the stage of preliminary incubation in moderately hypertonic media was shown as corr...
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| Date: | 2007 |
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| Main Authors: | , , |
| Format: | Article |
| Language: | English |
| Published: |
Publishing House ‘Akademperiodyka’ of the National Academy of Sciences of Ukraine; Institute for Problems of Cryobiology and Cryomedicine
2007
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| Subjects: | |
| Online Access: | https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/479 |
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| Journal Title: | Problems of Cryobiology and Cryomedicine |
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Problems of Cryobiology and Cryomedicine| Summary: | Preliminary incubation of human and bovine erythrocytes in 0.4 and 1.0 M NaCl, correspondingly, results in the formation of a resistant cell state to the following 4.0 M NaCl effect. Maximum erythrocyte shrinking at the stage of preliminary incubation in moderately hypertonic media was shown as correlating with the minimum hemolysis level under cell transfer into 4.0 M NaCl-containing medium. At 37°C the level of minimum hypertonic hemolysis and minimum hematocrit value for bovine erythrocytes is lower that the similar ones, obtained for human erythrocytes. The reduction of experimental temperature down to 0°C results in hemolysis level and human erythrocyte volume decrease compared to 37°C. |
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