Apoptosis-inducing effect of spiroaminopyrimidine analogue in NB4 leukemia cells via down-regulation of BIRC5 expression
Summary. Aim: It has been reported that spiroaminopyrimidine derivatives inhibited the growth and proliferation of various cancer cell lines. In the present study, we evaluated cytotoxic and apoptosis-inducing effects of 2,4-diamino-1,3-diazaspiro[5.5]-9-tert-butyl-2, 4-diene-5-carbonitril (9-tBAP)...
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| Дата: | 2023 |
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PH Akademperiodyka
2023
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oai:ojs2.ex.aqua-time.com.ua:article-2152023-10-11T16:42:06Z Apoptosis-inducing effect of spiroaminopyrimidine analogue in NB4 leukemia cells via down-regulation of BIRC5 expression Apoptosis-inducing effect of spiroaminopyrimidine analogue in NB4 leukemia cells via down-regulation of BIRC5 expression Dehghan Nayeri, M.J. Mahdavi, M. Fazeli, H. Hosseinpour Feizi, M.A. apoptosis, cell cycle, NB4 cell line, spiroaminopyrimidine apoptosis, cell cycle, NB4 cell line, spiroaminopyrimidine Summary. Aim: It has been reported that spiroaminopyrimidine derivatives inhibited the growth and proliferation of various cancer cell lines. In the present study, we evaluated cytotoxic and apoptosis-inducing effects of 2,4-diamino-1,3-diazaspiro[5.5]-9-tert-butyl-2, 4-diene-5-carbonitril (9-tBAP) on NB4 acute promyelocytic leukemia (APL) cells. Materials and Methods: The cells were treated with 10–100 µM of 9-tBAP. Cytotoxic activity of the compound was measured using the MTT assay. Apoptosis was investigated by Hoechst 33258 staining as well as by Annexin V/PI double staining. Results: The compound under study was found to be highly active cell growth inhibitor with IC50 of 30.0 ± 3.5 µM inducing apoptosis in NB4 cells. Cell cycle analysis by flow cytometry showed a time-dependent increase in sub-G1 cell population. Real-time polymerase chain reaction analysis revealed that the treatment with the compound down-regulated the BIRC5 expression in a time-dependent manner. Conclusion: 9-tBAP displayed potent anti-leukemic activity in vitro thus warranting further investigation. Summary. Aim: It has been reported that spiroaminopyrimidine derivatives inhibited the growth and proliferation of various cancer cell lines. In the present study, we evaluated cytotoxic and apoptosis-inducing effects of 2,4-diamino-1,3-diazaspiro[5.5]-9-tert-butyl-2, 4-diene-5-carbonitril (9-tBAP) on NB4 acute promyelocytic leukemia (APL) cells. Materials and Methods: The cells were treated with 10–100 µM of 9-tBAP. Cytotoxic activity of the compound was measured using the MTT assay. Apoptosis was investigated by Hoechst 33258 staining as well as by Annexin V/PI double staining. Results: The compound under study was found to be highly active cell growth inhibitor with IC50 of 30.0 ± 3.5 µM inducing apoptosis in NB4 cells. Cell cycle analysis by flow cytometry showed a time-dependent increase in sub-G1 cell population. Real-time polymerase chain reaction analysis revealed that the treatment with the compound down-regulated the BIRC5 expression in a time-dependent manner. Conclusion: 9-tBAP displayed potent anti-leukemic activity in vitro thus warranting further investigation. PH Akademperiodyka 2023-06-02 Article Article application/pdf https://exp-oncology.com.ua/index.php/Exp/article/view/2019-4-5 10.32471/exp-oncology.2312-8852.vol-41-no-4.13749 Experimental Oncology; Vol. 41 No. 4 (2019): Experimental Oncology; 312-317 Експериментальна онкологія; Том 41 № 4 (2019): Експериментальна онкологія; 312-317 2312-8852 1812-9269 10.32471/exp-oncology.2312-8852.vol-41-no-4 en https://exp-oncology.com.ua/index.php/Exp/article/view/2019-4-5/2019-4-5 Copyright (c) 2023 Experimental Oncology https://creativecommons.org/licenses/by-nc/4.0/ |
| institution |
Experimental Oncology |
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| datestamp_date |
2023-10-11T16:42:06Z |
| collection |
OJS |
| language |
English |
| topic |
apoptosis cell cycle NB4 cell line spiroaminopyrimidine |
| spellingShingle |
apoptosis cell cycle NB4 cell line spiroaminopyrimidine Dehghan Nayeri, M.J. Mahdavi, M. Fazeli, H. Hosseinpour Feizi, M.A. Apoptosis-inducing effect of spiroaminopyrimidine analogue in NB4 leukemia cells via down-regulation of BIRC5 expression |
| topic_facet |
apoptosis cell cycle NB4 cell line spiroaminopyrimidine apoptosis cell cycle NB4 cell line spiroaminopyrimidine |
| format |
Article |
| author |
Dehghan Nayeri, M.J. Mahdavi, M. Fazeli, H. Hosseinpour Feizi, M.A. |
| author_facet |
Dehghan Nayeri, M.J. Mahdavi, M. Fazeli, H. Hosseinpour Feizi, M.A. |
| author_sort |
Dehghan Nayeri, M.J. |
| title |
Apoptosis-inducing effect of spiroaminopyrimidine analogue in NB4 leukemia cells via down-regulation of BIRC5 expression |
| title_short |
Apoptosis-inducing effect of spiroaminopyrimidine analogue in NB4 leukemia cells via down-regulation of BIRC5 expression |
| title_full |
Apoptosis-inducing effect of spiroaminopyrimidine analogue in NB4 leukemia cells via down-regulation of BIRC5 expression |
| title_fullStr |
Apoptosis-inducing effect of spiroaminopyrimidine analogue in NB4 leukemia cells via down-regulation of BIRC5 expression |
| title_full_unstemmed |
Apoptosis-inducing effect of spiroaminopyrimidine analogue in NB4 leukemia cells via down-regulation of BIRC5 expression |
| title_sort |
apoptosis-inducing effect of spiroaminopyrimidine analogue in nb4 leukemia cells via down-regulation of birc5 expression |
| title_alt |
Apoptosis-inducing effect of spiroaminopyrimidine analogue in NB4 leukemia cells via down-regulation of BIRC5 expression |
| description |
Summary. Aim: It has been reported that spiroaminopyrimidine derivatives inhibited the growth and proliferation of various cancer cell lines. In the present study, we evaluated cytotoxic and apoptosis-inducing effects of 2,4-diamino-1,3-diazaspiro[5.5]-9-tert-butyl-2, 4-diene-5-carbonitril (9-tBAP) on NB4 acute promyelocytic leukemia (APL) cells. Materials and Methods: The cells were treated with 10–100 µM of 9-tBAP. Cytotoxic activity of the compound was measured using the MTT assay. Apoptosis was investigated by Hoechst 33258 staining as well as by Annexin V/PI double staining. Results: The compound under study was found to be highly active cell growth inhibitor with IC50 of 30.0 ± 3.5 µM inducing apoptosis in NB4 cells. Cell cycle analysis by flow cytometry showed a time-dependent increase in sub-G1 cell population. Real-time polymerase chain reaction analysis revealed that the treatment with the compound down-regulated the BIRC5 expression in a time-dependent manner. Conclusion: 9-tBAP displayed potent anti-leukemic activity in vitro thus warranting further investigation. |
| publisher |
PH Akademperiodyka |
| publishDate |
2023 |
| url |
https://exp-oncology.com.ua/index.php/Exp/article/view/2019-4-5 |
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